RHD (NM_016124) Human Untagged Clone

CAT#: SC111686

RHD (untagged)-Human Rh blood group, D antigen (RHD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHD
• Synonyms: CD240D; DIIIc; RH; Rh4; RH30; RHCED; RhDCw; RHDel; RHDVA(TT); RhII; RhK562-II; RhPI; RHPII; RHXIII
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111686 sequence for NM_016124 edited (data generated by NextGen Sequencing)
  ATGAGCTCTAAGTACCCGCGGTCTGTCCGGCGCTGCCTGCCCCTCTGGGCCCTAACACTG
  GAAGCAGCTCTCATTCTCCTCTTCTATTTTTTTACCCACTATGACGCTTCCTTAGAGGAT
  CAAAAGGGGCTCGTGGCATCCTATCAAGTTGGCCAAGATCTGACCGTGATGGCGGCCATT
  GGCTTGGGCTTCCTCACCTCGAGTTTCCGGAGACACAGCTGGAGCAGTGTGGCCTTCAAC
  CTCTTCATGCTGGCGCTTGGTGTGCAGTGGGCAATCCTGCTGGACGGCTTCCTGAGCCAG
  TTCCCTTCTGGGAAGGTGGTCATCACACTGTTCAGTATTCGGCTGGCCACCATGAGTGCT
  TTGTCGGTGCTGATCTCAGTGGATGCTGTCTTGGGGAAGGTCAACTTGGCGCAGTTGGTG
  GTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCAACCTGAGGATGGTCATCAGTAATATC
  TTCAACACAGACTACCACATGAACATGATGCACATCTACGTGTTCGCAGCCTATTTTGGG
  CTGTCTGTGGCCTGGTGCCTGCCAAAGCCTCTACCCGAGGGAACGGAGGATAAAGATCAG
  ACAGCAACGATACCCAGTTTGTCTGCCATGCTGGGCGCCCTCTTCTTGTGGATGTTCTGG
  CCAAGTTTCAACTCTGCTCTGCTGAGAAGTCCAATCGAAAGGAAGAATGCCGTGTTCAAC
  ACCTACTATGCTGTAGCAGTCAGCGTGGTGACAGCCATCTCAGGGTCATCCTTGGCTCAC
  CCCCAAGGGAAGATCAGCAAGACTTATGTGCACAGTGCGGTGTTGGCAGGAGGCGTGGCT
  GTGGGTACCTCGTGTCACCTGATCCCTTCTCCGTGGCTTGCCATGGTGCTGGGTCTTGTG
  GCTGGGCTGATCTCCGTCGGGGGAGCCAAGTACCTGCCGGGGTGTTGTAACCGAGTGCTG
  GGGATTCCCCACAGCTCCATCATGGGCTACAACTTCAGCTTGCTGGGTCTGCTTGGAGAG
  ATCATCTACATTGTGCTGCTGGTGCTTGATACCGTCGGAGCCGGCAATGGCATGATTGGC
  TTCCAGGTCCTCCTCAGCATTGGGGAACTCAGCTTGGCCATCGTGATAGCTCTCACGTCT
  GGTCTCCTGACAGGTTTGCTCCTAAATCTTAAAATATGGAAAGCACCTCATGAGGCTAAA
  TATTTTGATGACCAAGTTTTCTGGAAGTTTCCTCATTTGGCTGTTGGATTTTAA
  
  Clone variation with respect to NM_016124.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016124 unedited
  GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTGGTGCTGGT
  GGAACCCCTGCACAGAGACGGACACAGGATGAGCTCTAAGTACCCGCGGTCTGTCCGGCG
  CTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTCTCATTCTCCTCTTCTATTTTTT
  TACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATCCTATCAAGTTGG
  CCAAGATCTGACCGTGATGGCGGCCATTGGCTTGGGCTTCCTCACCTCGAGTTTCCGGAG
  ACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGC
  AATCCTGCTGGACGGCTTCCTGAGCCAGTTCCCTTCTGGGAAGGTGGTCATCACACTGTT
  CAGTATTCGGCTGGCCACCATGAGTGCTTTGTCGGTGCTGATCTCAGTGGATGCTGTCTT
  GGGGAAGGTCAACTTGGCGCAGTTGGTGGTGATGGTGCTGGTGGAGGTGACAGCTTTAGG
  CAACCTGAGGATGGTCATCAGTAATATCTTCAACACAGACTACCACATGAACATGATGCA
  CATCTACGTGTTCGCAGCCTATTTTGGGCTGTCTGTGGCCTGGTGCCTGCCAAAGCCTCT
  ACCCGAGGGAACGGAGGATAAAGATCAGACAGCAACGATACCCAGTTTGTCTGCCATGCT
  GGGCGCCCTCTTCTTGTGGATGTTCTGGCCAAGTTTCAACTCTGCTCTGCTGAGAAGTCC
  AATCGAAGGGAGAATGCCGTGTTCACACCTACTATGCTGTAGCAGTCAGCGTGGTGACAG
  CCATCTCAGGGTCATCCTTGGCTCACCCNCAGGGNAAGATCAGCAGACTATGTGCACAGT
  GCGTGTTGGCAAGAGCGTGGCTGTGGNTACCTCGTGTCACCTGATCCCTTCTNCGTGGCT
  NGCCATGGNGCTGGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016124 unedited
  AAACAAGGAAAACACCANACACATTCCCCCCCCCTTTTTACTTGNNACGCGGCCGCATTT
  NANGATCGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT
  TTTTTTTTTTTATGGGGAACATGGCTGTATTTTATTGGTGAATTCTACAGCGCATAAAAA
  ATGGGGAAATTTTCCTCAAAAAGGGCAAAAAAAGGATTCAACTCCATTTTCTTTGACTCC
  AGGGCCTGGGCAAACATTGGAAACTTTGCTGTCATAAGCGTTTCTCACCTCCAAATGCCG
  GCAACAGTGAAAGGAATTTGTCTTGTTTTTAAACAGGCCTTGTTTTTTTTGGATGCTTTT
  GCTTAAAATCCAACCTCCAAATGAGGAAACTTCCAAAAAACTTGGTCCTCAAAATATTTA
  ACCTCATGAGGGGCTTTCCATTTTTTAAAATTTAGGAACAAACCTGTCCGGAGACCAAAC
  GTGAAATCTTTCACCACGGCCAAGCTGATTTCCCCAATGTTGGGGAGGCCCTTGAAACCA
  ATCCTGCCATTGGCCGGCTCCAACGGGTTCAAACACCCCCCCAACAATGTAACAAATCTT
  CTCAAGCACAACCAACAGGTTCAACTTGAACCCCTTTATGGAACCCGCGGGGGAAATCCC
  CCGATTTTGGTTCCAAAACCCCCGGAGAGACTTTGGGTTCCCCCGCGGGAAATTCCCCCC
  ACCCCAAACACCCACCCCCTTGGCCACCCCCCCGTAAAAGGTGACCGGTTACAACAAGTA
  TCCTCCCAGGTCCCCTTTTTTGGAAAACCCGATTTGTGAAAAAAAAGCCTGGTGGAATTT
  CCCTGGGGGGGAGCCACACGGAATCCTCTTAAATGGGTTTACCCACTCTATTTGTTAAAA
  TTAATAGGGGTTTAAACACGGCTTTTTCTTTTCTCATGGACCCCCCCCACAAAAATAATT
  CAACCCTGGCCAAAATCCCCCACTAAACGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016124
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016124.2, NP_057208.2
• RefSeq Size: 2709 bp
• RefSeq ORF: 1254 bp
• Locus ID: 6007
• Cytogenetics: 1p36.11
• Domains: Ammonium_transp
• Protein Families: Transmembrane
• Gene Summary: 'The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.