SURF5 (MED22) (NM_133640) Human Untagged Clone

CAT#: SC111029

MED22 (untagged)-Human mediator complex subunit 22 (MED22), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MED22
• Synonyms: MED24; SRB6; surf-5; SURF5
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133640, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCAGCAGAGAGCCCTGCCCCAGAGCAAGGAGACGCTGCTGCAGTCCTACAACAAGCGGCTGAAGG
  ACGACATTAAGTCCATCATGGACAACTTCACCGAGATCATCAAGACCGCCAAGATTGAGGACGAGACGCA
  GGTGTCACGGGCCACTCAGGGTGAACAGGACAATTACGAGATGCATGTGCGAGCCGCCAACATCGTCCGA
  GCCGGCGAGTCCCTGATGAAGCTGGTGTCCGACCTCAAGCAGTTCCTGATCCTCAATGACTTCCCCTCCG
  TGAACGAGGCCATTGACCAGCGCAACCAGCAGCTGCGCACACTGCAGGAGGAGTGCGACCGGAAGCTCAT
  CACGCTGCGAGACGAGATCTCCATTGACCTCTACGAGCTGGAGGAGGAGTATTACTCGTCCAGCTCAAGC
  CTTTGCGAAGCTAATGACCTGCCTCTGTGCGAAGCTTACGGGAGGCTGGACCTCGACACAGACTCTGCTG
  ATGGCCTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCCCAGTGCTGGCCCCCTACAGGTGGCAGCCCCTGC
  CCACTCCCATGCTGGTGGCCCTGGCCCCACTGAGCACGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133640 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCAGAGAGCTCTTTGCAGTGT
  AGTGACACTGGGGTTTCTGCAGTCAGGGAGGAGGGAGGGTGGCCAGGCTGACAGCTTTTT
  GCAAGAGGAGGGGGACCAGCACCAGCTGGGAGGCATAGGCTAGGACAGGCCCACGTGGAG
  GCTGGGCAGGAAGGGCCTGCTGAGGTCACACAGCTGTTGGTGGTTGGGCCAGGGCGGCTT
  CCTCCTTTCAGAATGCTAGGGTGGCTCTCACCACTGGCCGCCTCTCCTTGCCAGGCCTGC
  CAACTCAGGGGACAGATGGAGCAGGAGTGGAGAAAGGGAAAGGCAGGTCTGGGGTGTGGT
  CGTGTTTTCTTAACTCTGCTTCTGTCTTGCTCTCCCCTCCCCTGGCTTTCCTCTCTGCCT
  GCTCCTGTCTCTCCCTGGGGTTTCTGGTGGTGGAAAAGCTCAAGCCTTTGCGAAGCTAAT
  GACCTGCCTCTGTGCGAAGCTTACGGGAGGCTGGACCTCGACACAGACTCTGCTGATGGC
  CTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCCCAGTGCTGGCCCCCTACAGGTGGCAGCC
  CCTGCCCACTCCCATGCTGGTGGCCCCTGCCCCACTGAGCACGCCTGAGCCTNCGGGGCC
  ACNGCTTCGNTCTCAGGAACAAACCCTGAGCAGCCCTTTGGATGCCCTCACAGNCCTGCT
  TCTCTCAGCCTAGGNTCCCATTTGGGGACTNCANGACCCCANNACCACTNAGACTTCCTT
  GGGAAGCCGTTAGCCCAGGGTGGGTCCCGNCACGACAGTAGGAAACAGTTGTTCCCCTAC
  CATTNNCGATAGCCATCATTNCGAGCATCATCTTGTTGCTGGCTTCTGGCCACCAGTGGA
  AAAGTTTCAGCTAGTCTGGCCGTTGGGACTANCATGGGCAGGAGGGCCTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133640
• Insert Size: 1300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133640.3, NP_598395.1
• RefSeq Size: 1448 bp
• RefSeq ORF: 603 bp
• Locus ID: 6837
• Cytogenetics: 9q34.2
• Gene Summary: 'This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (b) represents the shorter transcript and encodes the longer isoform (b).