NDUFAF1 (NM_016013) Human Untagged Clone

CAT#: SC108715

NDUFAF1 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 (NDUFAF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFAF1
• Synonyms: CGI-65; CGI65; CIA30; MC1DN11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108715 sequence for NM_016013 edited (data generated by NextGen Sequencing)
  ATGGCTTTGGTTCACAAATTGCTGCGTGGTACTTATTTTCTCAGAAAATTCTCTAAGCCA
  ACTTCTGCCTTGTATCCATTTTTGGGTATTCGCTTTGCAGAGTATTCCAGTAGTCTTCAG
  AAACCAGTGGCTTCTCCTGGCAAAGCCTCCTCACAGAGGAAGACTGAAGGGGATTTGCAA
  GGAGATCACCAGAAAGAAGTTGCTTTGGATATAACTTCTTCTGAGGAGAAGCCTGATGTT
  AGTTTCGATAAAGCAATTAGAGATGAAGCAATATACCATTTTAGGCTTTTGAAGGATGAA
  ATTGTGGATCATTGGAGAGGACCGGAAGGCCACCCTCTGCATGAGGTCTTGCTGGAACAA
  GCCAAGGTTGTCTGGCAATTCCGGGGGAAAGAAGATTTGGATAAGTGGACAGTGACTTCT
  GATAAGACGATTGGAGGCAGAAGTGAAGTGTTTTTGAAAATGGGCAAGAATAACCAAAGT
  GCACTGCTATATGGAACTCTGAGCTCTGAGGCGCCTCAGGACGGGGAGTCTACCCGAAGT
  GGGTACTGTGCAATGATATCCAGGATTCCAAGGGGTGCTTTTGAGAGGAAGATGTCTTAC
  GATTGGTCCCAGTTCAATACTCTGTATCTCCGTGTACGTGGGGATGGTCGGCCTTGGATG
  GTGAATATCAAGGAGGACACAGATTTCTTCCAGAGGACGAATCAGATGTATAGTTACTTC
  ATGTTCACCCGCGGGGGACCCTACTGGCAGGAGGTCAAGATTCCTTTTTCCAAATTTTTC
  TTCTCTAATCGAGGAAGAATCCGGGATGTTCAGCATGAGCTTCCGCTTGATAAGATCTCT
  TCTATAGGATTCACCTTGGCTGATAAAGTGGATGGTCCATTCTTCCTGGAGATAGATTTT
  ATTGGCGTGTTTACTGATCCAGCTCATACAGAAGAATTTGCCTATGAAAATTCTCCAGAG
  CTTAACCCAAGGCTTTTTAAATAA
  
  Clone variation with respect to NM_016013.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016013 unedited
  TCACCATTTTGTAATACGACTTACTATAGGGCGGCCCGCGAATTCGCACGAGGGCTCGGG
  GAGGTAAGCGGCGGCAGGCCGGCGGTTGGTGTGTCCCGGGTGTGGGGAGGCGACAGAGCC
  CTGGCACTTGAGGGTTGAGGGGGCCTCCCCAGCGCGGCGAACCGTCTAGCCTCCGGAGGC
  CAGGCCGTGAGTGCGGGAGGTATACGCCAAGGCGGAAGAATTTTGCCACTCACTACCTGT
  GTGACCTCGGGTAAATTAGCCTTGGAACGTCAGTTTCTTCGTCTCTATAATTGAAATAAT
  AATAGTACCTCTCTCAGGATTGTTGTGAGCCGTCAGTGAAACACTTAGAGCAGTTTCTGG
  CACATGGTAGAATTGGGCTATTTGCTGAAGCTTCTTGGTGGCCCTTGCTAGCCCAGGAAG
  AAACTTACATTTTGATTTTTTTGTACCATGGCTTTGGTTCACAAATTGCTGCGTGGTACT
  TATTTTCTCAGAAAATTCTCTAAGCCAACTTCTGCCTTGTATCCATTTTTGGGTATTCGC
  TTTGCAGAGTATTCCAGTAGTCTTCAGAAACCAGTGGCTTCTCCTGGCAAAGCCTCCTCA
  CAGAGGAAGACTGAAGGGGATTTGCAAGGAGATCACCAGAAAGAAGNTGCTTTGGATATA
  ACTTCTTCTGANGAGAAGCCTGATGCTAGTTTCGATAAAGCAATTAGAGATGAAGCCATA
  TACCATTTTANGCTTTTGAAAGATGAAATTGTGGATCATTGGAGAGGACGGNAAGCCACC
  CTCTGCATGANGTCCTGCTGGAACAGCCCAAGTTGTCTGGCCATTCCGGGGGAAGAAAGA
  TTGCATAATGGACAGTGACTTCTGATAAAACAATTGGAAGCACAAATGAAATGTTTTTGG
  AAATGGCCAGAATACCCAAGGGCCCTGCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016013 unedited
  NCCTGTCTTGAAGAAAATTTTATTTTGTCTATATCATTGTAGCAGCTAGTACCCTTAGAT
  TAGTAAAACAAAACTACCATATGATCTTTATTTAAAAAGCCTTGGGTTAAGCTCTGGAGA
  ATTTTCATAGGCAAATTCTTCTGTATGAGCTGGATCAGTAAACACGCCAATAAAATCTAT
  CTCCAGGAAGAATGGACCATCCACTTTATCAGCCAAGGTGAATCCTATAGAAGAGATCTT
  ATCAAGCGGAAGCTCATGCTGAACATCCCGGATTCTTCCTCGATTAGAGAAGAAAAATTT
  GGAAAAAGGAATCTTGACCTCCTGCCAGTAGGGTCCCCCGCGGGTGAACATGAAGTAACT
  ATACATCTGATTCGTCCTCTGGAAGAAATCTGTGTCCTCCTTGATATTCACCATCCAAGG
  CCGACCATCCCCACGTACACGGAGATACAGAGTATTGAACTGGGACCAATCGTAAGACAT
  CTTCCTCTCAAAAGCACCCCTTGGAATCCTGGATATCATTGCACAGTACCCACTTCGGGT
  AGACTCCCCGTCCTGAGGCGCCTCAGAGCTCAGAGTTCCATATAGCAGTGCACTTTGGTT
  ATTCTTGCCCATTTTCAAAAACACTTCACTTCTGCCTCCAATCGTCTTATCAGAAGTCAC
  TGTCCACTTATCCAAATCTTCTTTCCCCCGGAATTGCCAGACAACCTTGGCTTGTTCCAG
  CAAGACCTCATGCAGAGGGTGGCCTTNCGGTCCTCTCCATGATCCACAATTTCATCCTTC
  AAAGCCTAAAATGGTATATTGCTTCATCTCTAATTGGCTTATCGAAACTAACATCAGGCT
  TCTCCTCAGAAGAAGTATATCCAAAGCAACTTCTTTCTGGTGATCTNCTTGCAAATCCCC
  TTCAGTCTTTCTCTGTGAAGGAGCCTTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016013
• ORF Size: 984 bp
• Insert Size: 1640
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016013.2, NP_057097.2
• RefSeq Size: 1474
• RefSeq ORF: 984
• Locus ID: 51103
• Gene Summary: This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) represents the predominant transcript and is protein-coding.