Von Hippel Lindau (VHL) (NM_000551) Human Untagged Clone

CAT#: SC109881

VHL (untagged)-Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VHL
• Synonyms: HRCA1; pVHL; RCA1; VHL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000551 edited
  ATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTC
  GAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCG
  GAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGGCGGCCGCGG
  CCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGT
  CCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACG
  CTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGA
  GATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTC
  AATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAG
  CGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATC
  GTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGG
  CTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000551 unedited
  GGCTCAGATTGTAAACGACTTACTGTGGGCGGCAAAGCCCTTCGCACGAGCCTCGTGCCT
  ATTTCGGCACGAGGCCGCGGCGTCCCGCCCGGGTGGTCTGGATCGCGGAGGGAATGCCCC
  GGAGGGCGGACAACTGGGACGAGGCCGAGGTAAGCGCGGAGGAGGCAGGCGTCGAAGAGT
  ACGGCCCTGCAACAAGACGGCGGGGAGGAGTTGTTTTTCTGACGAGTCCGGCCCGGAAGA
  GTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGATATGGAGGCCGGGCGGCCGCGGCCCGT
  GCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCG
  CGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCC
  GCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAAAGATGC
  AGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGT
  TGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATG
  CCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAG
  GTCGCTCTACGAAGATCTGGAAGACCACCCATATGTGCAGAAAGACCTGGAGCGGCTGAC
  ACAGGAGCGCATTGCACATCACCGGATGGGAGATTGAAGATTTCTGTTGAAACTTACACT
  GGTTCATCTCAACTTTTGATGGGACTGGTGAGTCTTGATCTAGATACAGGACTGGGTTCT
  TTCTTAAGTTTCAAAGGGTCTCATTCTCAGAGTTAAATAGGCCCCATTGCTTAAAAGGAA
  ATTAACGGCCTTCCCTAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000551 unedited
  CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGGCAGGGTCTTGCCCT
  GTCCCCCAGGCTGGAGTGCAGTGGCATACTCATGGCTCACTGCCGCCTCCAATTCCTAAA
  CTCCAATGATCTTCTCACCTCAGCCTGCCAAGTAGCTAGGATTACAGATGTGTGCCGCCA
  TGCTAATTTTTAAAAATTTTTTGTATAGATGGGTTCTCACTATGAGCCCAGGCTCATCTT
  GAAGTTCTGGGCTCAAGCAATCCTCTCACCTTGGCTTCCCAAAGTACTGGGATTACAGGC
  GGGAGCCACCATGCCAGCCGTCACTCTCATTTTTGAGCACAGGAATTGTGCCCAAAGGCT
  AGGATAAGGAATTAAAGATGCATGCCAAGAAATACCCACAAACATTAATTATAAAAGGGA
  GACTATACTAATGTTTGTGAAAAGCTTATACTAACATAGAGCAATATTTAAGTTATAAAG
  TTAAATGAAAAAGGGTATAAAACTGTATGAACAGTACAATCTCAACTATGTAAAAACCAA
  CCAAAATCTGCCCTAAAAAGGCTGGAAGGAAATAGCTCAAAATGTTAATGTGATTGTATT
  TAAGAAGTGTATCTAATAGGGATCTTTTTTCTTCCTTTTTAGCTCTCTGCCCTTTCCAAA
  TGTTTCTCTACTGGGGAGATTTTTTTAAAAAGCTCTTAGGGAAGGAGTACCAACCAAAGT
  GGAAAAAAAAATCTATTATTTACCAAAAACTGAGTCTGAAAAAACCATTGAATTTGAGGT
  CATGACTCCAAGTCTACCCTTAAAGAATTTGCGCATCTATAAACCTTGGAACCGTCCCGC
  TTCCTCATGAATCTCTGTTAGACCAAAGCTGAAGTGGAACGCCCCTGGCCTGCTGAAAAA
  AAGCTGATAGCCGGCCGGGGCCCCACCCGGTTCCCCCTTTCGGAGCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000551
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000551.2, NP_000542.1
• RefSeq Size: 2968 bp
• RefSeq ORF: 642 bp
• Locus ID: 7428
• Cytogenetics: 3p25.3
• Domains: VHL
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• Gene Summary: 'Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.