alpha Defensin 1 (DEFA1) (BC027917) Human Untagged Clone

CAT#: SC104065

DEFA3 (untagged)-Homo sapiens, defensin, alpha 3, neutrophil-specific, clone MGC:34189 IMAGE:5226993, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEFA3
• Synonyms: DEF3; defensin, alpha 3; defensin, alpha 3, neutrophil-specific; defensin 3, neutrophil-specific; HNP-3; HNP3; HP-3; neutrophil peptide 3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC027917, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCTGAGCCACTCCAGG
  CAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCAGAAGTGGTTGTTTCCCT
  TGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGAAAAACATGGACTGCTATTGCAGAATA
  CCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCT
  GCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027917 unedited
  GATTTTGTAAACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGCTGGGACAGAGGA
  CTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGA
  GGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCTGAGCCAC
  TCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGCGGACATCCCAG
  AAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAGCATCCAGGCTCAAGGA
  AAAACATGGACTGCTATTGCAGAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAA
  CCTGCATCTACCAGGGAAGACTCTGGGCATTCTGCTGCTGAGCTTGCAGAAAAAGAAAAA
  TGAGCTCAAAATTTGCTTTGAGAGCTACAGGGAATTGCTATTACTCCTGTACCTTCTGCT
  CAATTTCCTTTCCTCATCTCAAATAAATGCCTTGTTACAAGAAAAAAAAAAAAAAAAAAA
  ACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCAT
  CCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACC
  AGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATA
  TTATGGGGTGGAGGGGGGTGGTATGGAGCAGGGNGCAAGTTGGGAAGACAACCTGTANNG
  GCCTGCGGGTCTATTGGGAACAAGCTGNAGTGCAGTGGCACAAATCTGGCTCACTGNCAT
  CTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCACCTNCCGAGTTGTTGGGATTCCAG
  CATGCATGACCAGNCTCAGCTAANTTTTGTTTTTTGGGAGAGACGGGGTNTCACATATGG
  GCCAGCTGGNCTCCACTCCTAATCTCAGTGATCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC027917 unedited
  NNNGGGTTTCACTATGGAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTTCTTGTAACAAGGCATTTATTTGAGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGA
  GTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCA
  AGCTCAGCAGCAGAATGCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTC
  TCCTGCAATGCACGCTGGTATTCTGCAATAGCAGTCCATGTTTTTCCTTGAGCCTGGATG
  CTTTGGAGCCAAGCTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGATGTCCGCTGC
  AATCTGCTCCGGGGCTGCAGCAACCTCATCAGCTCTTGCCTGGAGTGGCTCAGCCTGGGC
  CTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGTCCTCATGGCTGGGGTCAC
  AGATCCTCTAGCTAGGCAGGGTGACCAGAGAGGGCAGACAGCAGTCCTCTGTCCCAGCTC
  GTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAA
  ACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGG
  GGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCC
  ATTGACGTCAATGGGGTGGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCC
  CATTGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGT
  ACTGGCCAGTAGGAAAGTCCCGTAGGGTCATGTACTGGGCATAATGCCCAGCGGGCCATT
  TACCGTCAN
  
• Restriction Sites: NotI-NotI
• ACCN: BC027917
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027917.1, AAH27917.1
• RefSeq Size: 556 bp
• RefSeq ORF: 285 bp
• Locus ID: 1668
• Cytogenetics: 8p23.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation. [provided by RefSeq, Oct 2014]'