TNXB (NM_032470) Human Untagged Clone

CAT#: SC109841

TNXB (untagged)-Human tenascin XB (TNXB), transcript variant XB-S

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNXB
• Synonyms: EDS3; EDSCLL; EDSCLL1; HXBL; TENX; TN-X; TNX; TNXB1; TNXB2; TNXBS; VUR8; XB; XBS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109841 sequence for NM_032470 edited (data generated by NextGen Sequencing)
  ATGCGCCTCTCGTGGAGCGTGGCCCAGGGCCCCTTTGATTCCTTCGTGGTCCAGTATGAG
  GACACGAACGGGCAGCCCCAGGCCTTGCTCGTGGACGGCGACCAGAGCAAGATCCTCATC
  TCAGGCCTGGAGCCCAGCACCCCCTACAGGTTCCTCCTCTATGGCCTCCATGAAGGGAAG
  CGCCTGGGGCCCCTCTCAGCTGAGGGCACCACAGGGCTGGCTCCTGCTGGTCAGACCTCA
  GAGGAGTCAAGGCCCCGCCTGTCCCAGCTGTCTGTGACTGACGTGACCACCAGTTCACTG
  AGGCTCAACTGGGAGGCCCCACCGGGGGCCTTCGACTCCTTCCTGCTCCGCTTTGGGGTT
  CCATCACCAAGCACACTGGAGCCGCATCCGCGTCCACTGCTGCAGCGCGAGCTGATGGTG
  CCGGGGACGCGGCACTCGGCCGTGCTCCGGGACCTGCGTTCCGGGACTCTGTACAGCCTG
  ACACTGTATGGGCTGCGAGGACCCCACAAGGCCGACAGCATCCAGGGAACCGCCCGCACC
  CTCAGCCCAGTTCTGGAGAGCCCCCGTGACCTCCAATTCAGTGAAATCAGGGAGACCTCA
  GCCAAGGTCAACTGGATGCCCCCACCATCCCGGGCGGACAGCTTCAAAGTCTCCTACCAG
  CTGGCGGACGGAGGGGAGCCTCAGAGTGTGCAGGTGGATGGCCAGGCCCGGACCCAGAAA
  CTCCAGGGGCTGATCCCAGGCGCTCGCTATGAGGTGACCGTGGTCTCGGTCCGAGGCTTT
  GAGGAGAGTGAGCCTCTCACAGGCTTCCTCACCACGGTTCCTGACGGTCCCACACAGTTG
  CGTGCACTGAACTTGACCGAGGGATTCGCCGTGCTGCACTGGAAGCCCCCCCAGAATCCT
  GTAGACACCTATGACATCCAGGTCACAGCCCCTGGGGCCCCGCCTCTGCAGGCGGAGACC
  CCAGGCAGCGCGGTGGACTACCCCCTGCATGACCTTGTCCTCCACACCAACTACACCGCC
  ACAGTGCGTGGCCTGCGGGGCCCCAACCTCACTTCCCCAGCCAGCATCACCTTCACCACA
  GGGCTAGAGGCCCCTCGGGACTTGGAGGCCAAGGAAGTGACCCCCCGCACCGCCCTGCTC
  ACTTGGACTGAGCCCCCAGTCCGGCCCGCAGGCTACCTGCTCAGCTTCCACACCCCTGGT
  GGACAGACCCAGGAGATCCTGCTCCCAGGAGGGATCACATCTCACCAGCTCCTTGGCCTC
  TTTCCCTCCACCTCCTACAATGCACGGCTCCAGGCCATGTGGGGCCAGAGCCTCCTGCCG
  CCCGTGTCCACCTCTTTCACCACGGGTGGGCTGCGGATCCCCTTCCCCAGGGACTGCGGG
  GAGGAGATGCAGAACGGAGCCGGTGCCTCCAGGACCAGCACCATCTTCCTCAACGGCAAC
  CGCGAGCGGCCCCTGATCGTGTTTTGCGACATGGAGACTGATGGGGGCGGCTGGCTGGTG
  TTCCAGCGCCGCATGGATGGACAGACAGACTTCTGGAGGGACTGGGAGGACTATGCCCAT
  GGTTTTGGGAACATCTCTGGAGAGTTCTGGCTGGGCAATGAGGCCCTGCACAGCCTGACA
  CAGGCAGGTGACTACTCCATGCGCGTGGACCTGCGGGCTGGGGACGAGGCTGTGTTCGCC
  CAGTACGACTCCTTCCACGTAGACTCGGCTGCGGAGTACTACCGCCTCCACTTGGAGGGC
  TACCACGGCACCGCAGGGGACTCCATGAGCTACCACAGCGGCAGTGTCTTCTCTGCCCGT
  GATCGGGACCCCAACAGCTTGCTCATCTCCTGCGCTGTCTCCTACCGAGGGGCCTGGTGG
  TACAGGAACTGCCACTACGCCAACCTCAACGGGCTCTACGGGAGCACAGTGGACCATCAG
  GGAGTGAGCTGGTACCACTGGAAGGGCTTCGAGTTCTCGGTGCCCTTCACGGAAATGAAG
  CTGAGACCAAGAAACTTTCGCTCCCCAGCGGGGGGAGGCTGA
  
  Clone variation with respect to NM_032470.3
  375 t=>a;903 g=>a;916 g=>a;1208 a=>c;1457 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032470 unedited
  NNCCCNNNCCCCNNNNNCCNNNNNNCCTGTCAGATTTGTATACGATCATATAGGCGGCCG
  CGNAATTCGCACCAGCTGGGNATGACAGCCCCANAAAAGGACACACCAGCCCCAGAGTTA
  GCCCCAGAGGCCCCTGAGCCTCCTGAAGAGCCCCGCCTAGGAGTGCTGACCGTGACCGAC
  ACAACCCCAGACTCCATGCGCCTCTCGTGGAGCGTGGCCCAGGGCCCCTTTGATTCCTTC
  GTGGTCCAGTATGAGGACACGAACGGGCAGCCCCAGGCCTTGCTCGTGGACGGCGACCAG
  AGCAAGATCCTCATCTCAGGCCTGGAGCCCAGCACCCCCTACAGGTTCCTCCTCTATGGC
  CTCCATGAAGGGAAGCGCCTGGGGCCCCTCTCAGCTGAGGGCACCACAGGGCTGGCTCCT
  GCTGGTCAGACCTCAGAGGAGTCAAGGCCCCGCCTGTCCCAGCTGTCTGTGACTGACGTG
  ACCACCAGTTCACTGAGGCTCAACTGGGAGGCCCCACCGGGGGCCTTCGACTCCTTCCTG
  CTCCGCTTTGGGGTTCCATCACCAAGCACACTGGAGCCGCATCCGCGTCCACTGCTGCAG
  CGCGAGCTGATGGTGCCGGGGACGCGGCACTCGGCCGTGCTCCGGGACCTGCGTTCCGGG
  ACTCTGTACAGCCTGACACTGTATGGGCTGCGAGGACCCCACANGGCCGACAGCATCCAG
  GGAACCGNCCGCACCCTCAGCCCAGTTCTGGGAGAGCCCNCGTGACCTCCATTNCAGTGA
  AATCAGGGAGACCTCAGCCAAGGTCAACTGGATGCCCCCACATCCCGGGCGACAGCTTNC
  AAGTCTCCTACCGCTGGCGNACGNAGGGGAGCCTCANATGTGCCAGTGGATGGCCANNGC
  CCGNACCAGAACTCCAGGGCCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032470 unedited
  GGGGGGGGNNNNNNNNNNNNTTTTNNNNNAANAAAATCTTGNACCGCGCCGCATACTANG
  ATCGGTTTTTTTTTTTTTTTTTTTACCTCAGTTTCTCCTTTATTGCTCCCGTACGAACCG
  CTCCCCTCCCCCCTGTAAACACAGTGCTGCGAGATCGCTGGCAGAGAAGGCTTCCTCCAG
  CGGCTGGGTGGTGAAGGACCCTGGCTCTTCTCTCGGGGCGACCCCTCAGTGCTCGGCAGT
  CATACTGGGGTGCGAGAGAGGTGGGCAGCAGCTCAGCCTCCCCCCGCTGGGGAGCGAAAG
  TTTCTTGGTCTCAGCTTCATTTCCGTGAAGGGCACCGAGAACTCGAAGCCCTTCCAGTGG
  TACCAGCTCACTCCCTGATGGTCCACTGTGCTCCCGTAGAGCCCGTTGAGGTTGGCGTAG
  TGGCAGTTCCTGTACCACCAGGCCCCTCGGTAGGAGACAGCGCAGGAGATGAGCAAGCTG
  TTGGGGTCCCGATCACGGGCAGAGAAGACACTGCCGCTGTGGTAGCTCATGGAGTCCCCT
  GCGGTGCCGTGGTAGCCCTCCAAGTGGAGGCGGTAGTACTCCGCAGCCGAGTCTACGTGG
  AAGGAGTCGTACTGGGCGAACACAGCCTCGTCCCCAGCCCGCAGGTCCACGCGCATGGAG
  TAGTCACCTGCCTGTGTCAGGCTGTGCAGGNCCTCATTGCCCAGCCAGACTCTCCAGAGA
  TGTTCCCAAAAACATGGNCATAGTCCTCCCAGTCCCTCCAGAAGTCTGTCTGTCCATCCA
  TTGCGCGCTGGGACACCAGCCAGCCCGCCCCATCAGTCTCCATGTCGCAAACACGATCAN
  GGGCCCGCTCGCGTTTGCGTTGAGGAAAATGTGCCTGTCCTGNAGCCACCGGTCCGTCTG
  CATCTCTNCCGCAGTCTGGGGAAGGGGATCGCACCCACCCGTGTGAAAGAGGTGGACCCG
  GCGNNNAGAGCTCTGGCCCCATGCCTGGAACTGCATTGGAGAGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032470
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032470.2, NP_115859.2
• RefSeq Size: 3129 bp
• RefSeq ORF: 2022 bp
• Locus ID: 7148
• Cytogenetics: 6p21.33-p21.32
• Domains: FBG, FN3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (XB-S) is transcribed from a cryptic internal promoter sequence and is substantially shorter than variant 3 at the 5' end. It encodes isoform 2, which is identical to the C-terminus of the full-length protein, isoform 3.