SRC (NM_198291) Human Untagged Clone

CAT#: SC109795

SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRC
• Synonyms: ASV; c-SRC; p60-Src; SRC1; THC6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198291 edited
  CATCGAGGTTTTGAGAGGCTAACTCTCCCAAAAAGGACCATGGGTAGCAACAAGAGCAAG
  CCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACGTGCACGGCGCT
  GGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGGCCGACGGCCAC
  CGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGTTCGGAGGCTTC
  AACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCGGTGGAGTGACC
  ACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGTCCTTCAAGAAA
  GGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGGCCCACTCGCTC
  AGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCGACTCCATCCAG
  GCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGTTACTGCTCAAT
  GCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGAAAGGTGCCTAC
  TGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGCACTACAAGATC
  CGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCAACAGCCTGCAG
  CAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCCTCACCACCGTG
  TGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGGAGATCCCTCGG
  GAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGGTGTGGATGGGG
  ACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCACGATGTCTCCA
  GAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGAAGCTGGTGCAG
  TTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACATGAGCAAGGGG
  AGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGCCTCAGCTGGTG
  GACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGAACTACGTCCAC
  CGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCAAAGTGGCCGAC
  TTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAGGTGCCAAATTC
  CCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCATCAAGTCGGAC
  GTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGGTGCCCTACCCT
  GGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGATGCCCTGCCCG
  CCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGAAGGAGCCTGAG
  GAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCACGTCCACCGAG
  CCCCAGTACCAGCCCGGGGAGAACCTCTAGGCACAGGCGGGCCCAGACCGGCTTCTCGGC
  TTGGATCCTGGGCTGGGTGGCCCCTGTCTCGGGGCTTGCCCCACTCTGCCTGCCTGCTGT
  TGGTCCTCTCTCTGTGGGGCTGAATTGCCAGGGGCGAGGCCCTTCCTCTTTGGTGGCATG
  GAAGGGGCTTCTGGACCTAGGGTGGCCTGAGAGGGCGGTGGGTATGCGAGACCAGCACGG
  TGACTCTGTCCAGCTCCCGCTGTGGCCGCACGCCTCTCCCTGCACTCCCTCCTGGAGCTC
  TGTGGGTCTCTGGAAGAGGAACCAGGAGAAGGGCTGGGGCCGGGGCTGAGGGTGCCCTTT
  TCCAGCCTCAGCCTACTCCGCTCACTGAACTCCTTCCCCACTTCTGTGCCACCCCCGGTC
  TATGTCGAGAGCTGGCCAAAGAGCCTTTCCAAAGAGGAGCGATGGGCCCCTGGCCCCGCC
  TGCCTGCCACCCTGCCCCTTGCCATCCATTCTGGAAACACCTGTAGGCAGAGGCTGCCGA
  GACAGACCCTCTGCCGCTGCTTCCAGGCTGGGCAGCACAAGGCCTTGCCTGGCCTGATGA
  TGGTGGGTGGGTGGGATGAGTACCCCCTCAAACCCTGCCCTCCTTAGACCTGAGGGACCC
  TTCGAGATCATCACTTCCTTGCCCCCATTTCACCCATGGGGAGACAGTTGAGAGCGGGGA
  TGTGACATGCCCAAGGCCACGGAGCAGTTCAGAGTGGAGGCGGGCTTGGAACCCGGTGCT
  CCCTCTGTCATCCTCAGGAACCAACAATTCGTCGGAGGCATCATGGAAAGACTGGGACAG
  CCCAGGAAACAAGGGGTCTGAGGATGCATTCGAGATGGCAGATTCCCACTGCCGCTGCCC
  GCTCAGCCCAGCTGTTGGGAACAGCATGGAGGCAGATGTGGGGCTGAGCTGGGGAATCAG
  GGTAAAAGGTGCAGGTGTGGAGAGAGAGGCTTCAATCGGCTTGTGGGTGATGTTTGACCT
  TCAGAGCCAGCCGGCTATGAAAGGGAGCGAGCCCCTCGGCTCTGGAGGCAATCAAGCAGA
  CATAGAAGAGCCAAGAGTCCAGGAGGCCCTGGTCCTGGCCTCCTTCCCCGTACTTTGTCC
  CGTGGCATTTCAATTCCTGGCCCTGTTCTCCTCCCCAAGTCGGCACCCTTTAACTCATGA
  GGAGGGAAAAGAGTGCCTAAGCGGGGGTGAAAGAGGACGTGTTACCCACTGCCATGCACC
  AGGACTGGCTGTGTAACCTTGGGTGGCCCCTGCTGTCTCTCTGGGCTGCAGAGTCTGCCC
  CACATGTGGCCATGGCCTCTGCAACTGCTCAGCTCTGGTCCAGGCCCTGTGGCAGGACAC
  ACATGGTGAGCCTAGCCCTGGGACATCAGGAGACTGGGCTCTGGCTCTGTTCGGCCTTTG
  GGTGTGTGGTGGATTCTCCCTGGGCCTCAGTGTGCCCATCTGTAAAGGGGCAGCTGACAG
  TTTGTGGCATCTTGCCAAGGGTCCCTGTGTGTGTGTATGTGTGTGCATGTGTGCGTGTCT
  CCATGTGCGTCCATATTTAACATGTAAAAATGTCCCCCCCGCTCCGTCCCCCAAACATGT
  TGTACATTTCACCATGGCCCCCTCATCATAGCAATAACATTCCCACTGCCAGGGGTTCTT
  GAGCCAGCCAGGCCCTGCCAGTGGGGAAGGAGGCCAAGCAGTGCCTGCCTATGAAATTTC
  AACTTTTCCTTTCATACGTCTTTATTACCCAAGTCTTCTCCCGTCCATTCCAGTCAAATC
  TGGGCTCACTCACCCCAGCGAGCTCTCAAATCCCTCTCCAACTGCCTAAGGCCCTTTGTG
  TAAGGTGTCTTAATACTGTCCTTTTTTTTTTTTTAACAGTGTTTTGTAGATTTCAGATGA
  CTATGCAGAGGCCTGGGGGACCCCTGGCTCTGGGCCGGGCCTGGGGCTCCGAAATTCCAA
  GGCCCAGACTTGCGGGGGGTGGGGGGGTATCCAGAATTGGTTGTAAATACTTTGCATATT
  GTCTGATTAAACACAAACAGACCTCAGAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198291 unedited
  NGGGAATTCAGTATTTGTTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCATC
  GAGGTTTTGAGAGGCTAACTGCTCCCAAAAAGGACCATGGGTCAGCAACAAGAGCAAGCC
  CAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACGTGCACGGCGCTGG
  CGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGGCCGACGGCCACCG
  CGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGTTCGGAGGCTTCAA
  CTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCGGTGGAGTGACCAC
  CTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGTCCTTCAAGAAAGG
  CGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGGCCCACTCGCTCAG
  CACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCGACTCCATCCAGGC
  TGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGTTACTGCTCAATGC
  AGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGAAAGGTGCCTACTG
  CCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGCACTACAAGATCCG
  CAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCAACAGCCTGCAGCA
  GCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCCTCACCACCGTGTG
  CCCCACGTCCAAGCCGCAGAACTCAGGCCTGGCCAAGGATGCCTGGGAGATCCATCGGGA
  GTCGCTGCNGCTGGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198291 unedited
  GGCCATGGCATGGCACTTCCGGTCCAGGAAAGCACTGGGGCAGGGTCACAGGCATGCCAC
  CCGGGTTCTGTTGCAAGAAACAGACTATCACCGCGGCCGCAATCTATAGTCGAGTTTTTT
  TTTTTTTTTTTTTTTTTTCAGAGGTCTGTTTGTGTTTAATCACACAATATGCAAAGTATT
  TACAACCAATTCTGGATACCCCCCCACCCCCCGCAAGTTTTTTTTTTGGAATTTCAGAGC
  CCCAGGCCCGGCCCATAGCCAGGGGTCCCCCAGGCCTCTGCATAGTCATCTGAAATCTAC
  AAAACACTGTTAAAAAAAAAAAAAGGACAGTTTTAACACACCTTACACAAAGGGCCTTAA
  GCAGTTGGAGAGGGATTTGAGAGCTCGCTGGGGTGAGTGAGCCCATATTTGACTGGAATG
  GACGGGATAACACTTGGGTAATAAAGCCCTTTGAAAGGAAAATTTGATCTTTCTTAGGCA
  TCGCCTGCTTGGTCCCCTTCCCCTCTGCAACGTCCTGCCTAGGTCTTTTACCCTTTTCAG
  TGGGTATGCTTTCTCCCTCCCACAGCGCTCTTTTTTCTTTCCCTTTTTGTCCGCATTCCC
  TCAGTGTCTCCCCCCCTGTGTTTTTTTTTCTTCCGCCCCTAGGCGTTCGGGGTCCCGTTC
  GTTTTGTTTTGCTTCGTTGCCGTGTTGTGCGCGCGACCGCCGCCGGCGTGTTTTTTGTTG
  TGGTTTTCCACCGCGGCTGGTGCGGCGGTTGGATGTTTTAGACGCCGGGTGAGTGCGTGT
  GATTCGGTAGCCTCCTATCGAGATGGAGTGTTGAGTCCCCCATTTTCCTTTTCGTTTATT
  GCTCCCCTCTTTTCGCCCCTTTTTTTTGTTTCTCCGCCCTCCCTGTCCTGTCAGTCTGGT
  TGTCTCGTTAATTCATTTATATTAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_198291
• Insert Size: 3711 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198291.1, NP_938033.1
• RefSeq Size: 4044 bp
• RefSeq ORF: 1611 bp
• Locus ID: 6714
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway
• Gene Summary: 'This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.