MTHFD1L (NM_015440) Human Untagged Clone

CAT#: SC111221

MTHFD1L (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTHFD1L
• Synonyms: dJ292B18.2; FTHFSDC1; MTC1THFS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015440, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCACGCGTCTGCCGCTCGTCCTGCGCCAGCTCCGCCGCCCGCCCCAGCCCCCGGGCCCTCCGCGCC
  GCCTCCGTGTGCCCTGTCGCGCTAGCAGCGGCGGCGGCGGAGGCGGCGGCGGTGGCCGGGAGGGCCTGCT
  TGGACAGCGGCGGCCGCAGGATGGCCAGGCCCGGAGCAGCTGCAGCCCCGGCGGCCGAACGCCCGCGGCG
  CGGGACTCCATCGTCAGAGAAGTCATTCAGAATTCAAAAGAAGTTCTAAGTTTATTGCAAGAAAAAAACC
  CTGCCTTCAAGCCGGTTCTTGCAATTATCCAGGCAGGTGACGACAACTTGATGCAGGAAATCAACCAGAA
  TTTGGCTGAGGAGGCTGGTCTGAACATCACTCACATTTGCCTCCCTCCAGATAGCAGTGAAGCCGAGATT
  ATAGATGAAATCTTAAAGATCAATGAAGATACCAGAGTACATGGCCTTGCCCTTCAGATCTCTGAGAACT
  TGTTTAGCAACAAAGTCCTCAATGCCTTGAAACCAGAAAAAGATGTGGATGGAGTAACAGACATAAACCT
  GGGGAAGCTGGTGCGAGGGGATGCCCATGAATGTTTTGTTTCACCTGTTGCCAAAGCTGTAATTGAACTT
  CTTGAAAAATCAGGTGTCAACCTAGATGGAAAGAAGATTTTGGTAGTGGGGGCCCATGGGTCTTTGGAAG
  CTGCTCTACAATGCCTGTTCCAGAGAAAAGGGTCCATGACAATGAGCATCCAGTGGAAAACACGCCAGCT
  TCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGCCAGAAGAGATTCCCCTTACT
  TGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATGACTTCCTGTCAGGGAAGGTTGGGTGTGGCT
  CTCCAAGAATACATTTTGGTGGACTCATTGAGGAAGATGATGTGATTCTCCTTGCTGCAGCTCTGCGAAT
  TCAGAACATGGTCAGTAGTGGAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGGTGGAGACTTCACTGC
  TTGAAACTTCAGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGACAAACTCCAAAAGCTG
  TGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAAAGCAAAGCCAA
  AGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAATACGTCTTAGTTGCTGGGATC
  ACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCATCGGGCTTGTGCAGGCTCTGACCGCACACC
  TGAATGTCAACTCCTTTGCCTGCTTGAGGCAGCCTTCCCAAGGACCGACGTTTGGAGTGAAAGGAGGAGC
  CGCGGGTGGTGGATATGCCCAGGTCATCCCCATGGAGGAGTTCAACCTTCACTTGACTGGAGACATCCAC
  GCCATCACCGCTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGATTCTTCATGAAAACACGCAAA
  CAGATAAGGCTCTGTATAATCGGCTGGTTCCTTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAGCT
  TGCTCGGCTAAAAAAACTGGGAATAAATAAGACTGATCCGAGCACACTGACAGAAGAGGAAGTGAGTAAA
  TTTGCCCGTCTCGACATCGACCCATCTACCATCACGTGGCAGAGAGTATTGGATACAAATGACCGATTTC
  TACGAAAAATAACCATCGGGCAGGGAAACACAGAGAAGGGCCATTACCGGCAGGCGCAGTTTGACATCGC
  AGTGGCCAGCGAGATCATGGCGGTGCTGGCCCTGACGGACAGCCTCGCAGACATGAAGGCACGGCTGGGA
  AGGATGGTGGTGGCCAGTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGGGGGTGACAGGTGCTT
  TGACAGTTTTGATGAAAGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGGGACACCTGTGTTCGT
  GCATGCGGGCCCTTTTGCTAACATTGCTCACGGCAACTCTTCAGTGTTGGCTGATAAAATTGCCCTGAAA
  CTGGTTGGTGAAGAAGGATTTGTAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCT
  TCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCT
  GAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAGAAC
  ATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTC
  CCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGC
  AAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGAC
  TTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTC
  CAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCCTGA
  GGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACC
  CACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACG
  TCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCC
  CACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGCTTGTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015440 unedited
  GGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCTTTGGAA
  GCTGCTCTACAATGCCTGTTCCAGAGAAAGGGTCCATGACAATGAGCATCCAGTGGAAAA
  CACGCCAGCTTCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGC
  CAGAAGAGATTCCCCTTACTTGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATG
  ACTTCCTGTCAGGGAAGGTTGGGTGTGGCTCTCCAAGAATACATTTTGGTGGACTCATTG
  AGGAAGATGATGTGATTCTCCTTGCTGCAGCTCTGCGAATTCAGAACATGGTCAGTAGTG
  GAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGGTGGAGACTTCACTGCTTGAAACTTC
  AGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGACAAACTCCAAAAGCTG
  TGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAAA
  GCAAAGCCAAAGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAAT
  ACGTCTTAGTTGCTGGGATCACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCA
  TCGGGCTTGTGCANGCTCTGACCGCACACCTGAATGTCAACTCCTTTGCCTGCTTGAGGC
  AGCCTTCCCAAGGACCGACGTTTGGAGTGAAAGGAGGAGCCGCGGGTGGTGGATATGNCC
  CAGTCATCCCCATGGGAGAGNTCACCCTTCACTTGACTGGAGACATNCACGCCATCACCG
  CTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015440 unedited
  TTGTACATTATGNACCGCGCCGCATTCTGANGATCGGTTTTTTTTTTTTTTTTTTTTGGT
  TTTCTAGATCCTGTTTATTTTCAGTTCAAACCAAAGTAGAAAACTTGAGAGTGGAAAATG
  TTACCTTTTAGTTCACACTCCTAATCCCTTAGTCCCCATAAAATAAACATTCTAAAGTGT
  AAGCAGTAGAAATAATGGAAACTCCACAGAAACAGAAATAAATTAGTTTCTTTCAGTCTT
  GGTGGAGGTCCTTTTGCCGAACACCATACTCCACTGTGAACAGAATTCATCTTGAACGAA
  GAAGAAATCTTTGGCCTATTTCACCACGTCTCCAGCATTGCATAACAGACATTTTTCAAA
  TTCAGTTTCTTCTCCAACTGCAGCAAAAAGGCAAAGAGTAGTCTGTTTCAGGAGTCTGCA
  TCGGGTCCTGTGAGAGCCTTGTCCACTTAGAACAAGCCTTTAACTTGTTCTGTTTCGGTA
  TCAAGATCTATGTCATAAAAGCAGGGCCGGGTGGGCAGTCCTGGCATGGTGCTCATCGTT
  CCGACCAAAGGGTAAATGAACCCAGCGCCTATGCTGGCCCGGACGTCACTGATAGGTAAG
  ATGAAGTCCCTTGGCACACCTTTTTTGTCAGGTTGGTGAGATAGAGAAAGGTGGGTCTTT
  GCCATGCAGATGGGCAAATTTCCAAAACCCTGTTGAGTGTAACGATCTATTTTGGCTTGT
  GCCTCAGGAGAGAGTTCAATATCTTTGGCTCCATAGACAGCCTGAGCAATGGTCCTTATC
  TTGTCCACAATTGGNAACCTGAACATCATACAGGAACTGGNAATCGCTTCTTTTACTCGC
  AGCCTCTCTCACAGCCCGAGCCAAGTCCACCGATTCTTTTCCACCACGGACCAGTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015440
• ORF Size: 2202 bp
• Insert Size: 2750
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_015440.1, NP_056255.1
• RefSeq Size: 3487
• RefSeq ORF: 2202
• Locus ID: 25902
• Domains: FTHFS
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
• Gene Summary: The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.