ACCN2 (ASIC1) (NM_001095) Human Untagged Clone

CAT#: SC108885

ASIC1 (untagged)-Human amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASIC1
• Synonyms: ACCN2; ASIC; BNaC2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001095, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACTGAAGGCCGAGGAGGAGGAGGTGGGTGGCGTCCAGCCGGTGAGCATCCAGGCCTTCGCCAGCA
  GCTCCACACTGCACGGCCTGGCCCACATCTTCTCCTACGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGC
  CCTGTGCTTCCTGGGCTCGCTGGCTGTGCTGCTGTGTGTGTGCACGGAGCGTGTGCAGTACTACTTCCAC
  TACCACCATGTCACCAAGCTCGACGAGGTGGCTGCCTCTCAGCTTACCTTCCCTGCTGTCACGCTGTGCA
  ACCTCAACGAGTTCCGCTTTAGCCAAGTCTCCAAGAATGACCTGTATCATGCTGGGGAGCTGCTGGCCCT
  GCTCAACAACAGGTATGAGATACCAGACACACAGATGGCAGATGAAAAGCAGCTGGAGATACTGCAGGAC
  AAAGCCAACTTCCGCAGCTTCAAACCCAAACCCTTCAACATGCGTGAGTTCTACGACCGAGCTGGGCACG
  ACATTCGAGACATGCTGCTCTCCTGCCACTTCCGGGGGGAGGTCTGCAGCGCTGAAGACTTCAAGGTGGT
  CTTCACACGCTATGGAAAGTGCTACACGTTCAACTCGGGCCGAGATGGGCGGCCGCGGCTGAAGACCATG
  AAGGGTGGGACGGGCAATGGGCTGGAAATCATGCTGGACATCCAGCAGGACGAGTACCTGCCTGTGTGGG
  GGGAGACTGACGAGACGTCCTTCGAAGCAGGCATCAAAGTGCAGATCCATAGTCAGGATGAACCTCCTTT
  CATCGACCAGCTGGGCTTTGGCGTGGCCCCAGGCTTCCAGACCTTTGTGGCCTGCCAGGAGCAGCGGCTC
  ATCTACCTGCCCCCACCCTGGGGCACCTGCAAAGCTGTTACCATGGACTCGGATTTGGATTTCTTCGACT
  CCTACAGCATCACTGCCTGCCGCATCGACTGTGAGACGCGCTACCTGGTGGAGAACTGCAACTGCCGCAT
  GGTGCACATGCCAGGGGATGCCCCATACTGTACTCCAGAGCAGTACAAGGAGTGTGCAGATCCTGCTCTG
  GACTTCCTGGTGGAGAAGGACCAGGAGTACTGCGTGTGTGAAATGCCTTGCAACCTGACCCGCTATGGCA
  AAGAGCTGTCCATGGTCAAGATCCCCAGCAAAGCCTCAGCCAAGTACCTGGCCAAGAAGTTCAACAAATC
  TGAGCAATACATAGGGGAGAACATCCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATT
  GAACAGAAGAAGGCCTATGAGATTGCAGGGCTCCTGGGTGACATCGGGGGCCAGATGGGGCTGTTCATCG
  GGGCCAGCATCCTCACGGTGCTGGAGCTCTTTGACTACGCCTACGAGGTCATTAAGCACAAGCTGTGCCG
  ACGAGGAAAATGCCAGAAGGAGGCCAAAAGGAGCAGTGCGGACAAGGGCGTGGCCCTCAGCCTGGACGAC
  GTCAAAAGACACAACCCGTGCGAGAGCCTTCGGGGCCACCCTGCCGGGATGACATACGCTGCCAACATCC
  TACCTCACCATCCGGCCCGAGGCACGTTCGAGGACTTTACCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001095 unedited
  NGTTTCGCCATTTTGTAATACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGC
  AGACACTCGTAGGCGCGCACGCGCACCCTCTCCCCTCCCCCTCCCCTCCCGCCGCCTCCC
  GGCCGGACGATGGATCCCTGCAGATCCCAGGGCTGAGAGCACCGCGCACCGCGCCGAGCC
  CGGGCAGACCGAGCCGAGGCGAGCGAGCCAGCGAGCCAGCGCGCGCGGGCGGGCGGACAG
  ATCGGAGCCGAGCGGGGCCGGGCGGGGCGCTCCCTGCAGGGCTCTGCGCGGCGTGCCGCG
  GCGGCCGCGGGCTCCGGCCCCGGGCCATGAGCCCCTCCGCGACTCGGCGCTGAGCCCGCC
  ACCGGTCCAGCGCCCCAGGACCCGCCGCCGGCTGCCGGCTTGCCGAAGCCCCCTCAGGAT
  CCCCTCAACAAGGATGGAACTGAAGGCCGAGGAGGAGGAGGTGGGTGGCGTCCAGCCGGT
  GAGCATCCAGGCCTTCGCCAGCAGCTCCACACTGCACGGCCTGGCCCACATCTTCTCCTA
  CGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGCCCTGTGCTTCCTGGGCTCGCTGGCTGT
  GCTGCTGTGTGTGTGCACGGAGCGTGTGCAGTACTACTTTCACTACCACCATGTCACCCA
  AGCTCGACGAGGTGGCTGCCTCTCAGCTTACCTTCCCTGCTGTCACGCTGTGCAACCTCA
  ACGAGTTCCGCTTTAGCCAAGTCTTCCAAGATGACCTGTATTATGCTGGGGAGCTGCTGG
  CCCTGCTCAACACCGGTTTGAGATACCCGACCCACGNATGGCAGATTGAAAGCAGTTGGA
  GATACTGCAAGACAAAGGCCACTTTCGCAGCTTCAAACCCAAACCCTTAACATGCGTGGG
  TTCTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001095 unedited
  NATCTCTGNACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTGGGTGAAACCCACTTTATTAAACCAAAGGCATGCGTCACCCAAAACC
  CTGCCTGCTGTTCCCCTATCCAATGCACACTTTTTGGCCCCATTCCTGCTTCCCTCTGGG
  GAGGGATGAACTCTGAGGAAACTCTTAAGAACCAAAAAATTCCAAAGATCCTACAGCCTT
  ACTCTCCAACTCCAGGCCAATGCAGCTGCTCACAGGGCCTGTTCTGGGATTGAAAACCCA
  AACACAGCTAGGGAAAGAAGGCCTGAAAAGGGGTTGGGGCTCAAGCCTGGAAAGAATGTC
  TGGTGGAAGGAAAGGGGGGCAAGCCCTCCACCTCACTTGGCTCTCATTAAGATAGGGGGA
  GACAACAGCAGAGCTTTCAACTCCTCCTCTAAGCCTCTCTTTCCTCCCAAACCCACCTCA
  CCAAAAAATAAAAGTGGGGGGCTTGGGTATCAAGTGAGGAAAGGGGGCTGGGCACCCACA
  AAGAGGAAAGGCCACTGACTGAGGACCTCAGCTTCTGCCTGTCAGCATCCCACTGGCCCA
  ACTTCAGGAGTCGCCAATCCCCAGCTCCCTTCTGTCTCTGACACACTTGGCTTAATGGCC
  AGGGGAGACCTCCATCCCCTGGGGGGGGGGCTATCTCTACAGGAACCCCTTCCCTGGGGA
  TGCCACTGGCAACTGGGCAGAGGGCAATGGGATANGGAGGAGCAGGGGAGAAAATGTTTA
  AGCTGCAGGGAAGGCCCCAGGGGCCCCCGTTTGGGGAAAAAGGTAAAGCCCCAAAAAAGC
  AAACTTGTGATTCCAGCTTCCTTTGGCCCCCTGAACCAGGCAAGGGTACAAGCGCCGGGC
  CGCCCCTTGGCTTTAGAAGGGAGGGGGCCCCACACCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001095
• Insert Size: 4450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001095.2, NP_001086.2
• RefSeq Size: 3785 bp
• RefSeq ORF: 1587 bp
• Locus ID: 41
• Cytogenetics: 12q13.12
• Domains: ASC
• Protein Families: Druggable Genome, Ion Channels: Other
• Gene Summary: 'This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a.