BSCL2 (NM_032667) Human Untagged Clone

CAT#: SC108137

BSCL2 (untagged)-Human Berardinelli-Seip congenital lipodystrophy 2 (seipin) (BSCL2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BSCL2
• Synonyms: GNG3LG; HMN5; PELD; SPG17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108137 sequence for NM_032667 edited (data generated by NextGen Sequencing)
  ATGGTCAACGACCCTCCAGTACCTGCCTTACTGTGGGCCCAGGAGGTGGGCCAAGTCTTG
  GCAGGCCGTGCCCGCAGGCTGCTGCTGCAGTTTGGGGTGCTCTTCTGCACCATCCTCCTT
  TTGCTCTGGGTGTCTGTCTTCCTCTATGGCTCCTTCTACTATTCCTATATGCCGACAGTC
  AGCCACCTCAGCCCTGTGCATTTCTACTACAGGACCGACTGTGATTCCTCCACCACCTCA
  CTCTGCTCCTTCCCTGTTGCCAATGTCTCGCTGACTAAGGGTGGACGTGATCGGGTGCTG
  ATGTATGGACAGCCGTATCGTGTTACCTTAGAGCTTGAGCTGCCAGAGTCCCCTGTGAAT
  CAAGATTTGGGCATGTTCTTGGTCACCATTTCCTGCTACACCAGAGGTGGCCGAATCATC
  TCCACTTCTTCGCGTTCGGTGATGCTGCATTACCGCTCAGACCTGCTCCAGATGCTGGAC
  ACACTGGTCTTCTCTAGCCTCCTGCTATTTGGCTTTGCAGAGCAGAAGCAGCTGCTGGAG
  GTGGAACTCTACGCAGACTATAGAGAGAACTCGTACGTGCCGACCACTGGAGCGATCATT
  GAGATCCACAGCAAGCGCATCCAGCTGTATGGAGCCTACCTCCGCATCCACGCGCACTTC
  ACTGGGCTCAGATACCTGCTATACAACTTCCCGATGACCTGCGCCTTCATAGGTGTTGCC
  AGCAACTTCACCTTCCTCAGCGTCATCGTGCTCTTCAGCTACATGCAGTGGGTGTGGGGG
  GGCATCTGGCCCCGACACCGCTTCTCTTTGCAGGTTAACATCCGAAAAAGAGACAATTCC
  CGGAAGGAAGTCCAACGAAGGATCTCTGCTCATCAGCCAGGGCCTGAAGGCCAGGAGGAG
  TCAACTCCGCAATCAGATGTTACAGAGGATGGTGAGAGCCCTGAAGATCCCTCAGGGACA
  GAGGGTCAGCTGTCCGAGGAGGAGAAACCAGATCAGCAGCCCCTGAGCGGAGAAGAGGAG
  CTAGAGCCTGAGGCCAGTGATGGTTCAGGCTCCTGGGAAGATGCAGCTTTGCTGACGGAG
  GCCAACCTGCCTGCTCCTGCTCCTGCTTCTGCTTCTGCCCCTGTCCTAGAGACTCTGGGC
  AGCTCTGAACCTGCTGGGGGTGCTCTCCGACAGCGCCCCACCTGCTCTAGTTCCTGA
  
  Clone variation with respect to NM_032667.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032667 unedited
  GTCGCAATTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCACGAGGTGAATCTTCG
  TTTCTCTCCCAGGACCCTCCATTTTCCATATCCAGGAAAATGTGATGCGCCACAGGTATC
  AGCGTCTGGATCGCCACTTCACGTTTTAGCCACAAGTGACTCAGTGGAAGATCCAGAGTC
  AACAGAGGCTCGTCAGGAAGATGTCTACAGAAAAGGTAGACCAAAAGGAGGAAGCTGGGG
  AAAAAGAGGTGTGCGGAGACCAGATCAAAGGACCGGACAAAGAGGAGGAACCACCAGCTG
  CTGCATCCCATGGCCAGGGGTGGCGTCCAGGTGGCAGAGCAGCTAGGAACGCAAGGCCTG
  AACCTGGGGCCAGACACCCTGCTCTCCCGGCCATGGTCAACGACCCTCCAGTACCTGCCT
  TACTGTGGGCCCAGGAGGTGGGCCAAGTCTTGGCAGGCCGTGCCCGCAGGCTGCTGCTGC
  AGTTTGGGGTGCTCTTCTGCACCATCCTCCTTTTGCTCTGGGTGTCTGTCTTCCTCTATG
  GCTCCTTCTACTATTCCTATATGCCGACAGTCAGCCACCTCAGCCCTGTGCATTTCTACT
  ACAGGACCGACTGTGATTCCTCCACCACCTCACTCTGCTCCTTCCCTGTTGCCAATGTCT
  CGCTGACTAAAGGTGGACGTGATNCGGTGCTGATGTATGGACAGCCGTATCGTGTTACCT
  TAGAGGCTGAGCTGCCAGAGTCCCCTGTGAATCAAGATTTGGGCATGGTTCTGGGTCACC
  TTCCCTGCTACACCAGAGGTGGGCGAATCATCTCCACTTCTTCGCGTTCCGTGATGCTGC
  ATTACCGGTCAGACTTGTTCCAGATGCCTGAAACAACTGGTTCTCCTCTAGGCTCCTGCT
  ATTTGGGCTTTGCAAAACAAAAGCAGCTGCTGGAAGTGGGAACTTTACCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032667 unedited
  GAACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGCTGACCCAAAATAGTTTATTGAAGG
  AAAAACCAGGGGAAAGGAGTCAGGGGGGAAAATGCTGGAATGGGAGGAATCTGCCCCTTT
  TTTTTAAGAACTAAAACAGGGGGGGCGCTTTCGGAAAACACCCCCAACAGGGTCAAAACT
  GCCCAAAATCTTTAAGACAGGGGCCAAAACCAAAACCGGAGCCGGAGCCGGCCGGTTGGC
  CCCCCTCAAAAAAACTGGATTTTCCCAAGAACCTGAACCATCACTGGCCTCAGGGTCTAA
  CTCCTTTTTTCCCCTCAAGGGCTGGTGAACTGGGTTCTCCTCCTCGGACAAGTGACCCTT
  TGGCCCTGAGGGAACTTCAGGGCTCTCACCATCCTCTGTAACATCTGATTGGGGAGGTGA
  CTCCTCCTGGCCTTTAAGCCCTGGCTGATGAACAAAAAACCTTCGTTGGACTTTCTTCCG
  GGAATTGGCTTTTTTTCGGATGGTAACCCGCAAAAAAAAACGGGGTCGGGGCCACATGCC
  CCCCCACACCCACTGGATGTATCTGAAAAACACCATGACCCTTAAGAAAGTGAAATTGCT
  GGCGACACCTCATGAACGCCCAAGTCACTCGGGAATTGATAACAGGTTTCTGAACCCACC
  GAATGCCCCCGGGAGCCGGAGTAAGCTCCATACACCTGGATGGCGCTTGTTGGGGATCTT
  AAAGAACCCCTCCAACGGGTCGGGACCCAACAAATTTTTTCTATAGATTGGGGAGAAGTC
  CCCCCTCCCCACCTTGTTCTGTGCTTTGTAAACCCAATAAGGGGGGGGTAAAAAAAAACC
  TGGGGCCCCACATTGGGACCGGCTCTTGGGGTTTCGCCCCTCCCTACCCCAAAACGGGAT
  ATAATCCCCCACCTTGGGGCCAGGAAAGGGGCCCAAACTCCCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032667
• ORF Size: 1197 bp
• Insert Size: 1600
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_032667.4, NP_116056.3
• RefSeq Size: 1664
• RefSeq ORF: 1197
• Locus ID: 26580
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes the multi-pass transmembrane protein protein seipin. This protein localizes to the endoplasmic reticulum and may be important for lipid droplet morphology. Mutations in this gene have been associated with congenital generalized lipodystrophy type 2 or Berardinelli-Seip syndrome, a rare autosomal recessive disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HNRNPUL2 (heterogeneous nuclear ribonucleoprotein U-like 2). [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1.