POLR2J2 (NM_032959) Human Untagged Clone

CAT#: SC110811

POLR2J2 (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2J2
• Synonyms: HRPB11B; POLR2J3; RPB11b1; RPB11b2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110811 sequence for NM_032959 edited (data generated by NextGen Sequencing)
  ATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGATCACCATT
  AACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCATGAACAAAGAAGACCACACA
  CTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCAAGTGCTATTTGCTGGCTAC
  AAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGTGCAGACCACGCCGGACTAC
  AGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCATCAGCGAGCTGTCCCTGCTG
  GAGGAGCGCTTCCGGACGTGCCTGCTTCCCCTTCGCCTTCTGCCGTGA
  
  Clone variation with respect to NM_032959.5
  84 g=>t;90 c=>t;102 c=>g;285 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032959 unedited
  TTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGGACACTTGGGGTCTGGA
  CGCAACGGCGGCGGGAGCATGAACGCCCCTCCAGCCTTTCGAGTCGTTCTTGCTCTTCGA
  GGGCGAGAAGATCACCATTAACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCAT
  GAACAAAGAAGACCACACACTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCA
  AGTGCTATTTGCTGGCTACAAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGT
  GCAGACCACGCCGGACTACAGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCAT
  CAGCGAGCTGTCCCTGCTGGAGGAGCGCTTCCGGACGTGCCTGCTTCCCCTTCGCCTTCT
  GCCGTGATTGTCAGTTTCCTGAGGCCTCCCCAGCCACGCTTCCTGTACAGCCTGCAGAAC
  TTCTGGCATGGAAAAGCCCCTGTGCAACTGGTAAAGATATCAATAAGCACCAGGAGGTAT
  CTAAATCCACCAGGAGCCATAGGCATCACGNTTGACGTCCATTTACCAGTCTTCCCTGGC
  AAGATTCTTCTGAATTGTGCTGCCTTGCCAAAGAAGTTTGGAAGGGGCCTGGGCCCAATG
  GGCTTGGGCCTTGAATCCCAACATTTTGGGAAACAATTCAAGTGGGATGTTATAAGGGCA
  AAGGGGTTCAGAACATCCTGTCCAACATGGTGACATTCCATCTCTACTAAAATACATAAG
  TAACTGGNNTTGNNNGNNNGGGGGGCCCCGGTGATCCCCCCTCCTCCAAAAGGTGAGGGC
  AGATAATCGCTTGACCTGGAGGGAAGAGGGGGGGGGGGGACCNAAAAATATGGCCTTTTG
  CTCCCACGGGGGGAAAAAAAGGAAAAACTCCCTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032959
• ORF Size: 348 bp
• Insert Size: 2060
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_032959.3, NP_116581.3
• RefSeq Size: 1727
• RefSeq ORF: 348
• Locus ID: 246721
• Domains: RNA_pol_L
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]