Amyloid Precursor Protein (APP) (NM_000484) Human Untagged Clone

CAT#: SC111589

APP (untagged)-Human amyloid beta (A4) precursor protein (APP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APP
• Synonyms: AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; preA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000484 edited
  CTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAG
  CACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAG
  CAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCG
  ATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTA
  CCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGA
  CTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAA
  ACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTG
  CAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGG
  GGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGT
  GAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGG
  ATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAG
  AAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGA
  GGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGAT
  GCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGG
  AGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAA
  GAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAA
  CCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACA
  GAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGC
  CGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTT
  TACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTG
  TGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGAT
  CCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAG
  ACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCC
  AAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCA
  AAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAA
  TCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGA
  GTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTG
  CAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCA
  GAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCC
  AAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGC
  ATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGAT
  GAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATG
  ATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACG
  AAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCG
  TGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTT
  GATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAAT
  ATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGA
  TATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGT
  GCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTG
  GTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCC
  GCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCA
  ACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTT
  GGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAG
  AAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAA
  GTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACAT
  TTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATC
  AAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTG
  TATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATC
  GATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCT
  TTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGA
  GATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGAT
  ATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTA
  GGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGA
  AAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTG
  GTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCAT
  TGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000484 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTGAGCCCCGCCGCCGCGC
  TCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGG
  GGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCAC
  TCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTG
  CTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGC
  CTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTC
  CAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAA
  GGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAA
  GCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGNGCCGCAAGCAGTGCAAGACC
  CATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTT
  CTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGGATGTNTNGCGAAACTC
  ATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATG
  ACTACGGCATGTTGCTGCCCTGCGGNATTGACAGTNCCGAGGTGACAGTTCTGGGCGCCT
  GCCCCTGCCTGNAGAAAGCGACATGCGGCTCCCGCTGTGCGGCACGAGATGCCCACGCAG
  CCTGCCGCCCCGTCCTACTCTCCCTTACCCCGTTCTCTCCCCCTCCCCCCCCCTCCTCCC
  GCCCCCCCCCCGTCTTTATTACGCGTCCTCCTCTCTCCCTGCATCGTCGTTCCTGGCTGT
  ACCCCCCCATCTCACCCACTTCGGCCTTCCTCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000484 unedited
  CCATCGNGANNGATGGCAACTTCCCAGGTTCAGGAAAGCACTGGGGCAGGGTCACAGGGC
  TGCCACCCGGGTTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT
  TTTTTTTTTTTTTTTTTATTTTATTTAATTTATTTATGTAATACAGTGTAGAAAGCGATC
  ATGTCATAAGCAATGATTCTGTACAATCATCCTGCAAAAAATTGTTTTGGAGAATTCTTG
  GTAATTGAAGACCAGCAGAGCACCCCTCCCCACCCGCCCCGTAAAAGTGCTTACAATGAA
  CAGGGATTCTTTTCTTTATCAAAGACCCAAAGATACGTGGACAAAAAAAGAAAAGCTTGA
  AGTCTCAATGCCTAATGTGTGCACATAAAACAGGCACGAAGAAACAAACGTGTGTATCCT
  CTTAATTCCTATATCACAAATATAGCAGAAGCAGCAATCTGTACAGTAAAATGCAGTCAT
  GGAAAAAAAATCTCTCTAAAGCATCTGAAATACTTAAAAATGTTTAACTTTAAAATGCAT
  AGTGATCAGGAAAGGAATACTTAGGCAAGAGAAGCAGCTGAACTCCCACGTTCACATGAA
  GCATCCCCCATCGATTCTTAAAGCATATGTAAAGTAAGACTTAATTGGGTCACACACCAC
  AAGAATAATATACAACTGGCTAAGGGGCTATGTGATAAATAATCAGGAAAGAATCCTTTC
  CTGCCCTAGTTTGATACAGCTAAATTCTTTACGGTCACCAAACCCTTTTTTTATTGTTTT
  TTGAGCCCAATTGTAAGGGGGGGTGGAATCCCTTCCGGGTGTGTGGTTCCATTCAGGGTT
  AGCTTTTTTTTGTTTTCACTCCCTCTGCCCATGGCGNTTTTGTGTAACCCTAAAAAGGGC
  GTTTTCTTCCCCCTTTTTCTTTGAGGGCCCAAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000484
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000484.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000484.2, NP_000475.1
• RefSeq Size: 3641 bp
• RefSeq ORF: 2313 bp
• Locus ID: 351
• Cytogenetics: 21q21.3
• Domains: Beta-APP, KU, A4_EXTRA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease
• Gene Summary: 'This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as PreA4 770).