NME2 (NM_002512) Human Untagged Clone

CAT#: SC108805

NME2 (untagged)-Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NME2
• Synonyms: NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002512, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAACCTGGAGCGCACCTTCATCGCCATCAAGCCGGACGGCGTGCAGCGCGGCCTGGTGGGCGAGA
  TCATCAAGCGCTTCGAGCAGAAGGGATTCCGCCTCGTGGCCATGAAGTTCCTCCGGGCCTCTGAAGAACA
  CCTGAAGCAGCACTACATTGACCTGAAAGACCGACCATTCTTCCCTGGGCTGGTGAAGTACATGAACTCA
  GGGCCGGTTGTGGCCATGGTCTGGGAGGGGCTGAACGTGGTGAAGACAGGCCGAGTGATGCTTGGGGAGA
  CCAATCCAGCAGATTCAAAGCCAGGCACCATTCGTGGGGACTTCTGCATTCAGGTTGGCAGGAACATCAT
  TCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCTATGGTTTAAGCCTGAAGAACTGGTT
  GACTACAAGTCTTGTGCTCATGACTGGGTCTATGAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002512 unedited
  GCACGAGGCCCGGGTGTGGTGGTCGCTCCAGCTCTCTGCTCTCCCAGCGCAGCGCCGCCG
  CCCGGCCCCTCCAGCTTCCCGGACCATGGCCAACCTGGAGCGCACCTTCATCGCCATCAA
  GCCGGACGGCGTGCAGCGCGGCCTGGTGGGCGAGATCATCAAGCGCTTCGAGCAGAAGGG
  ATTCCGCCTCGTGGCCATGAAGTTCCTCCGGGCCTCTGAAGAACACCTGAAGCAGCACTA
  CATTGACCTGAAAGACCGACCATTCTTCCCTGGGCTGGTGAAGTACATGAACTCAGGGCC
  GGTTGTGGCCATGGTCTGGGAGGGGCTGAACGTGGTGAAGACAGGCCGAGTGATGCTTGG
  GGAGACCAATCCAGCAGATTCAAAGCCAGGCACCATTCGTGGGGACTTCTGCATTCAGGT
  TGGCAGGAACATCATTCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCT
  ATGGTTTAAGCCTGAAGAACTGGTTGACTACAAGTCTTGTGCTCATGACTGGGTCTATGA
  ATAAGAGGTGGACACAACAGCAGTCTCCTTCAGCACGGCGTGGTGTGTCCCTGGACACAG
  CTCTTCATTCCATTGACTTAGAGGCAACAGGATTGATCATTCTTTTATAGAGCATATTTG
  CCAATAAAGCTTTTGGGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002512 unedited
  GCTTGCAAGCTTCTGGTTTATTGCTCTATAAAGNATGATCAATCCTGTTGCCTCTAAGTC
  AATGGAATGAAGAGCTGTGTCCAGGGACACACCACGCCGTGCTGAAGGAGACTGCTGTTG
  TGTCCACCTCTTATTCATAGACCCAGTCATGAGCACAAGACTTGTAGTCAACCAGTTCTT
  CAGGCTTAAACCATAGGCTGATTTCTTTTTCAGCACTTTTTACTGAATCACTGCCATGAA
  TGATGTTCCTGCCAACCTGAATGCAGAAGTCCCCACGAATGGTGCCTGGCTTTGAATCTG
  CTGGATTGGTCTCCCCAAGCATCACTCGGCCTGTCTTCACCACGTTCAGCCCCTCCCAGA
  CCATGGCCACAACCGGCCCTGAGTTCATGTACTTCACCAGCCCAGGGAAGAATGGTCGGT
  CTTTCAGGTCAATGTAGTGCTGCTTCAGGTGTTCTTCAGAGGCCCGGAGGAACTTCATGG
  CCACGAGGCGGAATCCCTTCTGCTCGAAGCGCTTGATGATCTCGCCCACCAGGCCGCGCT
  GCACGCCGTCCGGCTTGATGGCGATGAAGGTGCGCTCCAGGTTGGCCATGGTCCGGGAAG
  CTGGAGGGGCCGGGCGGCGGCGCTGCGCTGGGAGAGCAGAGAGCTGGAGCGACCACCACA
  CCCGGGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGAC
  GGTTCACTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGCCCATTT
  GCGTCAACGGGGCGGGGTTATTACGACATTTTGGGAAAGTCCCGTTGATTTTGGTGCCAA
  ACATACTCCCATGACGTCATGGGGTGGAGACTGGAAATCCCGGGAGTAAACCGTTNCACC
  CCATGTGTCTGCCAAACGCTCACATGTATAGCGAGACTATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002512
• Insert Size: 840 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002512.2, NP_002503.1
• RefSeq Size: 822 bp
• RefSeq ORF: 459 bp
• Locus ID: 4831
• Cytogenetics: 17q21.33
• Domains: NDK
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1-4 encode the same isoform.