MAP3K4 (AK094629) Human Untagged Clone

CAT#: SC103206

(untagged)-Human cDNA FLJ37310 fis, clone BRAMY2016706

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC103206 sequence for AK094629 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCAGGCGTGACGGCAAAGGCGGGAGGAGGGACCAGCGCGGCAGCGGCTTCT
  CTCATCCGTGCACGGAGCCCCGCATGGCCGCGGCGGGCAGTAAGCTGCAGCCTGGCGCGG
  GGCACCGGAGCCCCGAAGTGCGGGAGTGACTCGACTCCGCCTCCGCCTCTACCCCGCCGC
  AGGAGCCGTGCGCGCCGCGGCCATCTTGGAAACGGCGACCGGCCGAGCGCCGGGGCTACG
  GTGGGCGGCGAGGGACAAGCCTCCCAGATCGGCGGCGGTGGCGGCGGCGGCGGCGGGCGC
  CGGAATGCGACCGGACGGGGGCGGAGACGGCGGCAGGGAGGGGCGGGGAGCGCCGTCAAG
  TGTCGGCGCGATTCGGTCCTGGAGCCGGGTCTCGCCAGCTCGCCCGGTGTCGCCCCGGCC
  GGTGGCAGTGGGGGACTGTGGAGCGGAGCCGGGCTGTGCAGCGGCCTGGGAGCTCGAGGC
  TTCCCAGGCGGTCGTCGGTCTGGGAGGGACGCTGGGTAG
  
  Clone variation with respect to AK094629.1
  465 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK094629 unedited
  GGGGTCAGAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCGTTGA
  GGGTGAGCTGGGGGAGTCTACAAGGGAAGGGGAGCCGGCAACACGCTCGAGCGGAACCCT
  GCGGAGCTGCAACCCCGGGAGGAGTCGCCGGACAGACTCAGACGCGGTCCCCTGGGACCC
  AGGGATGCACCGCGCCCCTCCCGCCCCAGCCTGCAGAGACTGGATGCTTCCCTCCCCACC
  TGGCGGGAAGCAGAGGCCCTCGGGCCCGACCGAGTTCGGGCCAGGACCCGCCCCGCTTTC
  TGCGAGCGACCGACTGCGGCCGCCACACTCACCTCGCCGCCAAGCAGCACTCGGGTTCTG
  ACTCGGTCTCGGGTTCCGGTGGCGGTGGTGGCGGCGGCGGCGGTGGCGGCGGCTCCTCCA
  TGGCGGCGGCAGGCGTGACGGCAAAGGCGGGAGGAGGGACCAGCGCGGCAGCGGCTTCTC
  TCATCCGTGCACGGAGCCCCGCATGGCCGCGGCGGGCAGTAAGCTGCAGCCTGGCGCGGG
  GCACCGGAGCCCCGAAGTGCGGGAGTGACTCGACTCCGCCTCCGCCTCTACCCCGCCGCA
  GGAGCCGTGCGCGCCGCGGCCATCTTGGAAACGGCGACCGGCCGAGCGCCGGGGCTACGG
  TGGGCGGCGAGGGACAAAGCCTCCCAGATCGCGGCGGTGGCGGCGGCGGCNGCGGGCGCC
  GGAATGCGACCGGACGGNGGCNGANAACGCNGCANGGANGGGGCGGNGAAGCGCCNTCAA
  GTGTCGGCGCGATTCGGTCCTGGACCCGGTCTCGNCAGCTCGNCCGGTGTCNCCCNGNCC
  GTGGCANTGGGGACTGTGGAGCNNGACGGGCTGTGCANCGGCCTGGGAGCTCNAGCTTTC
  CCAGCGTCTTCGTCCTGGAGGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK094629 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAGAT
  TTATTTTATTATTTAACTTTTAAAGTCTCTAATATGCCTTCTAAGAAGGGTTCTGGCTCA
  GAAAATTGCAGAAGAAAATCGAGTAGAGAACTAGAATTACAGAAACCTAAGAAATACTTT
  TACTGAGCTTTCTTCACACAAGAAAATGCACGGAAATGAATTATACATGGATACCAATTT
  GTGATTTTTAAAAATTTGTAATTTTTTAAATTAAGATAAAAATTAGTTTATTTTTTTGAA
  TACTTTTTTGTTTTGTTTTACCTCACCCTTTACTAAGCTGTTAGCTTGGTCTGTTGCGCT
  TACTGCTATATCCTCAGCATCTAGTACAGTTCCTGACACATATATGCACTTAATAAGTAT
  TTGTCAAATGAATATTATTGAGTAAATAAGATGAAGGTCAATTCAGAAATTCAGCACTAT
  CAGGCTATATACATAATACAGACTATTATAAAAAACTAGAATGGAGCAGTTCCTTTTAAC
  GACGGAGAAATGTCAGTAACATACTTTGAGCACAATATAGCTAATTTCACAACTGGTCTT
  TAAATATAACATACCTATAATCAATTTAATGTTTTTCTTTGTTACCAGAGTTGTTCAAAA
  CGTGCGTTTTACCTATCAAAGCTCAACTAAATGAGGTTTAAGAAATCTGCACATTTGAAT
  GTACTTAATTTTAAAAGATTCTGGTTTTGGATTAACTCTGGATTTCACCCTATGGGAAAT
  TTTGTAATATTTCCAACTTAAAAAAACTGTGAAGCCCAGCGCTAGAGTAACGATGCATTG
  GATTTCACAGGATTCTGTACTCTGAATATCCTAGCATTTCTCTTCCCCATCCCTCGGGTA
  GAGCATCAGTCCAGTTCTTGGAATCGTTCGATCTAGACCTGGTGAGAGTTTGCGGNTGAA
  TGAGGGTCCTTAAATCGGATCCGAACTTTGTCGCTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: AK094629
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK094629.1, BAC04390.1
• RefSeq Size: 2028 bp
• RefSeq ORF: 2028 bp
• Locus ID: 4216
• Cytogenetics: 6q26
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: GnRH signaling pathway, MAPK signaling pathway
• Gene Summary: 'The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]'