Cytochrome b5 (CYB5A) (AK026310) Human Untagged Clone

CAT#: SC102219

(untagged)-Human cDNA: FLJ22657 fis, clone HSI07791, highly similar to HUMCYB5 Human cytochrome b5 mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYB5A
• Synonyms: CYB5|MCB5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK026310, the custom clone sequence may differ by one or more nucleotides
  
  
  GAGTCGCGCGCTCTGCTCCACCCGACGGGGCTGTGTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGC
  AGAGCAGTCGGACGAGGCCGTGAAGTACTACACCCTAGAGGAGATTCAGAAGCACAACCACAGCAAGAGC
  ACCTGGCTGATCCTGCACCACAAGGTGTACGATTTGACCAAATTTCTGGAAGAGCATCCTGGTGGGGAAG
  AAGTTTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAACTTTGAGGATGTCGGGCACTCTACAGATGC
  CAGGGAAATGTCCAAAACATTCATCATTGGGGAGCTCCATCCAGATGACAGACCAAAGTTAAACAAGCCT
  CCGGAAACTCTTATCACTACTATTGATTCTAGTTCCAGTTGGTGGACCAACTGGGTGATCCCTGCCATCT
  CTGCAGTGGCCGTCGCCTTGATGTATCGCCTATACATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGC
  GCAGGAAGAGCCTGCTTTGGACACGGGAGAAAAGAAGCCATTGCTAACTACTTCAACTGACAGAAACCTT
  CACTTGAAAACAATGATTTTAATATATCTCTTTCTTTTTCTTCCGACATTAGAAACAAAACAAAAAGAAC
  TGTCCTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTATTCATCTACTTTATTTTGATGTTTCCTTAA
  TGTGTAATTTACTTATTATAAGCACGATCTTTTAAAAATATATTTGGCTTTTAAAGTAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK026310 unedited
  AGGGGGCATATTAGGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGCGC
  GCTCTGCTCCACCGAGGGGCTGTGTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGCA
  GAGCAGTCGGACGAGGCCGTGAAGTACTACACCCTAGAGGAGATTCAGAAGCACAACCAC
  AGCAAGAGCACCTGGCTGATCCTGCACCACAAGGTGTACGATTTGACCAAATTTCTGGAA
  GAGCATCCTGGTGGGGAAGAAGTTTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAAC
  TTTGAGGATGTCGGGCACTCTACAGATGCCAGGGAAATGTCCAAAACATTCATCATTGGG
  GAGCTCCATCCAGATGACAGACCAAAGTTAAACAAGCCTCCGGAAACTCTTATCACTACT
  ATTGATTCTAGTTCCAGTTGGTGGACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGCC
  GTCGCCTTGATGTATCGCCTATACATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGCG
  CAGGAAGAGCCTGCTTTGGACACGGGAGAAAAGAAGCCATTGCTAACTACTTTAACTGAC
  AGAAACCTTCACTTGAAAACAATGATTCTAATATATCTCTTTCTTTTTCTTCCGACATTA
  GCAACAAAACAAAAAGAACTGTCCTTTCTGCGCTCAAATTTTTTGAGTGTGCCTTTTTAT
  TAATCTACTCTATTTTTGATGGTTTCCTTTATGCGGAAATTACTTATTATAAGCCATGAC
  CTTTAAAAAAATTATTGGGCTTTTAAATCCANNAAAAATTTTCTATACCATTCCTCCACT
  AAACTCATATACAANTTAAACAACAAAAATAACCTCTCCTCTTTAATGGGCGGCGCGGTC
  TATTTGTTTTCTTCAAAAAAACCGCGGGAATCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK026310 unedited
  ATCTAGAGTCGAGTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTAACTTTAAAACCCAAATTTTTTTTTAAAAAAACTTGCTTTT
  AATAAGTAAATTCCCCTTTAGGGAAACTTCAAAATAAAGTTATTAAATAAAAGGGCCCCC
  TTTAAAAATTTTGACCCCAAAAAGGACAGTTTTTTTTGTTTTGTTTCAAAGGGGGGAAAA
  AAAAAAAAAAAATTTTTTAAATTTTTTTTTTTAAAAAAAAGGTTTTTTCCATTTAAAATA
  TTTAAAATTGGTTTTTTTTTTCCCGGGCCCAAAAAAGGTTTTTTTTGCCTTAATTTTTGA
  GGAGGGGTTCACCCCTTTGCCTTTTTTAGGCAAACCTTAAAGGCGACGGCCACTTCAAAA
  ATGGCAGGGATCCCCCCGTTGGGCCCCCACTTGGAACTAAAATCAATAGTGGGGATAAAA
  ATTTCCGGAGGCTTGTTAAACTTTGGGCTGCCCTCGGGAGGGACCTCCCCAAAGAAAAAG
  GTTTTGAACTTTTCCCTGCCTTTGGAAAAGGGCCCAATTCTCTAAATTTTTAGTAGGTAC
  CCTCCATTTGTTCCTTAAAATTTTTTCCCCCAGAAAGTTTTTCAAAATTTGGCAAATCAA
  CCCTTTTGGGGAAAAACACCACGGGTTTTTGTGGGTTGGTTTTTAAATCCCTTTAGGGGG
  GAAATTTAGGCCTTTTTCACTGTTTCCATTCTGGTCGCACGGACCGTCCCAACAAAAATT
  TCCTTGGGGGAAAAAAACCCGCCCTTGCCAATTTTGGGCCCCTTAAGGAGTTTTTTCAAT
  TTTGCGGTCCCAAAAACACTTTTATTTTACTCTCCCCGCCCCCACCCCCTTTGGTCCGCG
  GGGGTTTTTCCACTTTGGAATCCCCTTTTTTTCGAAAAACATCCTTGCTACGGGGGGACC
  TTAATCCTCGCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: AK026310
• Insert Size: 840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK026310.1
• RefSeq Size: 784 bp
• RefSeq ORF: 784 bp
• Locus ID: 1528
• Cytogenetics: 18q22.3
• Domains: heme_1
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'