PRKACB (NM_002731) Human Untagged Clone

CAT#: SC108499

PRKACB (untagged)-Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKACB
• Synonyms: PKA C-beta; PKACB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002731 edited
  ATGGGGAACGCGGCGACCGCCAAGAAAGGCAGCGAGGTGGAGAGCGTGAAAGAGTTTCTA
  GCCAAAGCCAAAGAAGACTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCGGA
  CTTGAAGATTTTGAAAGGAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCATGTTG
  GTAAAACACAAAGCCACTGAACAGTATTATGCCATGAAGATCTTAGATAAGCAGAAGGTT
  GTTAAACTGAAGCAAATAGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGAAT
  TTTCCTTTCCTTGTTCGACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGGTT
  ATGGAATATGTCCCTGGGGGTGAAATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGT
  GAGCCCCATGCACGGTTCTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATTCA
  CTAGACCTCATCTACAGAGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCTAT
  ATCCAGGTCACAGACTTTGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTATGT
  GGAACTCCAGAGTATTTGGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAGTG
  GATTGGTGGGCATTAGGAGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCTTT
  GCAGACCAACCAATTCAGATTTATGAAAAGATTGTTTCTGGAAAGGTCCGATTCCCATCC
  CACTTCAGTTCAGATCTCAAGGACCTTCTACGGAACCTGCTGCAGGTGGATTTGACCAAG
  AGATTTGGAAATCTAAAGAATGGTGTCAGTGATATAAAAACTCACAAGTGGTTTGCCACG
  ACAGATTGGATTGCTATTTACCAGAGGAAGGTTGAAGCTCCATTCATACCAAAGTTTAGA
  GGCTCTGGAGATACCAGCAACTTTGATGACTATGAAGAAGAAGATATCCGTGTCTCTATA
  ACAGAAAAATGTGCAAAAGAATTTGGTGAATTTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002731 unedited
  GTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCTTCCCTTCCCTGACCC
  CTTCTTGCCATCGCCCCAGACTGGGGAACGCGGCGACCGCCAAGAAAGGCAGCGAGGTGG
  AGAGCGTGAAAGAGTTTCTAGCCAAAGCCAAAGAAGACTTTTTGAAAAAATGGGAGAATC
  CAACTCAGAATAATGCCGGACTTGAAGATTTTGAAAGGAAAAAAACCCTTGGAACAGGTT
  CATTTGGAAGAGTCATGTTGGTAAAACACAAAGCCACTGAACAGTATTATGCCATGAAGA
  TCTTAGATAAGCAGAAGGTTGTTAAACTGAAGCAAATAGAGCATACTTTGAATGAGAAAA
  GAATATTACAGGCAGTGAATTTTCCTTTCCTTGTTCGACTGGAGTATGCTTTTAAGGATA
  ATTCTAATTTATACATGGTTATGGAATATGTCCCTGGGGGTGAAATGTTTTCACATCTAA
  GAAGAATTGGAAGGTTCAGTGAGCCCCATGCACGGTTCTATGCAGCTCAGATAGTGCTAA
  CATTCGAGTACCTCCATTCACTAGACCTCATCTACAGAGATCTAAAACCTGAAAATCTCT
  TAATTGACCATCAAGGCTATATCCAGGTCACAGACTTTGGGTTTGCCAAAAGAGTTAAAG
  GCAGAACTTGGACATTATGTGGAACTCCAGAGTATTTGGCTCCAGAAATAAATTCTCAGC
  AGGGCTACAATAAGGCAGTGGATTGGTGGGCATTANGAGTGCTAATCTATGAAATGGCAG
  CTGGCTATCCCCCATTCTTTGCAGACCAACCAATTCAGATTATGAAAAGATTGNTCTGGA
  AAGGTCCGATCCCATCCACTTCATTCAGATCTCAGGACTTCTACGNACTGCTGCAGGTGG
  ATTGACAAGAATTNGAATCTAGGAAGGTGGCAGGATATAAACCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002731 unedited
  NNNNTTTTTTTNNTNNNNNNNNNTTTTTNNNTNTNNNNTTTTTTTTTTTTTTTTTTTTAA
  AATATTTTATACGAATGATGTTTTAAATTTATTAACATGACGATATGCCTTTGTTACACA
  AAACTTGCTAGAGCAGAGTATTTTATTTTTTTCTTGCACGTTTTAAATATAGATGGACCA
  GTGAAATCAATATCAGCATTTACATGACATTACCATGCAGAAACACTTGATAACCGAAAG
  ACATATAGATTGGTAGAAAGAACATCTCAAACAGGTTTTTTTTATGTCTTCAAAGGTTTC
  ACCAGGGTGTGGTACAGATGAAAAGAAGTGGTGTTAATGACCTACCTGCGCCGATAATAA
  AGCAAATAGAAGGATTATATACATTAAGCATCAGCTTGACTAAAAATAAACTCTAAATGC
  AGGTAAATTGATCATTAGAATGAACCCAGTTTATCTCTTCTCGTGTGTTGTTTTAAGGTA
  GCTCGCTGGACCCCAGAGTTTATATCGAATGGTAAATATTACCGCCCGGCGAAGCAACGC
  AACCAGTGGGCTCAACGCTGGGTTCCTAAGCAATACGTAACCAGATACTCTGAAGCGGCC
  GGGATTTGCACACGCCGGCGAGACTTCGAGTCGCGTGCGAAAAAGCCCGCGACGGAAGCC
  CCACGGGCGCGGAGCTGGTGGGTTGCGGGGGAACGAGGGCCGCGTGGGCGGGCCTAGCGG
  CGAGAGGACAGAGTTGTGGGACGGCTGAGACCAACAAACACCTGATGCAACGAGCTGAGC
  CCGAAGTAAAAAACAGGAGGGACTATATAAAATAAGAATACTGTGCCAAACCACCGAAAT
  TCCACCACTGACCCCCGACCATCCCCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002731
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002731.2, NP_002722.1
• RefSeq Size: 4529 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5567
• Cytogenetics: 1p31.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (2) differs in the 5' exon structure, compared to variant 1. The encoded isoform (2, also known as Cbeta1) has a distinct N-terminus and is shorter than isoform 1.