FGF14 (NM_175929) Human Untagged Clone

CAT#: SC106966

FGF14 (untagged)-Human fibroblast growth factor 14 (FGF14), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGF14
• Synonyms: FGF-14; FHF-4; FHF4; SCA27
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106966 sequence for NM_175929 edited (data generated by NextGen Sequencing)
  ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
  AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
  TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
  AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
  TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
  GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
  ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
  TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
  ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
  AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
  TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
  GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
  AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACATAG
  
  Clone variation with respect to NM_175929.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175929 unedited
  ATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTGCATTACTGT
  TCTCCCTGATTAGTTTTGATCTCAGTTGGGATCTCTTTGCTTTTCTGTTTGGCTTCATGC
  TGAAAAAGGATTTTTTCTCCAACCCTTTGGTAATAATCCGGTGGTGATCGAGGGGGGATA
  AATCATTCACCCTGGCCGAAAACAAACAATCACTGAGAAGTCTCAAAGAAATATACCACG
  TGAGGGGAAAAAACTGGGAGAAGATCCGGAATATTATCGTTTTTCCTATGGTAAAACCGG
  TGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCACAAGGATCTCTTCT
  TTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATGTGGTTTCTTTGGA
  ACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAGAGTCTTAAGAAAA
  ACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTATATTGCAGGCAAG
  GCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAGGATGACAGCACTA
  ATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCCATCCAGGGAGTGA
  AAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCATCAGAACTTTTTA
  CCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATATTATGTAATCTACTCATCCAT
  GTTGTACAGACACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTAAATAAGGAAGGNNCA
  GCTATGANAGGGAACAGAGTANAGAAAACANACCAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175929
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175929.1, NP_787125.1
• RefSeq Size: 1001 bp
• RefSeq ORF: 759 bp
• Locus ID: 2259
• Cytogenetics: 13q33.1
• Protein Families: Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A mutation in this gene is associated with autosomal dominant cerebral ataxia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has an alternate 5' sequence including the 5' UTR and coding region, as compared to variant 1. It encodes isoform 1B, which has a different and longer N-terminus than isoform 1A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.