ATP6V0E1 (NM_003945) Human Untagged Clone

CAT#: SC111093

ATP6V0E1 (untagged)-Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V0E1
• Synonyms: ATP6H; ATP6V0E; M9.2; Vma21; Vma21p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111093 sequence for NM_003945 edited (data generated by NextGen Sequencing)
  ATGGCGTATCACGGCCTCACTGTGCCTCTCATTGTGATGAGCGTGTTCTGGGGCTTCGTC
  GGCTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGGAGTTATCATTACCATG
  TTGGTGACCTGTTCAGTTTGCTGCTATCTCTTTTGGCTGATTGCAATTCTGGCCCAACTC
  AACCCTCTCTTTGGACCGCAATTGAAAAATGAAACCATCTGGTATCTGAAGTATCATTGG
  CCTTGA
  
  Clone variation with respect to NM_003945.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003945 unedited
  GCACGAGGGCCGACCATGGTCGTATCACGGCCTCACTGTGCCTCTCATTGTGATGAGCGT
  GTTCTGGGGCTTCGTCGGCTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGG
  AGTTATCATTACCATGTTGGTGACCTGTTCAGTTTGCTGCTATCTCTTTTGGCTGATTGC
  AATTCTGGCCCAACTCAACCCTCTCTTTGGACCGCAATTGAAAAATGAAACCATCTGGTA
  TCTGAAGTATCATTGGCCTTGAGGAAGAAGACATGCTCTACAGTGCTCAGTCTTTGAGGT
  CACGAGAAGAGAATGCCTTCTAGATGCAAAATCACCTCCAAACCAGACCACTTTTCTTGA
  CTTGCCTGTTTTGGCCATTAGCTGCCTTAAACGTTAACAGCACATTTGAATGCCTTATTC
  TACAATGCAGCGTGTTTTCCTTTGCCTTTTTTGCACTTTGGTGAATTACGTGCCTCCATA
  ACCTGAACTGTGCCGACTCCACAAAACGATTATGTACTCTTCTGAGATAGAAGATGCTGT
  TCTTCTGAGAGATACGTTACTCTCTCCTTGGAATCTGTGGATTTGAAGATGGCTCCTGCC
  TTCTCACGTGGGAATCAGTGAAGTGTTTAGAAACTGCTGCAAGACAAACAAGACTCCAGT
  GNGGTGGTCAGTAGGAGAGCACGTTCAGAGGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003945 unedited
  TTGAACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTCCATAAAAAGGAGGAAAATATTTATTCCAAAAGAATGGCAAAAAAAAAAAAT
  TCATCCTGAAAGTATAGTTGGGGGCGATTCTGTTGAAATGGCTTTTCCTTTTGAACGTGC
  TTTCCTACTGACCACCCCACTGGAGTCTTGTTTGTCTTGCAGCAGTTTCTAAACACTTCA
  CTGATTCCCACGTGAAAAGGCAGGAGCCATTTTCAAATCCACAGATTCCAAGGAAAGAGT
  AACGTATTTTTCAAAAAAACAGCATTTTTTATTTCAAAAAAGTACATAATCGTTTTGGGG
  AGTCGGCACAGTTCAGGTTATGGAGGCACGTAATTCACCAAAGTGCAAAAAAGGCAAAGG
  AAAACACCCTGCATTGTAAAATAAGGCATTCAAATGTGCTGTTAACGTTTAAGGCAGCTA
  ATGGCCAAAACAGGCAAGTCAAAAAAAGTGGTCTGGTTTGGAGGTGATTTTGCATCTAAA
  AGGCATTCTCTTCTCGTGACCTCAAAAACTGACCACTGTAAAGCATGTCTTCTTCCTCAA
  GGCCAATGATACTTCAAATACCAAATGGTTTCATTTTTCAATTGCGGTCCAAAGAGAGGG
  TTGAGTTGGGCCAAAATTGCAATCAGCCAAAAGAGATAGCAGCAAACTGAACAGGTCACC
  AACATGGTAATGATAACTCCCCGGTTAGGACCCTTAGGGATGAACCAGGCACCAAGAAGC
  CGACGAAGCCCCAGACACGCTCATCACAATGAGAGGCCAGTGAGGCCGTGATCGCATGGT
  CGCCTCGTGCCGATTCCGGCCGCCTATAGTGAGTCGTATACAAAATCTGACGTTCACTAA
  CGAGCTTGCTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003945
• ORF Size: 246 bp
• Insert Size: 880
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003945.3, NP_003936.1
• RefSeq Size: 894
• RefSeq ORF: 246
• Locus ID: 8992
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008]