ENSA (AF067170) Human Untagged Clone

CAT#: SC102099

(untagged)-Human alpha endosulfine mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENSA
• Synonyms: ARPP-19e
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102099 sequence for AF067170 edited (data generated by NextGen Sequencing)
  ATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAGGAGAAGCAGGAC
  ACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAA
  TACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAA
  GGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAG
  CAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCA
  CAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGGTAA
  
  Clone variation with respect to AF067170.1
  279 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF067170 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCAGCGCAGGTTGTCCC
  CGTTTCCCCTCCCCCTTCCCTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTC
  CCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGCTGGAGACCGGCGAGG
  AGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGC
  TAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGA
  GACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGA
  TGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACA
  TCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGG
  GGTAACCTGAGCCCCCCTCTCCTCCCCTTCCTCAACCACTGGACGTTTATATATTATAGG
  CAGGGATGAAATGGGCACCTAGTCAGATCTTCTCAGCTTGCTAGCCAGAAATGACTGTGA
  TTCTGCTGGGGGCTGCTGAGAAGGTAATGTANGTTGAAAAGGGGCTCTAAGTTTATTTAT
  TTCGTTAGATTGACACTTCCACACACTCCCTGTAGTCCAGGTAGGGCCCANAAATAGGAA
  AGGCTANGATTGGGATATGCTGCANATGCTTTTTTTGTGTGAGAAACTGGAGAGATGTGA
  TTTCTCCTTTTGGGAGAGATGTCCCANNATTGATTANGCTGAGCCTTGGGAATAGTTTGG
  CAGGTTAACATNCCAGGCTACCTAACGTANNTGGAAAGTANNATGATGAGACAATGTTCT
  TGCTCTTAGAGGTCTGTCCTTAGCTGTATTGCTCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF067170 unedited
  NGGTACGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTATTTTTTTTTTTTTTTTTGAAAAAAACGTACAAAATTATATATATATTTATATAT
  ATAATAACATGACATATCTATGTACAACATGGCTGGGACAGTTGAAGAAACTATACAATG
  GTGTTCAGCATTTTCCCCTTCCCAGATGGACTTTAAGGATGACAGCATGAGGAAATGGAG
  CAAGAAACACAAAAATTATATACAATTACAAGTGACAGTCAAGGAGTTTGGGGACCAGGG
  AGTCCAGGGATCCTGCTCTCTCCATTCCTTCCTCACCAACTTTCTCCTATCCAATTTGAA
  TGACAGCCTGAACACTGAATGGCCAGTCAGGAGAAAGGCATATACACACCTCATCCCCCC
  ACATGCACATCAGCAAGTCTATCAGTCATTCTCATTGGGCCAAATGTTTGGCATATCAAA
  ATTTGTGATGTGAGAGGGCAAGAGGATTACAGAATCTTGCTCTCTTGAGCATTAGTTCTC
  ACCCTGGTCCTCTCTGTCATAACTAGCAAGTTGCGTTCAATAAAATTCTAGCCAAGCTGG
  TGGTAACTAGATTGAAGATGCTGGTCACCTGCAACCCCTTTCCTCCACCTGTTCATGAGT
  CAGCATGGCTACCTCTCCTCATGATAAAAGAGCTGGGTTGCTGAATTCTGGGGATGCAGA
  GATTTTTTAGTCTTTCTTCTCTTCCTCCCTAACCACTTTCCCCAGCTTCCTCCAGGTGAA
  TAATCTCCTAAGACCTGTTTCTCCACCCTGCAGAATACTTACCCCCAGGGGCTAGCTCAG
  TTAACCCCTCTCCTCTTTCCTAACAATATACCTGGCATCAGACTGCATTTGGAACAAGGG
  TCCAAACATGTGCCTGACTG
  
• Restriction Sites: NotI-NotI
• ACCN: AF067170
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF067170.1, AAD32454.1
• RefSeq Size: 2520 bp
• RefSeq ORF: 2520 bp
• Locus ID: 2029
• Cytogenetics: 1q21.3
• Domains: endosulfine
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'