PEX10 (NM_002617) Human Untagged Clone

CAT#: SC109564

PEX10 (untagged)-Human peroxisomal biogenesis factor 10 (PEX10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEX10
• Synonyms: NALD; PBD6A; PBD6B; RNF69
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109564 sequence for NM_002617 edited (data generated by NextGen Sequencing)
  ATGGCCCCGGCCGCCGCCAGCCCCCCGGAGGTGATCCGCGCGGCGCAGAAGGACGAGTAC
  TACCGCGGTGGGCTGCGGAGCGCGGCGGGCGGCGCCCTGCACAGCCTGGCGGGTGCGAGG
  AAGTGGCTGGAGTGGAGGAAGGAGGTTGAGCTGCTCTCAGATGTGGCCTACTTTGGCCTC
  ACCACACTTGCAGGCTACCAGACCCTGGGGGAGGAGTACGTCAGCATCATCCAGGTGGAC
  CCATCGCGGATACATGTGCCCTCCTCGCTGCGCCGTGGCGTGCTGGTGACGCTGCATGCC
  GTCCTGCCCTACCTGCTGGACAAGGCCCTGCTCCCCCTGGAGCAGGAGCTGCAGGCTGAC
  CCCGACAGTGGGCGACCCTTGCAGGGGAGCCTGGGGCCAGGTGGGCGTGGCTGCTCAGGG
  GCGCGGCGCTGGATGCGTCACCACACGGCCACCCTGACTGAGCAGCAGAGGAGGGCGCTG
  CTGCGGGCGGTCTTCGTCCTCAGACAGGGCCTCGCCTGCCTCCAGCGGCTACATGTTGCC
  TGGTTTTACATCCACGGTGTCTTCTACCACCTGGCCAAGAGGCTCACGGGGATCACGTAC
  CTCCGTGTCCGCAGCCTGCCCGGAGAGGACCTGAGGGCCCGTGTTAGCTACAGGCTGCTG
  GGGGTCATCTCACTGCTGCACCTGGTGCTGTCCATGGGGCTGCAGCTGTACGGTTTCAGG
  CAGCGGCAGCGAGCCAGGAAGGAGTGGAGGCTGCACCGCGGCCTGTCTCACCGCAGGGCC
  TCCTTGGAGGAGAGAGCCGTTTCCAGAAACCCCCTGTGCACCCTGTGCCTGGAGGAGCGC
  AGGCACCCAACAGCCACGCCCTGCGGCCACCTGTTCTGCTGGGAGTGCATCACCGCGTGG
  TGCAGCAGCAAGGCGGAGTGTCCCCTCTGCCGGGAGAAGTTCCCTCCCCAGAAGCTCATC
  TACCTTCGGCACTACCGCTGA
  
  Clone variation with respect to NM_002617.3
  291 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002617 unedited
  AATCTTTCCCCGCCCCTTGCCGCATTAGGGCGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGGGCCGGCGTGGCTGCTCGGGACCACCCGAACCCGCGGCCATGG
  CCCCGGCCGCCGCCAGCCCCCCGGAGGTGATCCGCGCGGCGCAGAAGGACGAGTACTACC
  GCGGTGGGCTGCGGAGCGCGGCGGGCGGCGCCCTGCACAGCCTGGCGGGTGCGAGGAAGT
  GGCTGGAGTGGAGGAAGGAGGTTGAGCTGCTCTCAGATGTGGCCTACTTTGGCCTCACCA
  CACTTGCAGGCTACCAGACCCTGGGGGAGGAGTACGTCAGCATCATCCAGGTGGACCCAT
  CGCGGATACATGTGCCCTCCTCGCTGCGCCGTGGCGTGCTGGTGACGCTGCATGCCGTCC
  TGCCCTACCTGCTGGACAAGGCCCTGCTCCCCCTGGAGCAGGAGCTGCAGGCTGACCCCG
  ACAGTGGGCGACCCTTGCAGGGGAGCCTGNGGCCAGGTGGGCGTGGCTGCTCAGGNNGCG
  CGCGCTGGATGCGTCACCACACGGCCACCCTGACTGAGCAGCANNNAGAGGCGCTGCTGC
  GGGCGGTCTTCGTCCTCAGACAGGGCCTCGCCTGCCCTCCAGCGCTACATGTTGCCTGGT
  TTTACATCCACNGTGTCTTCTACCCACCTGGCCAAGAGCTCACGGNGATCACGTACCTNC
  GTGTCCGCAGCCTGCCCGGAGAGGACCTGAGGNCCCCGTGTAGCTACNAGCTGCTGGGGG
  TCATCTNACTGCTGCACCTGNTGCTGTCCATGGGGCTGCANCTGTACCGTTTCAGGCAGN
  CGCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002617
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002617.3, NP_002608.1
• RefSeq Size: 2034 bp
• RefSeq ORF: 981 bp
• Locus ID: 5192
• Cytogenetics: 1p36.32
• Domains: RING, Pex2_Pex12
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a protein involved in import of peroxisomal matrix proteins. This protein localizes to the peroxisomal membrane. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2).