SURF5 (MED22) (NM_133640) Human Untagged Clone
CAT#: SC111029
MED22 (untagged)-Human mediator complex subunit 22 (MED22), transcript variant b
"NM_133640" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MED22 |
Synonyms | MED24; SRB6; surf-5; SURF5 |
Vector | PCMV6-Neo |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_133640, the custom clone sequence may differ by one or more nucleotides
ATGGCCCAGCAGAGAGCCCTGCCCCAGAGCAAGGAGACGCTGCTGCAGTCCTACAACAAGCGGCTGAAGG ACGACATTAAGTCCATCATGGACAACTTCACCGAGATCATCAAGACCGCCAAGATTGAGGACGAGACGCA GGTGTCACGGGCCACTCAGGGTGAACAGGACAATTACGAGATGCATGTGCGAGCCGCCAACATCGTCCGA GCCGGCGAGTCCCTGATGAAGCTGGTGTCCGACCTCAAGCAGTTCCTGATCCTCAATGACTTCCCCTCCG TGAACGAGGCCATTGACCAGCGCAACCAGCAGCTGCGCACACTGCAGGAGGAGTGCGACCGGAAGCTCAT CACGCTGCGAGACGAGATCTCCATTGACCTCTACGAGCTGGAGGAGGAGTATTACTCGTCCAGCTCAAGC CTTTGCGAAGCTAATGACCTGCCTCTGTGCGAAGCTTACGGGAGGCTGGACCTCGACACAGACTCTGCTG ATGGCCTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCCCAGTGCTGGCCCCCTACAGGTGGCAGCCCCTGC CCACTCCCATGCTGGTGGCCCTGGCCCCACTGAGCACGCCTGA >OriGene 5' read for NM_133640 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCAGAGAGCTCTTTGCAGTGT AGTGACACTGGGGTTTCTGCAGTCAGGGAGGAGGGAGGGTGGCCAGGCTGACAGCTTTTT GCAAGAGGAGGGGGACCAGCACCAGCTGGGAGGCATAGGCTAGGACAGGCCCACGTGGAG GCTGGGCAGGAAGGGCCTGCTGAGGTCACACAGCTGTTGGTGGTTGGGCCAGGGCGGCTT CCTCCTTTCAGAATGCTAGGGTGGCTCTCACCACTGGCCGCCTCTCCTTGCCAGGCCTGC CAACTCAGGGGACAGATGGAGCAGGAGTGGAGAAAGGGAAAGGCAGGTCTGGGGTGTGGT CGTGTTTTCTTAACTCTGCTTCTGTCTTGCTCTCCCCTCCCCTGGCTTTCCTCTCTGCCT GCTCCTGTCTCTCCCTGGGGTTTCTGGTGGTGGAAAAGCTCAAGCCTTTGCGAAGCTAAT GACCTGCCTCTGTGCGAAGCTTACGGGAGGCTGGACCTCGACACAGACTCTGCTGATGGC CTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCCCAGTGCTGGCCCCCTACAGGTGGCAGCC CCTGCCCACTCCCATGCTGGTGGCCCCTGCCCCACTGAGCACGCCTGAGCCTNCGGGGCC ACNGCTTCGNTCTCAGGAACAAACCCTGAGCAGCCCTTTGGATGCCCTCACAGNCCTGCT TCTCTCAGCCTAGGNTCCCATTTGGGGACTNCANGACCCCANNACCACTNAGACTTCCTT GGGAAGCCGTTAGCCCAGGGTGGGTCCCGNCACGACAGTAGGAAACAGTTGTTCCCCTAC CATTNNCGATAGCCATCATTNCGAGCATCATCTTGTTGCTGGCTTCTGGCCACCAGTGGA AAAGTTTCAGCTAGTCTGGCCGTTGGGACTANCATGGGCAGGAGGGCCTATT |
Restriction Sites | NotI-NotI |
ACCN | NM_133640 |
Insert Size | 1300 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133640.3, NP_598395.1 |
RefSeq Size | 1448 bp |
RefSeq ORF | 603 bp |
Locus ID | 6837 |
Cytogenetics | 9q34.2 |
Gene Summary | 'This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]' Transcript Variant: This variant (b) represents the shorter transcript and encodes the longer isoform (b). |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212017 | MED22 (Myc-DDK-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 420.00 |
|
RG212017 | MED22 (GFP-tagged) - Human mediator complex subunit 22 (MED22), transcript variant b |
USD 460.00 |
|
RC212017L3 | Lenti-ORF clone of MED22 (Myc-DDK-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 620.00 |
|
RC212017L4 | Lenti-ORF clone of MED22 (mGFP-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 620.00 |
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