PPEF1 (NM_006240) Human Untagged Clone

CAT#: SC111671

PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPEF1
• Synonyms: PP7; PPEF; PPP7C; PPP7CA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111671 sequence for NM_006240 edited (data generated by NextGen Sequencing)
  ATGGGATGCAGCAGTTCTTCAACGAAAACCAGGAGATCTGACACATCACTGAGAGCTGCG
  TTGATCATCCAGAACTGGTACCGAGGTTACAAAGCTCGACTGAAGGCCAGACAACACTAT
  GCCCTCACCATCTTCCAGTCCATCGAATATGCTGATGAACAAGGCCAAATGCAGTTATCC
  ACCTTCTTTTCCTTCATGTTGGAAAACTACACACATATACATAAGGAAGAGCTAGAATTA
  AGAAATCAGTCTCTTGAAAGCGAACAGGACATGAGGGATAGATGGGATTATGTGGACTCG
  ATAGATGTCCCAGACTCCTATAATGGTCCTCGGCTACAATTTCCTCTCACTTGTACGGAT
  ATTGATTTACTTCTTGAGGCCTTCAAGGAACAACAGATACTTCATGCCCATTATGTCTTA
  GAGGTGCTATTTGAAACCAAGAAAGTCCTGAAGCAAATGCCGAATTTCACTCACATACAA
  ACTTCTCCCTCCAAAGAGGTAACAATCTGTGGTGATTTGCATGGGAAACTGGATGATCTT
  TTTTTGATCTTCTACAAGAATGGTCTCCCCTCAGAGAGGAACCCGTATGTTTTTAATGGT
  GACTTTGTAGATCGAGGAAAGAATTCCATAGAGATCCTAATGATCCTGTGTGTGAGTTTT
  CTTGTCTACCCCAATGACCTGCACTTGAACAGAGGGAACCACGAAGATTTTATGATGAAT
  CTGAGGTATGGCTTCACGAAAGAAATTTTGCATAAATATAAGCTACATGGAAAAAGAATC
  TTACAAATCTTGGAAGAATTCTATGCCTGGCTCCCAATCGGTACAATCGTTGACAATGAA
  ATCCTGGTCATCCATGGTGGGATATCAGAGACCACAGACTTGAATTTACTCCACCGTGTA
  GAGAGGAACAAGATGAAATCTGTGCTGATACCACCAACGGAAACAAACAGAGACCATGAC
  ACTGACTCGAAGCACAATAAAGTAGGTGTGACTTTTAATGCACATGGAAGAATCAAAACA
  AATGGATCTCCTACTGAACACTTAACAGAGCATGAATGGGAACAGATTATTGATATTCTG
  TGGAGTGATCCCAGAGGCAAAAATGGCTGTTTTCCAAATACGTGCCGAGGAGGGGGCTGC
  TATTTTGGACCAGATGTTACTTCCAAGATTCTTAATAAATACCAGTTGAAGATGCTCATC
  AGGTCTCATGAATGTAAGCCCGAAGGGTATGAAATCTGTCATGATGGGAAGGTGGTGACT
  ATATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAATCGAGGAGCTTACATCAAACTA
  TGTTCTGGTACAACTCCTCGATTTTTCCAGTACCAAGTAACTAAAGCAACGTGCTTTCAG
  CCTCTTCGCCAAAGAGTGGATACTATGGAAAACAGCGCCATCAAGATATTAAGAGAGAGA
  GTGATTTCACGAAAAAGTGACCTTACTCGTGCTTTCCAACTTCAAGACCACAGAAAATCA
  GGAAAACTTTCTGTGAGCCAGTGGGCTTTTTGCATGGAGAACATTTTGGGGCTGAACTTA
  CCATGGAGATCCCTCAGTTCGAATCTGGTAAACATAGACCAAAATGGAAACGTTGAATAC
  ATGTCCAGCTTCCAGAATATCCGCATTGAAAAACCTGTACAAGAGGCTCATTCTACTCTA
  GTTGAAACTCTGTACAGATACAGATCTGACCTGGAAATCATATTTAATGCCATTGACACT
  GATCACTCAGGCCTGATCTCCGTGGAAGAATTTCGTGCCATGTGGAAACTTTTTAGTTCT
  CACTACAATGTTCACATTGATGATTCCCAAGTCAATAAGCTTGCCAACATAATGGACTTG
  AACAAAGATGGAAGCATTGACTTTAATGAGTTTTTAAAGGCTTTCTATGTAGAGCATAGA
  TATGAAGACTTGATGAAACCTGATGTCACCAACCTTGGCTAA
  
  Clone variation with respect to NM_006240.2
  1913 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006240 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGGTTTCCTTCC
  CTCCCTCCTGTGTATTCCTCATTAGAATCTATGACTGAAGAGGATCGGCTAAGAGTGGTT
  CCTCGCAGCTTAAAGGGAGGCACTTTTCACACTCTGTCTTAAAATCAGAAGTTGAATTCA
  TGAACACATATGATTTAGATAGAAGTCATGGGATGCAGCAGTTCTTCAACGAAAACCAGG
  AGATCTGACACATCACTGAGAGCTGCGTTGATCATCCAGAACTGGTACCGAGGTTACAAA
  GCTCGACTGAAGGCCAGACAACACTATGCCCTCACCATCTTCCAGTCCATCGAATATGCT
  GATGAACAAGGCCAAATGCAGTTATCCACCTTCTTTTCCTTCATGTTGGAAAACTACACA
  CATATACATAAGGAAGAGCTAGAATTAAGAAATCAGTCTCTTGAAAGCGAACAGGACATG
  AGGGATAGATGGGATTATGTGGACTCGATAGATGTCCCAGACTCCTATAATGGTCCTCGG
  CTACAATTTCCTCTCACTTGTACGGATATTGATTTACTTCTTGAGGCCTTCAAGGAACAA
  CAGATACTTCATGCCCATTATGTCTTAGAGGTGCTATTTGAAACCAAGAAAGTCCTGAAG
  CAAATGCCGAATTTCACTCACATACAAACTTCTCCCTCCAAAGAGGTAACCATCTGTGGT
  GATTTGCATGGGAAACTGGATGATCTTTTTTTTGATCTTCTACAGAAATGGTCTCCCCTC
  AGAGAGGAACCCGTATGTTTTTATGGTGACNTTGTAGATCGAGGAAAGATCCATAGAGAT
  CTATGATCCTGGNGTGAGTTTCTGTCTACCCATGACCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006240 unedited
  TGGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAGTTTAAAGATG
  GAAAGACTTTATTCCATGAATAACCTGGTATTCCTCCTGTCTCCCTCAGTCACTCACAGA
  TGTCAAGCTTAGTAGTTTTCTCTTGTGCTGTATGAGTTCCTTTCAGGATGTTTTCAAACC
  GCTCTGGCCTCCTGGTTAGGTGGCACTCACGACAACACCACTTAAGGGTCCAACCCAGTT
  TCTCACTGGCAATATGTAGGTACTAATGCTGAAACCAAATCCTATTACTGACATTTAACC
  TAATCTTACCAATAACAAGCTTGCTAGGGACTTCCAAAACCCATACTATGCATCTGTTTG
  TGGGATGAATTGGGATCTGCTTTTCTCTGCTACTGACTATGAATTTCAGGGGTGTTGGAA
  AGGACTGTACTTGTGATTTGGGCCTAGCTGTTTCAGGGAGCCTGAGGGAAGCTCTCATTT
  GTGTTTAGCCAAGGTTGTGACATCANGTTTCATCAAGTCTTCATATCTATGCTCTACATA
  GAAAAGCCCTTAAACTCATTAAAGTCAATGCTTCCATCTTTGNTCAAGTCCATTTATGTN
  GGCAGCTTATTGACTTGGGAATCATCAATGTGAACATTGTANTGAGAACTAAAAAAGTTC
  ACATGGCACGAAATTCTTCCCACGAGATCAGGCCTGANTGATCAGTGTCAATGGCATTAA
  TATGATTTCCCAGTCAGATCTGTATCTGACAGAGTTTCAACTAGAGTAGAATGACCCTNT
  TGTACAAGTTTTCAATGCGGAATTTCTTGAAAGCTGGCAATGATTCAACGTTTCCATTTT
  GGCCATTGTTACCAAATTCAACTGAAGGATCTCCTGGAAGTTCACCCCAAATAGTTTTCA
  TGCAAAAACCCCCTGCTCCCCAAAGTTTTCTGATTTCTTGGGCTTTGAATGGAAGCCCAA
  TAGGGCATTTTTCGGAAACACTCTTTTTAATATTGAAGGCCCGGTTCCTAAATCACCTCT
  GGCGAAAAGCTAAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006240
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006240.2, NP_006231.2
• RefSeq Size: 2873 bp
• RefSeq ORF: 1962 bp
• Locus ID: 5475
• Cytogenetics: Xp22.13
• Domains: IQ, EFh, Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the predominant isoform (1).