MEK5 (MAP2K5) (NM_145160) Human Untagged Clone

CAT#: SC109631

MAP2K5 (untagged)-Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2K5
• Synonyms: HsT17454; MAPKK5; MEK5; PRKMK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_145160 edited
  ATGTGAGCCTCTTTAACCTGTAATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGA
  GAACCAGGTGCTGGTAATTCGCATCAAGATCCCAAATAGTGGGCGCGGTGGACTGGACAG
  TGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGTGATAGGCCAGGTTCTGC
  CTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATTACAGTGA
  GAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGC
  AAGTAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTG
  GGGAACGGAACATACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCA
  GCCCAGCAGTCTCAGATTCACTTCCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGA
  AAAAAATACTAGCCAATGGCCAGATGAATGAACAAGACATACGATATCGGGACACTCTTG
  GTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCCGAGTGGGAAAATATTAG
  CTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATGTCTGAAT
  TGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTG
  TAGAAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATA
  GGAAAATGCCAGAACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTT
  ATTTGTGGAGTTTAAAGATTTTACATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACA
  CAAGAGGACAGGTTAAGCTGTGTGATTTTGGAGTTAGCACTCAGCTGGTGAATTCTATAG
  CCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAGGATTTCAGGGGAGCAGT
  ATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGCTTGCTCTTGGGA
  GGTTTCCATATCCTCAGATTCAGAAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGC
  AGTGCATTGTTGATGAGGATTCGCCCGTCCTTCCAGTTGGAGAGTTCTCGGAGCCATTTG
  TACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAGGCCAGCACCTGAAGAAT
  TGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTGTCCATGT
  GGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCGTGAGGCTGCCGCA
  GGGCACTGAAAGCCCAGGACCAGTAACCAAGGAGAACAACCCACCCGTCGCCCTTCTCCG
  TATGCTGCCTGCGCCAGAAGAGCTTTGCTGGGCCCTGGCTTCCCTGCCCTCGCCTTCACC
  TCTGTCAGCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145160 unedited
  NCGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCGCCGCC
  GCCGCAGCCGCCGCCGGTCCGCGCGGCCTCGGGTGGCCGGAGCTCAGCCTGCGCGCGCCG
  CGCCCTGTGTCTCCGGGTGGGGCAGAAGACTCGCCCCTTGAACCTCCCGCGGGGACTCTC
  CGTGGTGTGGCGGCCCTGGGGCTCTTTCTTAATAGCCCCGGACTGAGTCCCCTCCAGTCG
  AGGACCCTCTCCTAGTCCACTGACGAGCGGTGGACACCTGCCGCTGTATCTCCCCCAAAC
  CGAGTCCTTGCCCTGCTGCCTCCTCATACCCACACGGCGGCAGAGACCTTCACCATAGCG
  TTCGCTCAACTCCAGAACCTTCCGACCTCCGCTAGTTCCTGCGGGCCTTTGCCCGCTTCC
  CGGTGCACCCTCCCCGGGAGACACCTCAGACCCCCGACAGCCTGGGCAGGCTCGGTGCCT
  GCGGGTGCGTTCCTGATCACCCCTCCCCTCTTCCCTCCCCCTCATCCTCCATTCCCTTGT
  TTTCACCCTCTGTCCTCTGCCCGTCACTCCCCTTGTCACCTCTTGGAGCCCCCTCCTAAC
  CAGCGGCCAGTGGGTTTCCCATACCCCAGGATGTGAGCCTCTTTAACCTGTAATGCTGTG
  GCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGAT
  CCCAAATAGTGGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGG
  ATGTGCTGGATGTGATGGGCCAGGTTCTGCCTGAAAGCACACTACAGCATTTGAATATGA
  AATAGAGATGGTGATCGAATACAGGGAAAGTGATGAGGGAATGAGGCCATGCTGTCTATT
  ATATTCCCAGTATGGACAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145160 unedited
  TTTTTTTTTTTTTTTGTCACAAAGCATTAATACTTTTATTCCCATCAGTGATGCTGACGG
  GCCTGACCCTTTATAGAGAAAAACATTAGGAAAACAGAAGTGGGCAGGGACCCTGGGAGC
  CTCCATTCTCAATGCCCCACCCTTTACCTCTCTGCCAGTGTCAGCGCCTTCAAACCAGAA
  GGTATGGGGATGGCCTGGTGGGGTGGGCCACACATGGGGCTCCCCAGGCAAGGCCACCTG
  CTGACAGAGGTGAAGGCGAGGGCAGGGAAGCCAGGGCCCAGCAAAGCTCTTCTGGCGCAG
  GCAGCATACGGAGAAGGGCGACGGGTGGGTTGTTCTCCTTGGTTACTGGTCCTGGGCTTT
  CAGTGCCCTGCGGCAGCCTCACGGGGGCCCCTGCTGGCTCCGCCTCTCCTCCAGCGCCCG
  GCACACCCACATGGACACCACGGCGGCATTTCCATCATTGAACTGCACGATGAACGGGTG
  GCCCATCAATTCTTCAGGTGCTGGCCTTTCTTTTGGCTGTTTTCGCATACACTGAGTGAT
  GAAATGTACAAATGGCTCCGAGAACTCTCCAACTGGAAGGACGGGCGAATCCTCATCAAC
  AATGCACTGCAGAAGCTGGAGAGGCATTAAAGATCCCTGGTTTTTCTGAATCTGAGGATA
  TGGAAACCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145160
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145160.1, NP_660143.1
• RefSeq Size: 2381 bp
• RefSeq ORF: 1347 bp
• Locus ID: 5607
• Cytogenetics: 15q23
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A).