MRPL43 (NM_032112) Human Untagged Clone

CAT#: SC108410

MRPL43 (untagged)-Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL43
• Synonyms: bMRP36a; L43mt; MRP-L43
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108410 sequence for NM_032112 edited (data generated by NextGen Sequencing)
  ATGACGGCGCGCGGGACTCCGAGCCGCTTCTTGGCCAGCGTTCTCCACAACGGACTGGGT
  CGCTATGTGCAGCAGCTGCAGCGTCTGAGCTTCAGCGTCAGCCGCGACGGCGCCTCGTCT
  CGCGGCGCCAGGGAGTTCGTGGAGCGGGAGGTGATCGACTTCGCCCGACGGAATCCAGGG
  GTCGTAATATATGTAAACTCGCGTCCGTGCTGCGTGCCCAGAGTAGTGGCCGAATACCTT
  AACGGGGCTGTGCGCGAGGAGAGCATCCACTGCAAGTCGGTCGAGGAGATCTCGACGCTG
  GTGCAGAAGCTGGCCGACCAGTCGGGCTTGGACGTGATCCGCATCCGCAAGCCCTTCCAC
  ACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCACCAACAAGCCGACCACGTTC
  CGCGGGCTACGCCCCCGAGAGGTTCAGGATCCTGCCCCAGCCCAGGTGCAAGCACAGTGA
  
  Clone variation with respect to NM_032112.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032112 unedited
  GTTAGNATTTGTATCCGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTAGGCTCC
  GCGGCCTCCAAGCTGTAGCTATGACGGCGCGCGGGACTCCGAGCCGCTTCTTGGCCAGCG
  TTCTCCACAACGGACTGGGTCGCTATGTGCAGCAGCTGCAGCGTCTGAGCTTCAGCGTCA
  GCCGCGACGGCGCCTCGTCTCGCGGCGCCAGGGAGTTCGTGGAGCGGGAGGTGATCGACT
  TCGCCCGACGGAATCCAGGGGTCGTAATATATGTAAACTCGCGTCCGTGCTGCGTGCCCA
  GAGTAGTGGCCGAATACCTTAACGGGGCTGTGCGCGAGGAGAGCATCCACTGCAAGTCGG
  TCGAGGAGATCTCGACGCTGGTGCAGAAGCTGGCCGACCAGTCGGGCTTGGACGTGATCC
  GCATCCGCAAGCCCTTCCACACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCA
  CCAACAAGCCGACCACGTTCCGCGGGCTACGCCCCCGAGAGGTTCAGGATCCTGCCCCAG
  CCCAGGTGCAAGCACAGTGAAGAGTTGCCCCACCAACTGCAGCCCCAGGCTTTGGACTGT
  TACTCCGGTAAAGGTGGTTCTTCCCCTTTGGGATTCCAAGCCCAGGCAAATGGAACCCAT
  CAATGGGCAAGTTGACAGAGGTTCTGCTTGGGATAATGAAGAGCTGCCTGTTTCTTTCCA
  GTGCCTGCTTCTGGGGGCAGTGACCTTGTGAACCACTCATTNTTATGCAAGTGGCATCCC
  TAAACCTGAGATGAGGAGACTTCAAGGGTTTTACGGACCCTTGTTTTTAATCCANNATGA
  TATATGATCTCNAAACANTGAGAGGTCTGAAGGCTGGCTCTGAGAATCCCTGATGTCTAT
  TGGACACCCTGACTACGAAGCTTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032112 unedited
  AAAACCAAAGAAAAAAAAANNCNCCCCNNCCCCCCNTANTTTTGACTTTGANCCGCGGNC
  CGCATNCNAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTGGCCTTGGGTAAGTTTATTAA
  CCTGTTTTATAAATCTGTATTCACACAGATATAAAGTGCCTAGCCCATCACAGCAGAGCT
  CTCCGTAGCTCAGTGGTTGTTCCAATAAGACATCAGGGATTCTTCAGAAGCCAGCCTTCA
  GACCTCTCACTGTGTTTTGAGATCATTATTATCAATTTGGATTTAAAAAACAAGGGTCCT
  GTAAAACCCTTGAAGTCTTCCTCATCTCAGGTTTTAGGGATGCCACTTGCATAAAAATGA
  GTGGTTCACAAGGTCACTGCCCCCAGAAGCAGGCACTGGAAAGAAACAGGCAGCTCTTCA
  TTATCCCAAGCAGAACCTCTGTCAACTTGCCCATTGATGGGTTCCATTTGCCTGGGCTTG
  GAATCCCAAAGGGGAAGAACCACCTTTACCGGAGTAACAGCCCAAAGCCTGGGGCTGCAG
  TTGGTGGGGCAACTCTTCACTGTGCTTGCACCTGGGCTGGGGCAGGATCCTGAACCTCTC
  GGGGGCGTATTCCGCGGAACGTGGGCGGCTTGTTGGTGAAAGGGTGCCACTGGCCCTGAA
  TGCCTAGGTTGTCGGTGTGGAAGGGCTTGCGGTTGCCGATTACGTTCAACCCTGACTGGT
  CCGCCCTCTTCCTGCACCAGCTGTCAATACCCCCTCGACCGACTCCGCCTGGATGCCTCT
  CCCTTGTCCCCCACCCCCCCTCAGGTGTTTCGGCCCCTTATTTTGGGTCCCCCCTCCGGT
  CTCCAAGTTCCATAACTTACCTCCCCCCGGGTATCCCTGGGGGAAATCCGACAACCCCAC
  CCTCCCCAACTTTTTGCTCCACTCACACTAGCCCCCTCCTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032112
• ORF Size: 480 bp
• Insert Size: 1000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_032112.2, NP_115488.2
• RefSeq Size: 1016
• RefSeq ORF: 480
• Locus ID: 84545
• Domains: L51_S25_CI-B8
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform a.