Smoothelin (SMTN) (NM_134270) Human Untagged Clone

CAT#: SC111019

SMTN (untagged)-Human smoothelin (SMTN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMTN
• Synonyms: FLJ35168; FLJ35365; FLJ35620; FLJ38597
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_134270, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACGAGGCCTTAGCTGGGCTGGATGAGGGAGCCCTTCGGAAGCTGCTGGAGGTCACAGCAGATC
  TGGCAGAGCGGCGGCGCATCCGCTCAGCCATCCGGGAACTGCAGCGGCAGGAGCTGGAGCGCGAGGAGGA
  GGCCCTGGCATCCAAGCGTTTCCGTGCCGAGCGGCAGGACAACAAGGAGAACTGGCTGCACTCTCAGCAG
  CGGGAAGCTGAGCAGCGGGCTGCCCTGGCACGGCTGGCAGGGCAGCTGGAGTCCATGAACGATGTGGAGG
  AATTGACTGCACTGTTGCGAAGCGCTGGTGAGTATGAGGAGCGCAAGCTGATCCGAGCTGCCATCCGCCG
  TGTACGGGCTCAGGAGATTGAGGCTGCCACCTTGGCTGGGAGGTTGTACAGCGGGCGTCCCAACAGTGGC
  TCAAGAGAGGACAGCAAGGGGCTAGCGGCACACAGGCTGGAACAGTGTGAGGTGCCAGAGCGAGAGGAAC
  AGGAACAGCAGGCAGAGGTTTCAAAGCCAACCCCCACCCCTGAAGGCACCAGCCAGGATGTGACCACAGT
  GACACTCCTGCTGCGAGCCCCACCTGGGAGCACATCCAGCTCACCTGCCTCACCCAGCAGTTCACCCACC
  CCTGCCTCTCCTGAGCCTCCATTGGAGCCTGCCGAGGCCCAGTGCCTTACAGCTGAGGTTCCAGGCAGCC
  CAGAGCCACCCCCCAGCCCACCCAAGACCACCAGCCCTGAGCCTCAGGAGTCTCCAACGCTCCCCAGCAC
  TGAGGGCCAGGTGGTCAACAAGCTTCTGTCTGGCCCCAAAGAGACCCCTGCTGCCCAGAGCCCCACCAGA
  GGCCCCTCTGACACCAAGAGAGCAGACGTGGCTGGACCCCGACCCTGCCAACGCTCCCTGTCGGTGCTCA
  GCCCCCGCCAACCAGCCCAGAACCGAGAGTCCACCCCCCTTGCCAGCGGACCTTCCTCATTCCAGCGGGC
  TGGCTCTGTGCGGGATCGTGTCCACAAGTTCACATCTGATTCTCCTATGGCTGCTAGGCTCCAGGATGGC
  ACACCCCAGGCTGCCCTAAGTCCCCTGACCCCCGCAAGGCTCCTGGGCCCCTCCCTCACCAGCACCACCC
  CTGCCTCCTCCTCCAGCGGCTCCTCCTCTCGGGGCCCCAGTGATACCTCCTCCCGGTTCAGCAAGGAGCA
  ACGAGGAGTAGCCCAGCCCCTGGCCCAGCTTCGAAGCTGCCCCCAGGAGGAGGGCCCCAGGGGGCGGGGC
  TTGGCTGCTAGGCCCCTTGAAAACAGAGCAGGGGGGCCTGTGGCACGTTCAGAGGAGCCTGGTGCCCCGC
  TGCCCGTGGCCGTCGGCACTGCCGAGCCAGGGGGCAGTATGAAGACCACATTCACCATCGAGATCAAGGA
  CGGCCGTGGCCAGGCCTCCACAGGCCGGGTGCTGCTGCCCACAGGCAACCAGAGGGCAGAACTGACACTG
  GGGCTGCGGGCGCCCCCGACCCTACTCAGCACCAGTAGTGGGGGCAAGAGCACCATCACCCGTGTCAACA
  GCCCTGGGACCCTGGCTCGGCTGGGCAGTGTCACTCATGTCACCAGCTTCAGCCATGCCCCCCCCAGTAG
  CCGAGGAGGCTGCAGCATCAAGATGGAAGCAGAGCCAGCAGAGCCTCTCGCTGCAGCAGTGGAAGCGGCC
  AATGGGGCTGAGCAGACCCGAGTGAACAAAGCACCAGAAGGGCGGAGCCCTCTGAGCGCTGAGGAGCTGA
  TGACTATTGAGGATGAAGGAGTCTTGGACAAGATGCTGGATCAGAGCACGGACTTTGAAGAGCGGAAGCT
  CATCCGGGCTGCACTTCGTGAGCTCCGACAAAGGAAGAGAGACCAGCGGGACAAGGAGCGGGAACGGCGG
  CTGCAGGAGGCACGGGGCCGGCCAGGGGAGGGGCGCGGCAACACAGCCACTGAGACCACCACGAGGCACA
  GCCAGCGGGCAGCTGATGGCTCTGCTGTCAGCACTGTTACCAAGACTGAGCGGCTCGTCCACTCCAATGA
  TGGCACACGGACGGCCCGCACCACCACAGTGGAGTCGAGTTTCGTGAGGCGCTCGGAGAATGGCAGTGGC
  AGCACCATGATGCAAACCAAGACCTTCTCCTCTTCCTCCTCATCCAAGAAGATGGGCAGCATCTTCGACC
  GCGAGGACCAGGCCAGCCCACGGGCCGGCAGCCTGGCGGCGCTCGAGAAACGGCAGGCCGAGAAGAAGAA
  AGAGCTGATGAAGGCGCAGAGTCTGCCCAAGACCTCAGCCTCCCAGGCGCGCAAGGCCATGATTGAGAAG
  CTGGAGAAGGAGGGCGCGGCCGGCAGCCCTGGCGGACCCCGCGCAGCCGTGCAGCGATCCACCAGCTTCG
  GGGTCCCCAACGCCAACAGCATCAAGCAGATGCTGCTGGACTGGTGTCGAGCCAAGACTCGCGGCTACGA
  GCACGTCGACATCCAGAACTTCTCCTCCAGCTGGAGTGATGGGATGGCCTTCTGTGCCCTGGTGCACAAC
  TTCTTCCCTGAGGCCTTCGACTATGGGCAGCTTAGCCCTCAGAACCGACGCCAGAACTTCGAGGTGGCCT
  TCTCATCTGCGGAGACCCATGCGGACTGCCCGCAGCTCCTGGATACAGAGGACATGGTGCGGCTTCGAGA
  GCCTGACTGGAAGATGCTGGTGGACTGTGTGCCCCTGGTGGAGGTGGACGACATGATGATCATGGGCAAG
  AAGCCTGACCCCAAGTGTGTCTTCACCTATGTGCAGTCGCTCTACAACCACCTGCGACGCCACGAACTGC
  GCCTGCGCGGCAAGAATGTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_134270 unedited
  TATAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGGTTGCTGAGCGGGCCTGGGACAGCGGGTCGCGGCACCTCCGGC
  CTGCGCGTGTCTAATCCGTCTGTCGGGTCCCGAAAGAGCTAAGCCGAGCCTGCGCCGGAC
  GGGTGGGCTGGACTGAGAGAATTCTCTGAGCTGGTGACAGGTGCCACAGGCACTGGGGAT
  CTCACCAGAAAGGAACCGACGGAGCTAGGGGCCAGCGAGATGGCGGACGAGGCCTTAGCT
  GGGCTGGATGAGGGAGCCCTTCGGAAGCTGCTGGAGGTCACAGCAGATCTGGCAGAGCGG
  CGGCGCATCCGCTCAGCCATCCGGGAACTGCAGCGGCAGGAGCTGGAGCGCGAGGAGGAG
  GCCCTGGCATCCAAGCGTTTCCGTGCCGAGCGGCAGGACAACAAGGAGAACTGGCTGCAC
  TCTCAGCAGCGGGAAGCTAGNCAGCGGGGCTGCCCTGGCACGGCTGGCAGGGCAGCTGGA
  GTCCATGAACGATGTGGAGGAATTGACTGCACTGTTGCGAAGCGCTGGTGAGTATGAGGA
  GCGCAAGCTGATCCGAGCTGCCATCCGCCGTGTACGGGCTCAGGAGATTGAGGCTGCCAC
  CTTGGCTGGGGAGGTTGTACAGCGGGCGTCCCAACAGTGGCTCAAGAGAGGACCAGCAGG
  GGGCTAGCGGCACACAGGCTGGGACAGTGGGGAGGTGCCAGAGCGAGAGGGACAGGAACA
  GCAGCCAGAGGTTTTCAAGCCCACCCCCACCCCTGAAGGCACAGCCCAGATGTGACCCCA
  GTGACACTTCTGCTGGGAGCCCCACCTGGGAGCAATCCGGCTTAACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_134270 unedited
  GGGGGCGATATTAGCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTT
  TTTTCGTTGAAAACCAATAATTTATCAAAACGCTGCGTGTGTATGTGGGGGGGAGGGTGT
  CGCAACAGACAGGGCAGCGGTGGGCGGACGCACAGGCAGGAGACGGTGCCCGGAGAGTGG
  GGGCGGCAGCTTGCCACTGGCTGGCCATGCGGGCGGGCAGGCTAGACATTCTTGCCGCGC
  AGGCGCAGTTCGTGGCGTCGCAGGTGGTTGTAGAGCGACTGCACATAGGTGAAGACACAC
  TTGGGGTCAGGCTTCTTGCCCATGATCATCATGTCGTCCACCTCCACCAGGGGCACACAG
  TCCACCAGCATCCGTGGGGCCCCGAGCAGGGGTTAGGACTTTTTGGTTTTTACCAGCCCC
  TTCTGGACCAGACAGCGGTAGAATTCCTGGATGTACGTGTACACGCACTTCCAGTCAGGC
  TCTCGAAGCCGCACCATGTCCTCTGTATCCAGGAGCTGCGGGCAGTCCGCATGGGTCTCC
  GCAGATGAGAAGGCCACCTCGAAGTTCTGGCGTCCGTTCTGAGGGCTAAACTGCCCATAG
  TCGAAGGCCTCAGGGAAAAAATTGTGCACCACGGCACAGAAGGCCCTCCCCTCACTCCAA
  CTGGAGGAGAAATTTCTGGATGTCGACCGGCTCGTAACCGCGAGTCTTGTGTTGAAACCC
  ATTCAGCAACATCCTGCTTGAAGCTGTTTGGCCTTGGGGGACCCCCAAAACTGGGGGGAA
  CGTTTCACGGGCTGGCGCGGGGGTCCCCCCGGGTTTTTAGGACGCGCCCCTTCATTTTCC
  AATTTTTTAAATACAGGGCCTTGAGCGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_134270
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134270.1, NP_599032.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 2823 bp
• Locus ID: 6525
• Cytogenetics: 22q12.2
• Domains: CH
• Gene Summary: 'This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional in-frame exon in the 3' region, compared to variant 4. The resulting isoform (a, also referred to as B3) is shorter at the N-terminus, lacks a segment in the middle region and has an additional segment in the C-terminal region, compared to isoform d.