EYA1 (NM_172060) Human Untagged Clone

CAT#: SC109203

EYA1 (untagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA1
• Synonyms: BOP; BOR; BOS1; OFC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172060 edited
  TCGAAGGAACACATTAAACTGGTGGATGCAGCAGATGTAAGCGCTGTGCAAACATCTCAA
  GCCAGTTCAGATGTTGCTGTTTCCTCAAGTTGCAGTTAAAACAGAGCCAATGAGCAGCAG
  TGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGG
  GAGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACAGATTTACCCTTC
  CAACAGACCATACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGG
  GCAAACACAGTTTACCACAGGAATGCAACAAGCTACAGCCTATGCCACGTACCCACAGCC
  AGGACAGCCGTACGGCATTTCCTCATATGGCATCAAGACTGAAGGTGGATTGTCACAGTC
  TCAGTCACCTGGACAGACAGGATTTCTCAGCTATGGCACAAGCTTCAGTACCCCTCAACC
  TGGACAGGCACCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAACATCATCAGGAAT
  ATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGTTCACAGCAGGACTA
  TCCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCACCGTA
  TCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCAC
  TTACCAGCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGC
  AGAGTACAGCACAATCCACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATT
  GCGTCGAGGTTCAGATGGGAAATCACGTGGACGGGGCCGAAGAAACAATAATCCTTCACC
  TCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGAGACAATCATTGT
  TTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGGGATCCACCCACTTC
  AGTTTCCCTTGGACTGCGAATGGAAGAAATGATTTTCAACTTGGCAGACACACATTTATT
  TTTTAATGACTTAGAAGAATGTGACCAAGTCCATATAGATGATGTTTCTTCAGATGATAA
  CGGACAGGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAG
  TGCTAACTTATGTTTGGCAACTGGTGTACGGGGTGGTGTGGACTGGATGAGAAAGTTGGC
  CTTCCGCTACAGACGGGTAAAAGAGATCTACAACACCTACAAAAATAATGTTGGAGGTCT
  GCTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGAC
  CGACTCCTGGTTGACACTGGCCCTGAAAGCACTCTCGCTCATTCACTCCCGGACAAACTG
  TGTGAATATTTTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGCTGTA
  TGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAAGA
  AAGCTGTTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGG
  AGATGGTGTAGAAGAAGAACAAGGAGCAAAAAAAGCACGCGATGCCCTTCTGGAGGATCT
  CCAGCCACTCGGACCTCATGGCCCTGCACCACGCCTTGGAACTGGAGTACCTGTAACAGC
  GCTCGGCACTTTGACAGCGCACAGCTGCTCTGTGACCAGGGACAGATCCAGCAGGCCCCA
  GTCTCGCATCAGCGCCGGCCTCCAGAACTTAGCAATTCGAAGGAACACATTAAACTGGTG
  GATGCAGCAGATGTAAGCGCTGTGCAAACATCTCAAGCCAGTTCAGATGTTGCTGTTTCC
  TCAAGTTGCAGTTAAAACAGAGCCAATGAGCAGCAGTGAAACAGCTTCAACGACAGCCGA
  CGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGGAGCAGTAGTTTCAGCCCACGACC
  AACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCATACCCACATATTCT
  CCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAAT
  GCAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTC
  ATATGGCATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCTGGACAGACAGGATT
  TCTCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCACCATACAGCTACCA
  GATGCAAGGTAGCAGTTTTACAACATCATCAGGAATATATACAGGAAATAATTCACTCAC
  AAATTCCTCTGGATTTAATAGTTCACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCA
  GGGTCAGTACGCACAGTATTATAACAGCTCACCGTATCCAGCACATTATATGACCAGCAG
  CAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCAGCTTCAAGAACCGCCATC
  TGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGCACAATCCACAGCCC
  ATCAACACCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATC
  ACGTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAG
  AGTGTTCATCTGGGACTTGGATGAGACAATCATTGTTTTCCACTCCTTGCTTACTGGGTC
  CTACGCCAACAGATATGGGAGGGATCCACCCACTTCAGTTTCCCTTGGACTGCGAATGGA
  AGAAATGATTTTCAACTTGGCAGACACACATTTATTTTTTAATGACTTAGAAGAATGTGA
  CCAAGTCCATATAGATGATGTTTCTTCAGATGATAACGGACAGGACCTAAGCACATATAA
  CTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAGTGCTAACTTATGTTTGGCAACTGG
  TGTACGGGGTGGTGTGGACTGGATGAGAAAGTTGGCCTTCCGCTACAGACGGGTAAAAGA
  GATCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCAGCTAAGAGGGAAGC
  CTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGTTGACACTGGCCCT
  GAAAGCACTCTCGCTCATTCACTCCCGGACAAACTGTGTGAATATTTTAGTAACAACTAC
  TCAGCTCATCCCAGCATTGGCGAAAGTCCTGCTGTATGGGTTAGGAATTGTATTTCCAAT
  AGAAAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTGTTTTGAGAGAATAATTCA
  AAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGGAGATGGTGTAGAAGAAGAACAAGG
  AGCAAAAAAAGCACGCGATGCCCTTCTGGAGGATCTCCAGCCACTCGGACCTCATGGCCC
  TGCACCACGCCTTGGAACTGGAGTACCTGTAACAGCGCTCGGCACTTTGACAGCGCACAG
  CTGCTCTGTGACCAGGGACAGATCCAGCAGGCCCCAGTCTCGCATCAGCGCCGGCCTCCA
  GAACTTAGCAATTCGAAGGAACACATTAAACTGGTGGATGCAGCAGATGTAAGCGCTGTG
  CAAACATCTCAAGCCAGTTCAGATGTTGCTGTTTCCTCAAGTTGCAGTTAAAACAGAGCC
  AATGAGCAGCAGTGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGG
  TTCAGCAATTGGGAGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACA
  GATTTACCCTTCCAACAGACCATACCCACATATTCTCCCTACCCCTTCCTCACAAACTAT
  GGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCTACAGCCTATGCCAC
  GTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGCATCAAGACTGAAGGTGG
  ATTGTCACAGTCTCAGTCACCTGGACAGACAGGATTTCTCAGCTATGGCACAAGCTTCAG
  TACCCCTCAACCTGGACAGGCACCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAAC
  ATCATCAGGAATATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGTTC
  ACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAA
  CAGCTCACCGTATCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACGACACCATC
  CACCAATGCCACTTACCAGCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTAC
  AGATCCCACAGCAGAGTACAGCACAATCCACAGCCCATCAACACCCATTAAAGATTCAGA
  TTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATCACGTGGACGGGGCCGAAGAAACAA
  TAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGA
  GACAATCATTGTTTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGGGA
  TCCACCCACTTCAGTTTCCCTTGGACTGCGAATGGAAGAAATGATTTTCAACTTGGCAGA
  CACACATTTATTTTTTAATGACTTAGAAGAATGTGACCAAGTCCATATAGATGATGTTTC
  TTCAGATGATAACGGACAGGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGC
  TGCAGCAACCAGTGCTAACTTATGTTTGGCAACTGGTGTACGGGGTGGTGTGGACTGGAT
  GAGAAAGTTGGCCTTCCGCTACAGACGGGTAAAAGAGATCTACAACACCTACAAAAATAA
  TGTTGGAGGTCTGCTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAAT
  TGAAGCCCTGACCGACTCCTGGTTGACACTGGCCCTGAAAGCACTCTCGCTCATTCACTC
  CCGGACAAACTGTGTGAATATTTTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAA
  AGTCCTGCTGTATGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAA
  AATAGGAAAAGAAAGCTGTTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTA
  TGTTGTTATAGGAGATGGTGTAGAAGAAGAACAAGGAGCAAAAAAAGCACGCGATGCCCT
  TCTGGAGGATCTCCAGCCACTCGGACCTCATGGCCCTGCACCACGCCTTGGAACTGGAGT
  ACCTGTAACAGCGCTCGGCACTTTGACAGCGCACAGCTGCTCTGTGACCAGGGACAGATC
  CAGCAGGCCCCAGTCTCGCATCAGCGCCGGCCTCCAGAACTTAGCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172060
• Insert Size: 3330 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172060.1, NP_742057.1
• RefSeq Size: 3734 bp
• RefSeq ORF: 1680 bp
• Locus ID: 2138
• Cytogenetics: 8q13.3
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (EYA1A) differs in the 5' UTR and lacks an exon in the 5' region, which results in translation initiation from an alternate in-frame upstream start codon compared to variant EYA1C. The resulting shorter isoform (2) has a distinct N-terminus compared to isoform 1.