ST3GAL3 (NM_006279) Human Untagged Clone

CAT#: SC109759

ST3GAL3 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 3 (ST3GAL3), transcript variant 10

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST3GAL3
• Synonyms: EIEE15; MRT12; SIAT6; ST3GALII; ST3Gal III; ST3GalIII; ST3N
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006279, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGT
  ATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCAGTGGTTCTTTCCTTTGA
  CTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTG
  CCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCT
  TGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTG
  GGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTG
  TCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGG
  GCAATGGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACT
  GAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCACCTACCCC
  GAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGC
  AGGACTTTAAGTGGTTGAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATC
  TGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCC
  GCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCA
  GTGTGGCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATGAG
  CACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAAGAGTCCTGGACGCAC
  AATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTG
  GCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006279 unedited
  AGCATTTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACCAGGCCCCGCGTCG
  GGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAA
  TCATGTGAAGATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTT
  TCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGA
  GGACTCCAGTAAGTATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGT
  GGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGGTACAGTGACAGCTGATTG
  GAATTTGTGGTGGTTCATTACAATGAAAAAAGGAAGTGGGTCACTCACAACGGAAATGCC
  AGTAGCATGTTAGGCTCATCATGTACAACCGTATTCCTTGAAATTCCATTCTGGGGCTCA
  GTAGCCTGAAGGTGAGGCTACAGTAGGACACCTGGCTTCAGGAACCTTCAAGAAGCTCTC
  TCTTTTGTCCTGNAACAGGATGTGACTGCACAGCCTGGAGCTCCCAACTGCACGTCAGAA
  TGCACAGTATGATCGGTTGGGCCTTCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATT
  AGCCACCAAGTACGCAAACTTTTCAGAGGGAGCCTTGCAGCCTGGCTATGCTTCAGCCTT
  GATGACGGCCATCGTCCCCCGGTTCTCCAGCCCACCCCCATGTTCCTGGATGACTCCTTT
  CGAGTGGCCTACAATCCCGAGTTCCGGCCGCCTTTTGGCATCAAGGCCAGAAAATCTGAT
  CAAAGCCTCTTGGCATCACCCAAAAATACCCCCGACCCCTGCCTTGACAAGCTCCGTTCC
  GCCGCTGCATCATTTGGGCCAAGAGGCCGTCTTGCCAACAACTCTGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006279
• Insert Size: 2580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006279.2, NP_006270.1
• RefSeq Size: 2271 bp
• RefSeq ORF: 1128 bp
• Locus ID: 6487
• Cytogenetics: 1p34.1
• Domains: Glyco_transf_29
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with a form of autosomal recessive nonsymdromic cognitive disability as well as infantile epileptic encephalopathy. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]'
  Transcript Variant: This variant (10) has multiple differences in the coding region but maintains the reading frame compared to variant 1. The resulting isoform (j, also called B1) is shorter compared to isoform a.