Integrin beta 4 binding protein (EIF6) (NM_002212) Human Untagged Clone

CAT#: SC109359

EIF6 (untagged)-Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF6
• Synonyms: b(2)gcn; CAB; eIF-6; EIF3A; ITGB4BP; p27(BBP); p27BBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109359 sequence for NM_002212 edited (data generated by NextGen Sequencing)
  ATGGCGGTCCGAGCTTCGTTCGAGAACAACTGTGAGATCGGCTGCTTTGCCAAGCTCACC
  AACACCTACTGTCTGGTAGCGATCGGAGGCTCAGAGAACTTCTACAGTGTGTTCGAGGGC
  GAGCTCTCCGATACCATCCCCGTGGTGCACGCGTCTATCGCCGGCTGCCGCATCATCGGG
  CGCATGTGTGTGGGGAACAGGCACGGTCTCCTGGTACCCAACAATACCACCGACCAGGAG
  CTGCAACACATTCGCAACAGCCTCCCAGACACAGTGCAGATTAGGCGGGTGGAGGAGCGG
  CTCTCAGCCTTGGGCAATGTCACCACCTGCAATGACTACGTGGCCTTGGTCCACCCAGAC
  TTGGACAGGGAGACAGAAGAAATTCTGGCAGATGTGCTCAAGGTGGAAGTCTTCAGACAG
  ACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGTGTCTTCAGCAATCAGGGAGGGCTG
  GTGCATCCCAAGACTTCAATTGAAGACCAGGATGAGCTGTCCTCTCTTCTTCAAGTCCCC
  CTTGTGGCGGGGACTGTGAACCGAGGCAGTGAGGTGATTGCTGCTGGGATGGTGGTGAAT
  GACTGGTGTGCCTTCTGTGGCCTGGACACAACCAGCACAGAGCTGTCAGTGGTGGAGAGT
  GTCTTCAAGCTGAATGAAGCCCAGCCTAGCACCATTGCCACCAGCATGCGGGATTCCCTC
  ATTGACAGCCTCACCTGA
  
  Clone variation with respect to NM_002212.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002212 unedited
  ACTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCGCGAGGGAGCTTGTTA
  CTGGTTACTTGGCCTCATGGCGGTCCGAGCTTCGTTCGAGAACAACTGTGAGATCGGCTG
  CTTTGCCAAGCTCACCAACACCTACTGTCTGGTAGCGATCGGAGGCTCAGAGAACTTCTA
  CAGTGTGTTCGAGGGCGAGCTCTCCGATACCATCCCCGTGGTGCACGCGTCTATCGCCGG
  CTGCCGCATCATCGGGCGCATGTGTGTGGGGAACAGGCACGGTCTCCTGGTACCCAACAA
  TACCACCGACCAGGAGCTGCAACACATTCGCAACAGCCTCCCAGACACAGTGCAGATTAG
  GCGGGTGGAGGAGCGGCTCTCAGCCTTGGGCAATGTCACCACCTGCAATGACTACGTGGC
  CTTGGTCCACCCAGACTTGGACAGGGAGACAGAAGAAATTCTGGCAGATGTGCTCAAGGT
  GGAAGTCTTCAGACAGACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGTGTCTTCAG
  CAATCAGGGAGGGCTGGTGCATCCCAAGACTTCAATTGAAGACCAGGATGAGCTGTCCTC
  TCTTCTTCAAGTCCCCCTTGTGGCGGGGACTGTGAACCGAGGCAGTGAGGTGATTGCTGC
  TGGGATGGTGGTGAATGACTGGTGTGCCTTCTGTGGCCTGGACACACCCAGCACAGAGCT
  GTCAGTGGTGGAGAGTGTCTTCAAGCTGAATGAAGCCCAACCTAGCACCATTGCCACCAG
  CATGCGGGATTCCCTCATTGACAGCCTTCCCTGAGTCACCTTTCCAAGTTGTTCCATGGG
  CCTCCTGGCTTCTGGACTGTGGCCAACCTTTCTCCACATTCCGCCCATCTGTACCGNATG
  CTGTCAGGAAGGTGGCAGAAAGCCACCTGGACTGAGGGGCCTGGCACCCACCCTTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002212 unedited
  NCCCTTAGCTCTGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGC
  CCGGNAGGAACTGCACTTTAATGGGGTGGCACAGGACAGCAGAACCCTCAGCCAGCAGCC
  ACAGGGCCTGCCAGCCAGCACAACAGAGCAGGTTTTTGCAGTAATGATAGATCCAGGCGA
  TAAGCACAGGTGGAAAAGGGTTGGGTGCCCAGCCCCTCAGTCCCAGTGAGCTCTCTGCCA
  CCTCCCTGCCAGCATCCGGTACAGATTGGGCGGAATGTGGAGAAGGTTGGCCACAGTCCA
  GAGCCAGGAGCCCATGGAACAACTTGGAAGGTGACTCAGGTGAGGCTGTCAATGAGGGAA
  TCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAGCTTGAAGACACTCTCC
  ACCACTGACAGCTCTGTGCTGGTTGTGTCCAGGCCACAGAAGGCACACCAGTCATTCACC
  ACCATCCCAGCAGCAATCACCTCACTGCCTCGGTTCACAGTCCCCGCCACAAGGGGGACT
  TGAAGAAGAGAGGACAGCTCATCCTGGTCTTCAATTGAAGTCTTGGGATGCACCAGCCCT
  CCCTGATTGCTGAAGACACAGTAGCTTCCTACTAGCACCTGGTCGGCCACTGTCTGTCTG
  AAGACTTCCACCTTGAGCACATCTGCCAGAATTTCTTCTGTCTCCCTGTCCAAGTCTGGG
  TGGACCAAGGCCACGTAGTCATTGCAGGTGGTGACATTGCCCAAGGCTGAGAGCCGCTCC
  TCCACCGCCTATCTGCCTGTGTCTGGGAGGCCTGTGCGAATGTGNTGCAGCTCCTGGTCG
  GCGGTATTTGGTGGGTACCAGNAGACCGTGCCTGTTCCCCACACACATGCGCCCCGAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002212
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002212.2, NP_002203.1
• RefSeq Size: 1114 bp
• RefSeq ORF: 738 bp
• Locus ID: 3692
• Cytogenetics: 20q11.22
• Domains: eIF6
• Protein Families: Druggable Genome
• Gene Summary: 'Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 6 all encode isoform a.