PTGER3 (NM_198716) Human Untagged Clone

CAT#: SC109653

PTGER3 (untagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTGER3
• Synonyms: EP3; EP3-I; EP3-II; EP3-III; EP3-IV; EP3-VI; EP3e; PGE2-R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109653 sequence for NM_198716 edited (data generated by NextGen Sequencing)
  ATGAAGGAGACCCGGGGCTACGGAGGGGATGCCCCCTTCTGCACCCGCCTCAACCACTCC
  TACACAGGCATGTGGGCGCCCGAGCGTTCCGCCGAGGCGCGGGGCAACCTCACGCGCCCT
  CCAGGGTCTGGCGAGGATTGCGGATCGGTGTCCGTGGCCTTCCCGATCACCATGCTGCTC
  ACTGGTTTCGTGGGCAACGCACTGGCCATGCTGCTCGTGTCGCGCAGCTACCGGCGCCGG
  GAGAGCAAGCGCAAGAAGTCCTTCCTGCTGTGCATCGGCTGGCTGGCGCTCACCGACCTG
  GTCGGGCAGCTTCTCACCACCCCGGTCGTCATCGTCGTGTACCTGTCCAAGCAGCGTTGG
  GAGCACATCGACCCGTCGGGGCGGCTCTGCACCTTTTTCGGGCTGACCATGACTGTTTTC
  GGGCTCTCCTCGTTGTTCATCGCCAGCGCCATGGCCGTCGAGCGGGCGCTGGCCATCAGG
  GCGCCGCACTGGTATGCGAGCCACATGAAGACGCGTGCCACCCGCGCTGTGCTGCTCGGC
  GTGTGGCTGGCCGTGCTCGCCTTCGCCCTGCTGCCGGTGCTGGGCGTGGGCCAGTACACC
  GTCCAGTGGCCCGGGACGTGGTGCTTCATCAGCACCGGGCGAGGGGGCAACGGGACTAGC
  TCTTCGCATAACTGGGGCAACCTTTTCTTCGCCTCTGCCTTTGCCTTCCTGGGGCTCTTG
  GCGCTGACAGTCACCTTTTCCTGCAACCTGGCCACCATTAAGGCCCTGGTGTCCCGCTGC
  CGGGCCAAGGCCACGGCATCTCAGTCCAGTGCCCAGTGGGGCCGCATCACGACCGAGACG
  GCCATTCAGCTTATGGGGATCATGTGCGTGCTGTCGGTCTGCTGGTCTCCGCTCCTGATA
  ATGATGTTGAAAATGATCTTCAATCAGACATCAGTTGAGCACTGCAAGACACACACGGAG
  AAGCAGAAAGAATGCAACTTCTTCTTAATAGCTGTTCGCCTGGCTTCACTGAACCAGATC
  TTGGATCCTTGGGTTTACCTGCTGTTAAGAAAGATCCTTCTTCGAAAGTTTTGCCAGATG
  AGAAAAAGAAGACTCAGAGAGCAAGAGGAATTTTGGGGAAATTAA
  
  Clone variation with respect to NM_198716.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198716 unedited
  TTCAGGAATTTGTAATACGACTCACTATAGGGGCGCGCCGCGCAATTCCGCACCAGGAAG
  GCGTGGCTCCCTCCCGGGCCACTGAGCCCGGGCGGGGCCGCGGCCGCGGTCCCAGCAGCG
  GAGTAGGGCGGCGGCTGCGCCCCGCACCATGGGGGGCAGCCCAGCCCCAGCCGCGGTAAA
  CGCCGACCTCCGCCGCCGCCCGCGCCGCGTCTGCCCCCTCCCGCTGCGGCTCTCTGGACG
  CCATCCCGTGGTGACCTCGAAGCCAACATGAAGGAGACCCGGGGCTACGGAGGGGATGCC
  CCCTTCTGCACCCGCCTCAACCACTCCTACACAGGCATGTGGGCGCCCGAGCGTTCCGCC
  GAGGCGCGGGGCAACCTCACGCGCCCTCCAGGGTCTGGCGAGGATTGCGGATCGGTGTCC
  GTGGCCTTCCCGATCACCATGCTGCTCACTGGTTTCGTGGGCAACGCACTGGCCATGCTG
  CTCGTGTCGCGCAGCTACCGGCGCCGGGAGAGCAAGCGCAAGAAGTCCTTCCTGCTGTGC
  ATCGGCTGGCTGGCGCTCACCGACCTGGTGCGGCAGCTTCTCACCACCCCGGTCGTCATC
  GTCGTGTACCTGTCCAAGCAGCGTTGGGAGCACATCGACCCGTCGGGGCGGCTCTGCACC
  TTTTTTCGGGCTGACCATGACTGTTTTTCGGGCTCTCCTCCGTGTTCATCGCCAGCGCCA
  TGGGCCGTCAGCGGGCGCTGGCCATCAGGGCGCCCCACTGGTTTGCGAGCCCATGAAGAC
  GCCTGCCACCCGCGCTGTGCTGCTCGGCGTGGTGGTGGGCCTGCTCCCCTTCCCCCTGGT
  GCCGGTGCTGGGGGGTGGGCCAATACACCGGTCCAAGGGCCCCGGACGCTGGGGCTTATT
  TAACACACCGGGCGAGGGGGCACCGGGCCTAGCTTTTCGCTAAAATTGGGGAGCCTTTTT
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198716 unedited
  CTAAATTTACTGTGNACCGCGGCCCGCTTNCTANGATCGAGTTTTTTTTTTTTTTTTTTT
  AAATTCACAAAACAATAGGACATAAGTTTAATTGATGTATGTAGATATAGTAAAAGTACA
  AAAACATGTATGNGAAACAGGACATAACAGCTTCTGGATAACAGTTATATTTGGGGGTGG
  GCTTGGGGGAAGAAGTAAAAGAGAATAGTTTGGGAGGTGGGTGTTTCTGTGATTTTTTTT
  TCTTTTACAAAAAGAGAGTCATGGAGCTTCCAGTGATGTGATCCTGGCAGAAAGGCAGGT
  TTTAATTTCCCCAAAATTCCTCTTGCTCTCTGAGTCTTCTTTTTCTCATCTGGCAAAACT
  TTCGAAGAAGGATCTTTCTTAACAGCAGGTAAACCCAAGGATCCAAGATCTGGTTCAGTG
  AAGCCAGGCGAACAGCTATTAAGAAGAAGTTGCATTCTTTCTGCTTCTCCGTGTGTGTCT
  TGCAGTGCTCAACTGATGTCTGATTGAAGATCATTTTCAACATCATTATCAGGAGCGGAG
  ACCAGCAGACCGACAGCACGCACATGATCCCCATAAGCTGAATGGCCGTCTCGGTCGTGA
  TGCGGCCCCACTGGGCACTGGACTGAGATGCCGTGGCCTTGGCCCGGCAGCGGGACACCA
  GGGCCTTAATGGTGGCCAGGTTGCAGGAAAAGGTGACTGTCAGCGCCAAGAGCCCCAGGA
  AGGCAAAGGCAGAGGCGAAGAAAGGTTGCCCCAGTTATGCGAAGAGCTAGTCCCGTTGCC
  CCCTCGCCCGGTGCTGATGAAGCACCACGTCCCGGGCCACTGGACGGTGTACTGGCCCAC
  GCCCAGCACCGGCAGCAGGGCGAAGGCAAGCACGGCCAGCCACACGCCGAGCACACAGCG
  CGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198716
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198716.1, NP_942009.1
• RefSeq Size: 1844 bp
• RefSeq ORF: 1125 bp
• Locus ID: 5733
• Cytogenetics: 1p31.1
• Protein Families: Druggable Genome, GPCR, Transcription Factors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]'
  Transcript Variant: This variant (6) lacks two coding exons compared to variant 1. The resulting protein (isoform 6) has a distinct and shorter C-terminus, as compared to isoform 1. Other names for this transcript are EP3F and EP3d.