MGAT4B (NM_054013) Human Untagged Clone

CAT#: SC110259

MGAT4B (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT4B
• Synonyms: GNT-IV; GNT-IVB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110259 sequence for NM_054013 edited (data generated by NextGen Sequencing)
  ATGTCACGTGTGGCAGGCACAAGAACAGATGTCAATGAGCTATTGCAGAGGTGGACACCC
  AGATGTGTGCGCTGGCACACTGGAGGGGCCCGTAGGGTGGCTCTAGACCGCCCCCTCGTG
  ACGGCTTGCCTACCACCTGCAGGCGACGTTGTGGACGTTTACCAGCGGGAGTTCCTGGCG
  CTGCGCGATCGGTTGCACGCAGCTGAGCAGGAGAGCCTCAAGCGCTCCAAGGAGCTCAAC
  CTGGTGCTGGACGAGATCAAGAGGGCCGTGTCAGAAAGGCAGGCGCTGCGAGACGGAGAC
  GGCAATCGCACCTGGGGCCGCCTAACAGAGGACCCCCGATTGAAGCCGTGGAACGGCTCA
  CACCGGCACGTGCTGCACCTGCCCACCGTCTTCCATCACCTGCCACACCTGCTGGCCAAG
  GAGAGCAGTCTGCAGCCCGCGGTGCGCGTGGGCCAGGGCCGCACCGGAGTGTCGGTGGTG
  ATGGGCATCCCGAGCGTGCGGCGCGAGGTGCACTCGTACCTGACTGACACTCTGCACTCG
  CTCATCTCCGAGCTGAGCCCGCAGGAGAAGGAGGACTCGGTCATCGTGGTGCTGATCGCC
  GAGACTGACTCACAGTACACTTCGGCAGTGACAGAGAACATCAAGGCCTTGTTCCCCACG
  GAGATCCATTCTGGGCTCCTGGAGGTCATCTCACCCTCCCCCCACTTCTACCCTGACTTC
  TCCCGCCTCCGAGAGTCCTTTGGGGACCCCAAGGAGAGAGTCAGGTGGAGGACCAAACAG
  AACCTCGATTACTGCTTCCTCATGATGTACGCGCAGTCCAAAGGCATCTACTACGTGCAG
  CTGGAGGATGACATCGTGGCCAAGCCCAACTACCTGAGCACCATGAAGAACTTTGCACTG
  CAGCAGCCTTCAGAGGACTGGATGATCCTGGAGTTCTCCCAGCTGGGCTTCATTGGTAAG
  ATGTTCAAGTCGCTGGACCTGAGCCTGATTGTAGAGTTCATTCTCATGTTCTACCGGGAC
  AAGCCCATCGACTGGCTCCTGGACCATATTCTGTGGGTGAAAGTCTGCAACCCCGAGAAG
  GATGCGAAGCACTGTGACCGGCAGAAAGCCAACCTGCGGATCCGCTTCAAACCGTCCCTC
  TTCCAGCACGTGGGCACTCACTCCTCGCTGGCTGGCAAGATCCAGAAACTGAAGGACAAA
  GACTTTGGAAAGCAGGCGCTGCGGAAGGAGCATGTGAACCCGCCAGCAGAGGTGAGCACG
  AGCCTGAAGACATACCAGCACTTCACCCTGGAGAAAGCCTACCTGCGCGAGGACTTCTTC
  TGGGCCTTCACCCCTGCCGCGGGGGACTTCATCCGCTTCCGCTTCTTCCAACCTCTAAGA
  CTGGAGCGGTTCTTCTTCCGCAGTGGGAACATCGAGCACCCGGAGGACAAGCTCTTCAAC
  ACGTCTGTGGAGGTGCTGCCCTTCGACAACCCTCAGTCAGACAAGGAGGCCCTGCAGGAG
  GGCCGCACCGCCACCCTCCGGTACCCTCGGAGCCCCGACGGCTACCTCCAGATCGGCTCC
  TTCTACAAGGGAGTGGCAGAGGGAGAGGTGGACCCAGCCTTCGGCCCTCTGGAAGCACTG
  CGCCTCTCGATCCAGACGGACTCCCCTGTGTGGGTGATTCTGAGCGAGATCTTCCTGAAA
  AAGGCCGACTAA
  
  Clone variation with respect to NM_054013.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_054013 unedited
  TTGTAATACGACTTACTATAGGGCGGCCCGCGAATTCGCACGAGGGCTAATTCCCCTGGT
  TTTCTCCCCATGAGCATTAGTGTCAGTGTAATTTTAGAGTGACCCCAGCTATGTCACGTG
  TGGCAGGCACAAGAACAGATGTCAATGAGCTATTGCAGAGGTGGACACCCAGATGTGTGC
  GCTGGCACACTGGAGGGGCCCGTAGGGTGGCTCTAGACCGCCCCCTCGTGACGGCTTGCC
  TACCACCTGCAGGCGACGTTGTGGACGTTTACCAGCGGGAGTTCCTGGCGCTGCGCGATC
  GGTTGCACGCAGCTGAGCAGGAGAGCCTCAAGCGCTCCAAGGAGCTCAACCTGGTGCTGG
  ACGAGATCAAGAGGGCCGTGTCAGAAAGGCAGGCGCTGCGAGACGGAGACGGCAATCGCA
  CCTGGGGCCGCCTAACAGAGGACCCCCGATTGAAGCCGTGGAACGGCTCACACCGGCACG
  TGCTGCACCTGCCCACCGTCTTCCATCACCTGCCACACCTGCTGGCCAAGGAGAGCAGTC
  TGCAGCCCGCGGTGCGCGTGGGCCAGGGCCGCACCGGAGTGTCGGTGGTGATGGGCATCC
  CGAGCGTGCGGCGCGAGGTGCACTCGTACCTGACTGACACTCTGCACTCGCTCATCTCCG
  AGCTGAGCCCGCAGGAGAAGGAGGGACTCGTCATCGTGGTGCTGATCGCCGAGACTGACT
  CACAGTACACTTCGGCAGTGACAGAAGACATCAAGGCCTTGNTCCCCACGGAGATCCATT
  CTGGGCTCCTGGNAGTCATCTCACCCTTCCCCCACTTCTACCCTGACTTCTCCCGNCTNC
  GAAAGTCCTTTGGGGACCCCAAGAGAGAGTCAGGTGGAGGACCAACAGACCTCGATACTG
  CTCCTCAGAGTCGCGCATCCAAAGCTCTACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_054013 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTCTTCATTAACCCTTTATTAC
  AAGTCACGCTCTTATAGAAGTATATGTGGACTTACGTGAAAAAATCAAATGTATCCAAGA
  ATAAAAAACACAGCACATAAAGTAGTATATGCATTCCAGTGTTCGCGCCAGAGACGGCGG
  GCGCCCAAGTAAAAGCTCTTCTAAAACGGCCTGACTGGGGCAGGCCGGGTGCGAACGGTT
  CCGGGCCTCAGGCACAGTGTGGGGGCCGCCTGCCTCCTCCGCGGCCCGGCGGGCGGGGGC
  AGCACCAGCTCCTAGGGCCTCCGGGCCAGCGGCGGACCCCAGGCCGGCCCAAGCCCGACG
  CCAGGCAGAACCCTTTGGGCGGGGCCGTATCTGGCCCTCCGGGGACGGCAGTGACGACAC
  CCCCAGAAATGTGGGCTTCAGGGCTGGCCACAGGGTACCCTCAGAAGCCCGCAGCTTAGT
  CGGCCTTTTTCAGGAAGATCTCGCTCAGAATCACCCACACAGGGGAGTCCGTCTGGATCG
  AGAGGCGCAGTGCTTCCAGAGGGCCGAAGGCTGGGTCCACCTCTCCCTCTCCACTCCCTT
  GTAGAAGGAGCCGATCTGGAGGTAGCCGTCGGGGCTCCGAGGGTCCCGGAGGTTGGGGGT
  GCGGCCCTCCTGCAGGGCCTCCTTGTCTGACTGAGGGTTTTTCAAAAGGCAGCACCCTCC
  ACAGACGTGTTGAAAGAGCTTGCCCTTCCGGGTGCTCGATGGTTCCCACTGCGAAAAAAG
  AAACCGTTCCACTTTTAAAGGTCTGACAGTACCCGGAAACCGGCTGAATTCCCTCCGCGT
  AGAGGGTGACGGCCCCCAAAAATCTTTTCCGCCTGAAGGTCTTCTTTCAGGCGGACACGC
  CGGTCTGTCTAATAGCCTTTCGTTACCTTGTTGGCGGCCAAATCGCTTCTCGCTAGGACT
  GTTTCCAAATTTATGTACTTAACCCGCGCCCCTCCGCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_054013
• ORF Size: 1692 bp
• Insert Size: 2620
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_054013.1, NP_463459.1
• RefSeq Size: 2891
• RefSeq ORF: 1692
• Locus ID: 11282
• Domains: Glyco_transf_55
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains a distinct 5' UTR and includes an additional in-frame portion of the 5' coding region, compared to variant 1. The encoded isoform (2) has a longer, distinct N-terminus compared to isoform 1.