PSMD13 (NM_002817) Human Untagged Clone

CAT#: SC111679

PSMD13 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD13
• Synonyms: HSPC027; p40.5; Rpn9; S11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111679 sequence for NM_002817 edited (data generated by NextGen Sequencing)
  ATGAAGGACGTACCGGGCTTCCTACAGCAGAGCCAGAACTCCGGGCCCGGGCAGCCCGCT
  GTGTGGCACCGTCTGGAGGAGCTCTACACGAAGAAGTTGTGGCATCAGCTGACACTTCAG
  GTGCTTGATTTTGTGCAGGATCCGTGCTTTGCCCAAGGAGATGGTCTCATTAAGCTTTAT
  GAAAACTTTATCAGTGAATTTGAACACAGGGTGAACCCTTTGTCCCTCGTGGAAATCATT
  CTTCATGTAGTTAGACAGATGACTGATCCTAATGTGGCTCTTACTTTTCTGGAAAAGACT
  CGTGAGAAGGTGAAAAGTAGTGATGAGGCAGTGATCCTGTGTAAAACAGCAATTGGAGCT
  CTAAAATTAAACATCGGGGACCTACAGGTTACAAAGGAAACAATTGAAGATGTTGAAGAA
  ATGCTCAACAACCTTCCTGGTGTGACATCGGTTCACAGTCGTTTCTATGATCTCTCCAGT
  AAATACTATCAAACAATCGGAAACCACGCGTCCTACTACAAAGATGCTCTGCGGTTTTTG
  GGCTGTGTTGACATCAAGGATCTACCAGTGTCTGAGCAGCAGGAGAGAGCCTTCACGCTG
  GGGCTAGCAGGACTTCTCGGCGAGGGAGTTTTTAACTTTGGAGAACTCCTCATGCACCCT
  GTGCTGGAGTCCCTGAGGAATACTGACCGGCAGTGGCTGATTGACACCCTCTATGCCTTC
  AACAGTGGCAACGTAGAGCGGTTCCAGACTCTGAAGACTGCCTGGGGCCAGCAGCCTGAT
  TTAGCAGCTAATGAAGCCCAGCTTCTGAGGAAAATTCAGTTGTTGTGCCTCATGGAGATG
  ACTTTCACACGACCTGCCAATCACAGACAACTCACTTTTGAAGAAATTGCCAAAAGTGCT
  AAAATCACAGTGAATGAGGTGGAGCTTCTGGTGATGAAGGCCCTTTCGGTGGGGCTGGTG
  AAAGGCAGTATAGACGAGGTGGACAAACGAGTCCACATGACCTGGGTGCAGCCCCGAGTG
  TTGGATTTGCAACAGATCAAGGGAATGAAGGACCGCCTGGAGTTCTGGTGCACGGATGTG
  AAGAGCATGGAGATGCTGGTGGAGCACCAGGCCCATGACATCCTCACCTAG
  
  Clone variation with respect to NM_002817.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002817 unedited
  TCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCGGTGCTGACATCCCGGTTGTTCT
  TCTGTGCCGGGGGTCTTCCTGCTGTCATGAAGGACGTACCGGGCTTCCTACAGCAGAGCC
  AGAACTCCGGGCCCGGGCAGCCCGCTGTGTGGCACCGTCTGGAGGAGCTCTACACGAAGA
  AGTTGTGGCATCAGCTGACACTTCAGGTGCTTGATTTTGTGCAGGATCCGTGCTTTGCCC
  AAGGAGATGGTCTCATTAAGCTTTATGAAAACTTTATCAGTGAATTTGAACACAGGGTGA
  ACCCTTTGTCCCTCGTGGAAATCATTCTTCATGTAGTTAGACAGATGACTGATCCTAATG
  TGGCTCTTACTTTTCTGGAAAAGACTCGTGAGAAGGTGAAAAGTAGTGATGAGGCAGTGA
  TCCTGTGTAAAACAGCAATTGGAGCTCTAAAATTAAACATCGGNGACCTACAGGTTACAA
  AGGAAACAATTGAAGATGTTGAAGAAATGCTCAACAACCTTCCTGGTGTGACATCGGTTC
  ACAGTCGTTTCTATGATCTCTCCAGTAAATACTATCAAACAATCGGAAACCACGCGTCCT
  ACTACAAGATGCTCTGCGGTTNTTGGGCTGTGTTGACATCAAGGATCTACCAGTGTCTGA
  GCAGCAGGAGAGAGCCTTCACGCTGGGGCTAGCAGGACTTNCTCGCGAGGGAGTTNTAAA
  ACTTGAGAACTCTCATGCACCCTGTGCTGNAGTCCCTGAGGATACTGACCGCAATGGGCT
  GATGACACCTCTATGCCTTCACAGTGGCACGTAAAACGGTCCAGATCTGAGACTGCCTGG
  GCCANCAGCTGATTTACACTATGAAGCCAGCTCTGGGAAAATCAGTNGTNGGCCTATGAG
  ATACTTCACACACTGCATACAGACACTACTTTGAGAAATGCCAAGGCTAATCCAGGATGA
  GTGACTCTGTGATAAGCCTCGGGGGCTGGGAAGCNTAACAGGGACACAGTCATACTGTGC
  CCCCAGTGATTGAAACAGGATAGCCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002817 unedited
  GCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAACAAAGCAGAGGGGTTTATTA
  TAGGAACATTCTCAAACCGCAACGGAAAAGATGTCCGTACAGGTGGATGGGGATGGAGAT
  CCACCTCGGAGTACACAGACTTCAGGGGGCCTCCTGCCTGGCACGTTCTTTCTCTCCCGT
  ATCACCTAAGACCCTGAGACCCCCACCCTCTGCAGGAGAGACCCACAAAGAAGCCTCCTC
  CCTGTGGCCTGGCTCCCATCAAGGACAGTCCTGTTTTTAGAGCAAGAACAGTCTGTACTT
  CAGACAGGATCCCAACCCCCACCCAAATTCAATGTCGACCGTCTGAGCAGCCAGCTTCAT
  TGGCTGCAAACGCCTCTCTCAGGTGAGTCAAAGGAGACACGACGGGGAACCAGGGGGCCC
  TAGGTGAGGATGTCATGGGCCTGGTGCTCCACCAGCATCTCCATGCTCTTCACATCCGTG
  CACCAGAACTCCAGGCGGTCCTTCATTCCCTTGATCTGTTGCAAATCCAACACTCGGGGC
  TGCACCCAGGTCATGTGGACTCGTTTGTCCACCTCGTCTATACTGCCTTTCACCAGCCCC
  ACCGAAAGGGCCTTCATCACCAGAAGCTCCACCTCATTCACTGTGATTTTAGCACTTTTG
  GCAATTTCTTCAAAAGTGAGTTGCCTGTGATAGGCAGGTCGTGTGAAAGTCATCTCCATG
  AGCACAACACCCGATTTACCCTCAAAAGCTGGGCTTCATTAACCTGCTAAATAAGCTGCT
  GCCCCCAGGCGTCTTAAAAGTCTGGAACCCCCTACGTTGCCCTGCTGAAAGCTTAAGGGT
  GCAATCACCCCTGCCGTCATTATTCTCAGGACTCCCCCAAGGCGCTGAGAGTCCCCCAAC
  TAAAACTCCCTCCCCATAATCCGCACCCCACCGGAAGCTCTCCCGGCCGTCAAACCGAGA
  AACCTTAGGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002817
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002817.2, NP_002808.2
• RefSeq Size: 1587 bp
• RefSeq ORF: 1131 bp
• Locus ID: 5719
• Cytogenetics: 11p15.5
• Domains: PCI
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).