p38 (MAPK14) (NM_139012) Human Untagged Clone

CAT#: SC109107

MAPK14 (untagged)-Human mitogen-activated protein kinase 14 (MAPK14), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK14
• Synonyms: CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_139012 edited
  ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTG
  CCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCT
  GCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAG
  TCCATCATTCATGCGAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACAT
  GAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAATTCAAT
  GATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGTGAAATGTCAG
  AAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT
  ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAA
  GACTGTGAGCTGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACA
  GGCTACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTAC
  AACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGAGCTGTTGACTGGAAGA
  ACATTGTTTCCTGGTACAGACCATATTGATCAGTTGAAGCTCATTTTAAGACTCGTTGGA
  ACCCCAGGGGCTGAGCTTTTGAAGAAAATCTCCTCAGAGTCTGCAAGAAACTATATTCAG
  TCTTTGACTCAGATGCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTG
  GCTGTCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCC
  CAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCC
  GATCCTTATGATCAGTCCTTTGAAAGCAGGGACCTCCTTATAGATGAGTGGAAAAGCCTG
  ACCTATGATGAAGTCATCAGCTTTGTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCC
  TGAGCACCTGGTTTCTGTTCTGTTGATCCCACTTCACTGTGAGGGGAAGGCCTTTTCACG
  GGAACTCTCCAAATATTATTCAAGTGCCTCTTGTTGCAGAGATTTCCTCCATGGTGGAAG
  GGGGTGTGCGTGCGTGTGCGTGCGTGTTAGTGTGTGTGCATGTGTGTGTCTGTCTTTGTG
  GGAGGGTAAGACAATATGAACAAACTATGATCACAGTGACTTTACAGGAGGTTGTGGATG
  CTCCAGGGCAGCCTCCACCTTGCTCTTCTTTCTGAGAGTTGGCTCAGGCAGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139012 unedited
  NGGTTCANATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCAGCT
  GGAACGGGAGTACTGCGACGCAGCCCGGAGTCGGCCTTGTAGGGGCGAAGGTGCAGGGAG
  ATCGCGGCGGGCGCAGTCTTGAGCGCCGGAGCGCGTCCCTGCCCTTAGCGGGGCTTGCCC
  CAGTCGCAGGGGCACATCCAGCCGCTGCGGCTGACAGCAGCCGCGCGCGCGGGAGTCTGC
  GGGGTCGCGGCAGCCGCACCTGCGCGGGCGACCAGCGCAAGGTCCCCGCCCGGCTGGGCG
  GGCAGCAAGGGCCGGGGAGAGGGTGCGGGTGCAGGCGGGGGCCCCACAGGGCCACCTTCT
  TGCCCGGCGGCTGCCGCTGGAAAATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGC
  TGAACAAGACAATCTGGGAGGTGCCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTG
  GCGCCTATGGCTCTGTGTGTGCTGCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGA
  AGAAGCTCTCCAGACCATTTCAGTCCATCATTCATGCGAAAAGAACCTACAGAGAACTGC
  GGTTACTTAAACATATGAAACATGAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTG
  CAAGGTCTCTGGAGGAATTCAATGATGTGTATCTGGTGACCCATCTCATGGGGGCAGATC
  TGAACAACATTGTGAAATGTCAGAAGCTTACAGATGACCATGTTCAGTTCCTTATCTACC
  ANATCTNCGAGGTCTAAAGTATATACATTCAGCTGACATAATTCACAGGNACCCCTAAAC
  CTAGTATCTANCTGTGAATGAAGACTGTGAGCTGAAGATTCTGGNATTTGGACTGGNCTC
  GCCACAGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139012 unedited
  TTGGCCGCGGCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTAAATTTGAAAAAAGGC
  TTTATTTTAACCAGTGGTATTGTCTGACATCCTATACGGCATAACTGATTACAGCCAAGT
  TCATGAATACAAATAAAATAGCAATTTCCCTCATTCTCTCTTTTGTTTTCTGTTCAGAGA
  AATCAGGAGATGGGAGCATTATGCTCAGAAACCGAAGAGCTCTTCCAAGAGCTCCAGCTT
  AGAGTCCAGGCTTCCAGAGCATGCAGCCTCCTAACACGTATGTGGTCACATGTGCAAAGA
  CCTTTATTACAAAATATTCAGAGCAGTATTTCTGTAGGAAATCACACGAGCATTTACAAC
  CAAACATGGCTGATAAAATTCATCTTGCTGTGGATGGTAAGGATTTGAACTGCAATAAAG
  GAGATGAGGCCTTTACTTTAAAGGTTCACTTTCCTTGAAAGGGGGAGAGTCCCGGGTGAG
  AGGGAATCGCCATCAGTCTGCCTCCTTCCTTCTTGCTCCAGTTGACTCAGGGGGTCCACC
  CTGTCCCTCTGGAGTACATGTATCACTGCACTGATTGAGAAATGGAAACACCTTGGCCCA
  AATACAAGATTCTGTCACACAGCCAACACTGGAAGTGACTTTATACTTTCTTATTACACA
  AAAGGCTTAAATATATGAGTCCTCATGTAGAACAAAATGCCTGAAATTCAGCAAGTTATG
  AAAACGACACTTCTCCACCCCTGAGGATATTTTATCTTTATAAAGTTGAAATACAAAATA
  TTAAATTATTTGCCCTTTCTCCCATCAAAGGAATACATTCTCAAAGGATCCTACAATTAC
  AAAATCTGAACCAATTTATTCTCCCAACCAAGAGTCATGTTAACCTGCAAGAGACCTTTT
  CATAGAGTGAAAAGACCCTGTTACTTTTATTTAAGAATCCTTTAGAAAGACACAAAGGAG
  TTCTGGCCTATGGCTAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139012
• Insert Size: 3990 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139012.1, NP_620581.1
• RefSeq Size: 3757 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1432
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains a different segment within the coding region when compared to variant 1. The translation frame remains the same, and the resulting isoform 2 has an internal segment different from that of isoform 1.