POLR2J2 (NM_032958) Human Untagged Clone

CAT#: SC102102

POLR2J2 (untagged)-Human DNA directed RNA polymerase II polypeptide J-related gene (POLR2J2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2J2
• Synonyms: DNA directed RNA polymerase II polypeptide J-related; HRPB11B; MGC54043; MGC105050; polymerase (RNA) II (DNA directed) polypeptide J2; RPB11, HRPB11B, MGC54043, MGC105050; RPB11b1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032958, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGATCACCATTAACAAGGACA
  CCAAGGTACCCAAGGCCTGCTTATTCACCATCAACAAAGAAGACCACACACTGGGAAACATCATTAAATC
  ACGTGCCTGCTTCCCCTTCGCCTTCTGCCGTGATTGTCAGTTTCCTGAGGCCTCCCCAGCCACGCTTCCT
  GTACAGCCTGCAGAACTCTGCCCCAGAGCACATCAGCTATGTGCCCCAGCTCTCAAACGACACCTTGGCG
  GGGAGGCTCACCCTGTCCACCTTCACGCTGGAGCAGCCTCTAGGCCAGTTCAGCAGCCACAACATCTCTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032958 unedited
  CGTCGCCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGGACGCA
  ACGGCGGCGGGAGCATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCG
  AGAAGATCACCATTAACAAGGACACCAAGGTACCCAATGCCTGCTTATTCACCATCAACA
  AAGAAGACCACACACTGGGAAACATCATTAAATTACGTGCCTGCTTCCCCTTCGCCTTCT
  GCCGTGATTGTCAGTTTCCTGAGGCCTCCCCAGCCACGCTTCCTGTACAGCCTGCAGAAC
  TCTGCCCCAGAGCACATCAGCTATGTGCCCCAGCTCTCAAACGACACCTTGGCGGGGAGG
  CTCACCCTGTCCACCTTCACGCTGGAGCAGCCTCTAGGCCAGTTCAGCAGCCACAACATC
  TCTGACTTGGATACCATCTGGCTGGTGGTGGCCCTCAGCAACGCCACCCAGAGCTTCACG
  GCCCCACGGACAAACCAGGACATCCCTGCTCCTGCCAACTTCTCCCAGAGGGGCTACTAT
  CTCACACTGAGGGCCAACCGGGTGCTGTACCAGACCAGAGGCCAGCTCCATGTCCTCCGC
  GTCGGCAATGATACCCACTGCCAACCAACAAAAATTGGCTGCAACCATCCCCTACCAGGA
  CCCGGCCCCTACAGGGTGAAGTTCCTGGTGATGAATGACGAAGGACCCGTGGCTGAAACA
  AATGGTCCAGCGACACTCGCCTGCAGCAAGCCCAGCACTTTGGGCTGTCCCCGGCCCCCA
  NAGCCCGGCACCGTGGTCATAATGGCCCTCCTGTTTATNCTCCTGGCCGGCCTCCTCACG
  GGACTCCTTGCCTGGGCTCATATACCCCTGCTTCAAAAGCTTGAGGAGCCCTTCCCTATC
  AGGCCCCCAGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032958
• Insert Size: 3830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032958.3, NP_116580.2
• RefSeq Size: 1552 bp
• RefSeq ORF: 351 bp
• Locus ID: 246721
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2), also called beta, differs in the 3' coding region and UTR, compared to variant 1. Isoform 2 has a distinct C-terminus and is shorter than isoform 1.