TAZ (NM_000116) Human Untagged Clone

CAT#: SC111998

TAZ (untagged)-Human tafazzin (TAZ), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAZ
• Synonyms: BTHS; CMD3A; EFE; EFE2; G4.5; LVNCX; Taz1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000116, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTCTGCACGTGAAGTGGCCGTTCCCCGCGGTGCCGCCGCTCACCTGGACCCTGGCCAGCAGCGTCG
  TCATGGGCTTGGTGGGCACCTACAGCTGCTTCTGGACCAAGTACATGAACCACCTGACCGTGCACAACAG
  GGAGGTGCTGTACGAGCTCATCGAGAAGCGAGGCCCGGCCACGCCCCTCATCACCGTGTCCAATCACCAG
  TCCTGCATGGACGACCCTCATCTCTGGGGGATCCTGAAACTCCGCCACATCTGGAACCTGAAGTTGATGC
  GTTGGACCCCTGCAGCTGCAGACATCTGCTTCACCAAGGAGCTACACTCCCACTTCTTCAGCTTGGGCAA
  GTGTGTGCCTGTGTGCCGAGGAGCAGAATTTTTCCAAGCAGAGAATGAGGGGAAAGGTGTTCTAGACACA
  GGCAGGCACATGCCAGGTGCTGGAAAAAGAAGAGAGAAAGGAGATGGCGTCTACCAGAAGGGGATGGACT
  TCATTTTGGAGAAGCTCAACCATGGGGACTGGGTGCATATCTTCCCAGAAGGGAAAGTGAACATGAGTTC
  CGAATTCCTGCGTTTCAAGTGGGGAATCGGGCGCCTGATTGCTGAGTGTCATCTCAACCCCATCATCCTG
  CCCCTGTGGCATGTCGGAATGAATGACGTCCTTCCTAACAGTCCGCCCTACTTCCCCCGCTTTGGACAGA
  AAATCACTGTGCTGATCGGGAAGCCCTTCAGTGCCCTGCCTGTACTCGAGCGGCTCCGGGCGGAGAACAA
  GTCGGCTGTGGAGATGCGGAAAGCCCTGACGGACTTCATTCAAGAGGAATTCCAGCATCTGAAGACTCAG
  GCAGAGCAGCTCCACAACCACCTCCAGCCTGGGAGATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000116 unedited
  ATATTCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGA
  ATTCGGCACCAGAAGGGACCTCGGTCCAGTCCCCTGTTGCGCCGCGCCCCCGTCCGTCCG
  TGCGCGGGCCAGTCAGGGGCCAGTGTCTCGAGCGGTCGAGGTCGCAGACCTAGAGGCGCC
  CCACAGGCCGGCCCGGGGCGCTGGGAGCGCCGGCCGCGGGCCGGGTGGGGATGCCTCTGC
  ACGTGAAGTGGCCGTTCCCCGCGGTGCCGCCGCTCACCTGGACCCTGGCCAGCAGCGTCG
  TCATGGGCTTGGTGGGCACCTACAGCTGCTTCTGGACCAAGTACATGAACCACCTGACCG
  TGCACAACAGGGAGGTGCTGTACGAGCTCATCGAGAAGCGAGGCCCGGCCACGCCCCTCA
  TCACCGTGTCCAATCACCAGTCCTGCATGGACGACCCTCATCTCTGGGGGATCCTGAAAC
  TCCGCCACATCTGGAACCTGAAGTTGATGCGTTGGACCCCTGCAGCTGCAGACATCTGCT
  TCACCAAGGAGCTACACTCCCACTTCTTCAGCTTGAGCAAGTGTGTGCCTGTGTGCCGAG
  GAGATGGCGTCTACCAGAAGGNGATGGACTTCATTTTGGAGAAGCTCAACCATGGGGACT
  GGGTGCATATCTTCCCCAGAGGGAAAGTGAACATGAGTTCCGAATTCCTGCGNNTTCAGT
  GGGGAATCGGGCGCCTGATTGCTGAGTGTCATCTCAACCCCATCATCCTGCCCCTGTGGC
  ATGGNTCGATGAATGACGTCCTTNNCTAAAGTNNCGCCCTACTTCCCCCGCTTGGACAGG
  AAATACCTGTGCTGATCGGGAAGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000116 unedited
  NNNNNAATTACTTGNACCGCGCCGCATTCNANNGATCGATTTTTTTTTTTTTTTTTTTTT
  TTTTACAACAAGNCCAACACATATGCTTTATGGTTCTGTTTCAAAAACATGAAAACCCAG
  GCCCTAGGATTCAAGTGATGTTGAGAGGCAGAAAGGATGGAGAAGTTGTTGGCGCCAGCA
  TGGGGGCAAGCAGCCAGACCTCAGGCCCTCACTCTAGGCTCCACGTGGCTGGGCTTGACT
  TCTGGCCAAGGGCTGCTTTTCCCGCCTGCGGCCCACCTGAGGCCAGACCTTGCCTGAGAA
  AGGCCTGTGGTGGGGGGGAAGCTCAGGCAGAAGGAAGACGTGGCCAACGGCCATCCCTGA
  GGAGGTAGTGAGCAGCTCCTGGTTCGGCAAGGGCGGTGGGTAGGAGCCAGGCCGAGGAGT
  CTTAGGGTCCCCTCTCCACAGCTCTGGTGGGCCCCCTGCTCCCCTAGCCAACTGGTTGCT
  CACCTGCCTTCTATGCTCCCTCCTGAAGCCAGCCCCTGGGAGAGAACATCTGAAGGCAAG
  AAGGAAAGAGAGCCCTGCCTAAGCTTCTTCCTCCCTGCTTGGCCAAGTGGGAGAAGCAGC
  TGAGGTCCTTTGTGTAACTGAGGAAGCACGGAGGAGCTGGAATGCCTACCAGCTCGGAGA
  GGGCACTTGAGAGGGTCTATGAACAAATCTGTCTAAAAGCCACGTTTGAGCTAAAAGCAT
  CAGTCCATCCCTCAGCCCCAGCTCTGTGCGGGCCAAGAATCCAGAAGGCAGCAAGGCAGG
  CCTATCTCCCCAGCTGGAGGTGGTNTGTGGAGCTGCTCTGCCTGAGTCTCAGATGCTGGA
  ATTCCTCTTGAAAGAAGTCCGTCAGGGCTTTTCCGCACTCCACAGCCGACTTGGTCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000116
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000116.2, NP_000107.1
• RefSeq Size: 1904 bp
• RefSeq ORF: 879 bp
• Locus ID: 6901
• Cytogenetics: Xq28
• Domains: Acyltransferase
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: 'This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the major isoform (1).