OSBPL9 (NM_024586) Human Untagged Clone

CAT#: SC110752

OSBPL9 (untagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OSBPL9
• Synonyms: ORP-9; ORP9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024586, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGGT
  TCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAAAATGATGAGAGGCTCTCG
  CAGAGGATGTGTTAGACTCAGAGGAGCTGTGATTGGTATAGACGATGAGGACGACAGCACCTTCACAATA
  ACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGAGAGAAGTGGATCCATGCCT
  TAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAGGTTTGGATTCAGGATTTGTTCCTAGTGTCCA
  AGATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGATTGAACAATTAAAGCTTTTT
  GATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAGACAACAA
  ATAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAGACCAGAGTAATGCGGA
  GAAGCACGCAGATGGAATGATAAGTACTATTAATCCCGTAGATGCAATATATCAACCTAGTCCTTTGGAA
  CCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCTCCAGAACCTGTTCAGTTGTGTAAGTCAGAGC
  AGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTACCTTGGGACATCATCAGACTCCTACACCAAA
  TAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCTCACTTCTCCAAGCCACGTGAACTTGTCTCCA
  AATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATGAATTTTATGATGCTGATGAATTCCATCAAA
  GTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGATCTGCCTCAGTCCTGACACACAGCAGCTCGGG
  AAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAATTCTTCCTTGTCCAATGGAACAAGTGATGCT
  GACCTGTTTGATTCACATGATGACAGAGATGATGATGCGGAGGCAGGGTCTGTGGAGGAGCACAAGAGCG
  TTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGGATCTTACTAAGGTAGTTCTTCCAACGTTTAT
  TCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTTTTTTGCACATCCGGACCTGTTTGTGAGCATT
  AGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTTGTGAAATGGTACCTCTCAGCCTTTCATGCGG
  GAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCATTTTGGGCGAGATTTTTCAGTGTCATTGGAC
  ATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTCAGAAGGACCAGTTCCCTGGGTTTCCAAAAAC
  AGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCACCCATTTCAGCCTTTTATGCTGAGTGTTTTA
  ACAAGAAGATACAATTCAATGCTCATATCTGGACCAAATCAAAATTCCTTGGGATGTCAATTGGGGTGCA
  CAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGATGAACATTACATTCTCACATTCCCCAATGGC
  TATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTAGGAGGAGAATGCAATATTAATTGTTCCAAAA
  CAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCTTCTATGGGGGCAAGAAGCACAGAATTACTGC
  CGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTCAATTGAAGGGGAATGGAATGGTGTGATGTAT
  GCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGATACCAAGAAGTTGCCTATAATCAAGAAGAAAG
  TGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCAGCCTTTGGAAGGATGTCACTTTCAACTTAAA
  AATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAGGCTTGAAGAAAGACAAAGAGCAGAAGCCCGA
  GAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTATTTCATGAAGATGGAGAATGCTGGGTTTATG
  ATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024586 unedited
  GGTGTCANATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCAAGAT
  GGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTA
  CCGTTGGTTCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAA
  AATGATGAGAGGCTCTCGCAGAGGATGTGTTAGACTCAGACCCGTGATGCTGATGAGCGA
  GAGAAGTGGATCCATGCCTTAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAGGT
  TTGGATTCAGGATTTGTTCCTAGTGTCCAAGATTTTGATAAGAAACTTACAGAAGCTGAT
  GCTTACCTACAAATCTTGATTGAACAATTAAAGCTTTTTGATGACAAGCTTCAAAACTGC
  AAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAGACAACAAATAGCATGGTA
  GAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATTAATCCCGTA
  GATGCAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACT
  GTGTTACCTCCAGAACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCA
  GTTGGACCTGTGTTGGCTACCTTGGGACATCATCAGACTCCTACACCAAATAGTACAGGC
  AGTGGCCATTCACCACCGAGTAGCAGTCTCACTTCTCCAAGCCACGTGAACTTGTCTNCA
  AATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATGAATTNTATGATGCTGATGAA
  TTCCATCAAGTGCTCATCCCCAAGCGCTAATAGATCTTCTGGATCTGCTCAGTCTGACAC
  ACAGCACTCGGAAATGTCTAAACGCCAGAACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024586 unedited
  TGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTAAAAAAAAGGGTGGAAAAATTACTTTATGAAGCCTTAAGCACTAAAAATAATTA
  ATTAAACTGTAATCCAGGATTAAATACAATTTAATAATAGTTCAATTCCAAAATAAAAGT
  TATTGTAGGTAAAACCATGAAATTTCCTAACACTTGATTTTAATACATTGGGCTAATTTT
  CTAAAACAACTCAAAGGAACCCATTTTTACAGTAGGCAGAATATTTATGAAAAAAATCTG
  GCATCAGGTATATTTATATATATGGATGGGGGGGTATACGTATGGGGGGGTATATATATG
  GGGGGGGGGGGGGGTATCCCGAGATTATTTGAACTAAAAAACAAGTTGGGTATTTTAACA
  GCAGTACTAAAGCGCAGAGTTTCAAACTTGGATTTATAAATGCTTTCAACGTGGGGGGTT
  TGGAAAAGGAAAAAACATCATTTGATTTTCAAAACCTGAAGTTTTTCTCAGGACTGAAGT
  CAAAATCGTAACTGCCACAGAGGGAAAAGGGAAGCTTTTCCCCTTAATTGTTCATCTGCG
  TCAGAAAGTCCAGTATCCCTGAGATAGGAACCACTGTAATTAAAAGATTGGAATGAACAG
  GTTTCTCCCAAAGGAAGATTGTTTGTTGCTGAATTATGCCTACTGCCCTGATCATCAGGT
  ATAAACTTTGCATCTTCCAACCTAATGCTTGGCAGCACCAAGACGTTTCAGTAATGGTTC
  ATCATAAACCCAGCTTTTTCCATCCTTATGAAATAACCTTGTCTCCCATGAATTTCCTTT
  TCTCTTCTTTCTGGGGCTTTGCTTTTTTGGTTTTTTACAGCCTGGGCTTTGTTTAGNTGG
  TGCATCAATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024586
• ORF Size: 2211 bp
• Insert Size: 2940
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_024586.3, NP_078862.2
• RefSeq Size: 2919
• RefSeq ORF: 2211
• Locus ID: 114883
• Domains: Oxysterol_BP, PH
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (6) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and includes an additional in-frame exon in its central coding region, compared to variant 1. The encoded isoform (e) has a longer and distinct N-terminus, and contains an additional internal segment, compared to isoform a. Isoform e contains both a pleckstrin homology domain and a sterol-binding domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. This transcript contains a uAUG that would result in an 18 aa N-terminal extension, but it has a weak Kozak signal, is not supported by the vast majority of human and homologous transcripts, and dAUG use is supported by data in PMID:19413330, which selectively purifies acetylated protein N-termini and shows that Ala-2 is acetylated following cleavage of Met-1 as encoded by the dAUG.