Von Hippel Lindau (VHL) (NM_000551) Human Untagged Clone

CAT#: SC109881

VHL (untagged)-Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1


  "NM_000551" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol VHL
Synonyms HRCA1; pVHL; RCA1; VHL1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000551 edited
ATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTC
GAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCG
GAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGGCGGCCGCGG
CCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGT
CCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACG
CTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGA
GATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTC
AATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAG
CGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATC
GTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGG
CTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATTGA
>OriGene 5' read for NM_000551 unedited
GGCTCAGATTGTAAACGACTTACTGTGGGCGGCAAAGCCCTTCGCACGAGCCTCGTGCCT
ATTTCGGCACGAGGCCGCGGCGTCCCGCCCGGGTGGTCTGGATCGCGGAGGGAATGCCCC
GGAGGGCGGACAACTGGGACGAGGCCGAGGTAAGCGCGGAGGAGGCAGGCGTCGAAGAGT
ACGGCCCTGCAACAAGACGGCGGGGAGGAGTTGTTTTTCTGACGAGTCCGGCCCGGAAGA
GTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGATATGGAGGCCGGGCGGCCGCGGCCCGT
GCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCG
CGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCC
GCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAAAGATGC
AGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGT
TGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATG
CCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAG
GTCGCTCTACGAAGATCTGGAAGACCACCCATATGTGCAGAAAGACCTGGAGCGGCTGAC
ACAGGAGCGCATTGCACATCACCGGATGGGAGATTGAAGATTTCTGTTGAAACTTACACT
GGTTCATCTCAACTTTTGATGGGACTGGTGAGTCTTGATCTAGATACAGGACTGGGTTCT
TTCTTAAGTTTCAAAGGGTCTCATTCTCAGAGTTAAATAGGCCCCATTGCTTAAAAGGAA
ATTAACGGCCTTCCCTAGG
>OriGene 3' read for NM_000551 unedited
CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGGCAGGGTCTTGCCCT
GTCCCCCAGGCTGGAGTGCAGTGGCATACTCATGGCTCACTGCCGCCTCCAATTCCTAAA
CTCCAATGATCTTCTCACCTCAGCCTGCCAAGTAGCTAGGATTACAGATGTGTGCCGCCA
TGCTAATTTTTAAAAATTTTTTGTATAGATGGGTTCTCACTATGAGCCCAGGCTCATCTT
GAAGTTCTGGGCTCAAGCAATCCTCTCACCTTGGCTTCCCAAAGTACTGGGATTACAGGC
GGGAGCCACCATGCCAGCCGTCACTCTCATTTTTGAGCACAGGAATTGTGCCCAAAGGCT
AGGATAAGGAATTAAAGATGCATGCCAAGAAATACCCACAAACATTAATTATAAAAGGGA
GACTATACTAATGTTTGTGAAAAGCTTATACTAACATAGAGCAATATTTAAGTTATAAAG
TTAAATGAAAAAGGGTATAAAACTGTATGAACAGTACAATCTCAACTATGTAAAAACCAA
CCAAAATCTGCCCTAAAAAGGCTGGAAGGAAATAGCTCAAAATGTTAATGTGATTGTATT
TAAGAAGTGTATCTAATAGGGATCTTTTTTCTTCCTTTTTAGCTCTCTGCCCTTTCCAAA
TGTTTCTCTACTGGGGAGATTTTTTTAAAAAGCTCTTAGGGAAGGAGTACCAACCAAAGT
GGAAAAAAAAATCTATTATTTACCAAAAACTGAGTCTGAAAAAACCATTGAATTTGAGGT
CATGACTCCAAGTCTACCCTTAAAGAATTTGCGCATCTATAAACCTTGGAACCGTCCCGC
TTCCTCATGAATCTCTGTTAGACCAAAGCTGAAGTGGAACGCCCCTGGCCTGCTGAAAAA
AAGCTGATAGCCGGCCGGGGCCCCACCCGGTTCCCCCTTTCGGAGCTGG
Restriction Sites NotI-NotI     
ACCN NM_000551
Insert Size 2620 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000551.2, NP_000542.1
RefSeq Size 2968 bp
RefSeq ORF 642 bp
Locus ID 7428
Cytogenetics 3p25.3
Domains VHL
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
Gene Summary 'Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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