Dystrobrevin alpha (DTNA) (NM_032978) Human Untagged Clone

CAT#: SC102213

DTNA (untagged)-Human dystrobrevin, alpha (DTNA), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DTNA
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032978, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTGAAGATAGTGGGAAAAGAGGAAATACCATGGCAGAAAGAAGACAGCTGTTTGCAGAGATGAGGG
  CTCAAGATCTGGATCGCATCCGACTCTCCACCTACAGAACAGCATGCAAGCTTAGGTTTGTTCAGAAGAA
  ATGCAATTTGCACCTGGTGGACATATGGAATGTCATAGAAGCATTGCGGGAAAATGCTCTGAACAACCTG
  GACCCAAACACTGAACTCAACGTGTCCCGCTTAGAGGCTGTGCTCTCCACTATTTTTTACCAGCTCAACA
  AACGGATGCCAACCACTCACCAAATCCATGTGGAGCAGTCCATCAGCCTCCTCCTTAACTTCCTGCTTGC
  AGCGTTTGATCCGGAAGGCCATGGTAAAATTTCAGTATTTGCTGTCAAAATGGCTTTAGCCACATTGTGT
  GGAGGGAAGATCATGGACAAATTAAGATATATTTTCTCAATGATTTCTGACTCCAGTGGGGTGATGGTTT
  ATGGACGATATGACCAATTCCTTCGGGAAGTTCTCAAACTACCCACGGCAGTTTTTGAAGGTCCTTCATT
  TGGTTACACAGAACAGTCAGCCAGATCCTGTTTCTCCCAACAGAAAAAAGTCACGTTAAATGGTTTCTTG
  GACACGCTTATGTCAGATCCTCCCCCGCAGTGTCTGGTCTGGTTGCCTCTTCTGCATCGACTAGCAAATG
  TGGAAAATGTCTTCCATCCGGTTGAGTGTTCCTACTGCCACAGTGAGAGTATGATGGGATTTCGCTACCG
  ATGCCAACAGTGTCACAATTACCAGCTCTGTCAGGACTGCTTCTGGAGGGGACATGCCGGTGGTTCTCAT
  AGCAACCAGCACCAAATGAAAGAGTACACGTCATGGAAATCACCTGCTAAGAAGCTGACTAATGCATTAA
  GCAAGTCCCTGAGCTGTGCTTCCAGCCGTGAACCTTTGCACCCCATGTTCCCAGATCAGCCTGAGAAGCC
  ACTCAACTTGGCTCACATCGTGCCTCCCAGACCTGTAACCAGCATGAACGACACCCTGTTCTCCCACTCT
  GTTCCCTCCTCAGGAAGTCCTTTTATTACCAGGAGCTCTCCTCCCAAGGACAGTGAAGTAGAGCAGAACA
  AACTGCTGGCTAGGGCTGCTCCAGCTTTTCTGAAGGGCAAAGGGATACAGTACAGCCTGAATGTGGCAGA
  CAGGCTAGCTGATGAACATGTTCTCATCGGGTTGTATGTCAACATGCTCCGGAACAACCCCTCATGCATG
  CTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAGCAG
  AGTCCTCTTCGTCTCAGCCACCTCAGCAGAGAAGTGCTCCTGACATCTCTTTCACCATCGATGCGAATAA
  GCAGCAAAGGCAGCTGATTGCTGAGCTAGAAAACAAGAACAGAGAAATCTTACAGGAGATCCAGAGACTT
  CGGCTAGAGCATGAACAAGCTTCTCAGCCCACGCCAGAGAAGGCACAGCAAAACCCCACCCTGCTGGCAG
  AACTCCGGCTCCTCAGACAGCGCAAAGATGAGCTGGAACAGAGAATGTCTGCTCTCCAGGAGAGCCGGAG
  AGAGCTAATGGTCCAGTTGGAGGGTCTCATGAAGCTACTAAAGGAAGAAGAACTGAAGCAGGGAGTAAGT
  TATGTCCCCTACTGCAGGTCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032978 unedited
  GGCACTAATTGTAAACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGCTGGCAGCAGCCAGCCTCTGCTGTGCCCACATGACCCACAACTCTG
  GCAGCGGACCCGGCACTTCCAACATTATTAAATAATAAGAAAGCGGCTCCTACTCCAGGC
  TCAAACCTCCCTGCAGACCAATGGACACCTTCTAAGAGTTTGGCGAGTCAGTGACTGAAG
  CGCCCGTCCATTCCAAGATAAATAGGATTTACCAATCCTTGGATGAAGTGCTTGGGAAGT
  CTTTAAGTGCCATAATCAACTGCCATTTCAAAGAATATAGATGGTTTTGAAAAGTTCATG
  CTGTCCCTTCATTGAATTTTAGAATGATTGAAGATAGTGGGAAAAGAGGAAATACCATGG
  CAGAAAGAAGACAGCTGTTTGCAGAGATGAGGGCTCAAGATCTGGATCGCATCCGACTCT
  CCACCTACAGAACAGCATGCAAGCTTAGGTTTGTTCAGAAGAAATGCAATTTGCACCTGG
  TGGACATATGGAATGTCATAGAAGCATTGCGGGAAAATGCTCTGAACAACCTGGACCCAA
  ACACTGAACTCAACGTGTCCCGCTTAGAGGCTGTGCTCTCCACTATTTTTTACCAGCTCA
  ACAAACGGATGCCAACCACTCACCAAATCCATGTGGAGCAGTCCATCAGCCTCCTCCTTA
  ACTTCCTGCTTGCAGCGTTTGATCCGGAAGGCCATGGTAAAATTCAGTATTTGCTGTCAA
  AATGGCTTTAGCCACATTGTGTGGAAGGAAGATCATGCACAAATTAAGATATATTTTCTC
  AATGGATTCTGACTCCAGTGGGGTGATGGGTTATGGACGATATGACCAATTCCTTCGGGA
  GNTCTCAAACTACCCACGCAGTNTNC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032978 unedited
  CCCCCCGGCCCNNCCCCCNNCNNNCCCCNAAAAAGTTTGACTTTGNACCGCGCNGCCGCA
  TCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGTTTGCTGCTCCCCCTGCTGGCTCTTGA
  GCCTGACCACAGGTTCAGACAGCTCCAGTTTAGGCTATAAAGTCACAATGCCCCTATTTC
  CAGAAAAAAAAAAAGTATGTAATGACCCTCTCGTCTCCCGGAGCCGAAAGGCTCTGGTGC
  TAAGGAAAAAGCTGAGAGTTACTTTTTAAAAAGAGATTACTGTCAACCAACAGTAAGGAT
  TCTGCTTTGGGGAAGAGCCAAAAAGATGGGACGTGAATAGTCAGTTATGGAGTACAGTCT
  TTCTCTCACAAAATTGAGCACATTTTGGTGAGCCATGGGGAACAAGGCTCAATAATTGTG
  TGATGAAAAGGGATTTAATTGCCTGATTTTTATAAAAAGAGGTTTCACAGGTTTTCAGTA
  AAATGAAACCGAACTGAATACTCACAGTGTGAGTGGGTGTAGAGGTTAGCTTCTGAGTGC
  TGTGGGTTTTGGGATGACATGAACCTCAGGGTATCTCTAGTGACTGTGGTATCTCTCTGT
  TAAGAGAAGGGTCTGATCAACAACTTGAAAAGCTAAGACTGGTTTGAAACTTATACCTCT
  GGGGATCTAAAATGTTTCCAATAAATGACAATTCGTTCCCTTTTCTAAAAGATGTCATAG
  NTTTTCTTTTGATGTGGATAGACGTGATAGTTATAAAAAATGCTAAATTTATATATCTCA
  ATTCCAGCCCTACTTTGAAAAAGAAGCACAATTATATGACTTTTTTCTCTTTTCCAAACT
  GAGCCTGAACATACCTTAAAGCCAAAGCAATGAAAAAACCCGCGGGCGGCAGGCCCCTCC
  CCTGTTAATTAAGAACCGCCATAGGGGAACCTAACTACTCCCTGCTTCAGTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032978
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032978.4, NP_116760.2
• RefSeq Size: 3123 bp
• RefSeq ORF: 1704 bp
• Locus ID: 1837
• Cytogenetics: 18q12.1
• Domains: ZnF_ZZ
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4), also known as beta transcript, lacks an internal coding exon and multiple exons from the 3' end, and contains an alternate 3' exon, compared to transcript variant 1. This results in a shorter isoform (4) with a distinct C-terminus and missing an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.