AMY2B (NM_020978) Human Untagged Clone

CAT#: SC113058

AMY2B (untagged)-Human amylase, alpha 2B (pancreatic) (AMY2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMY2B
• Synonyms: AMY2; AMY3; HXA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113058 sequence for NM_020978 edited (data generated by NextGen Sequencing)
  ATGAAGTTCTTTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAAT
  ACACAACAAGGACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGCT
  CTTGAATGTGAGCGATATTTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACCA
  AATGAAAATGTTGCAATTCACAACCCTTTCAGACCTTGGTGGGAAAGATACCAACCAGTT
  AGCTATAAATTATGCACAAGATCTGGAAATGAAGATGAATTTAGAAACATGGTGACTAGA
  TGTAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATATGTCTGGTAAT
  GCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGAC
  TTTCCAGCAGTCCCATATTCTGGATGGGATTTTAATGATGGTAAATGTAAAACTGGAAGT
  GGAGATATCGAGAACTACAATGATGCTACTCAGGTCAGAGATTGTCGTCTGGTTGGTCTT
  CTTGATCTTGCACTGGAGAAAGATTATGTGCGTTCCAAGATTGCCGAATATATGAATCAT
  CTCATTGACATTGGTGTTGCAGGGTTCAGACTTGATGCTTCCAAGCACATGTGGCCTGGA
  GACATAAAGGCAATTTTGGACAAACTGCATAATCTAAACAGTAACTGGTTCCCTGCAGGA
  AGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGTGGTGAGCCAATTAAAAGCAGT
  GACTACTTTGGAAATGGCCGGGTGACAGAATTCAAGTATGGTGCAAAACTCGGCACAGTT
  ATTCGCAAGTGGAATGGAGAGAAGATGTCTTACCTAAAGAACTGGGGAGAAGGTTGGGGT
  TTCATGCCTTCTGACAGAGCACTTGTCTTTGTGGATAACCATGACAATCAACGAGGACAT
  GGGGCTGGAGGAGCCTCTATTCTTACCTTCTGGGATGCTAGGCTGTATAAAATGGCAGTT
  GGATTTATGCTTGCTCATCCTTATGGTTTTACACGAGTAATGTCAAGCTACCGTTGGCCA
  AGACAGTTTCAAAATGGAAACGATGTTAATGATTGGGTTGGGCCACCAAATAATAATGGA
  GTAATTAAAGAAGTTACTATTAATCCAGACACTACTTGTGGCAATGACTGGGTCTGTGAA
  CATCGATGGCGCCAAATAAGGAACATGGTTAATTTCCGCAATGTAGTGGATGGCCAGCCT
  TTTACAAACTGGTATGATAATGGGAGCAACCAAGTGGCTTTTGGGAGAGGAAACAGAGGA
  TTCATTGTTTTCAACAATGATGACTGGACATTTTCTTTAACTTTGCAAACTGGTCTTCCT
  GCTGGCACATACTGTGATGTCATTTCTGGAGATAAAATTAATGGCAATTGCACAGGCATT
  AAAATCTACGTTTCTGACGATGGCAAAGCTCATTTTTCTATTAGTAACTCTGCTGAGGAT
  CCATTTATTGCAATTCATGCTGAATCTAAATTATAA
  
  Clone variation with respect to NM_020978.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020978 unedited
  GTTCAGATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACTCAACTA
  CTATTAGCTGGCGAGTCAACTCACGTTGTTGGAATGACTAGGGACAACTAGACTTCAAGT
  GATCTTTCATCCCAGGAATATAAATAGTTTCTGGAAAGGACACTGACAACTTCAAAGCAA
  AATGAAGTTCTTTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAA
  TACACAACAAGGACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGC
  TCTTGAATGTGAGCGATATTTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACC
  AAATGAAAATGTTGCAATTCACAACCCTTTCAGACCTTGGTGGGAAAGATACCAACCAGT
  TAGCTATAAATTATGCACAAGATCTGGAAATGAAGATGAATTTAGAAACATGGTGACTAG
  ATGTAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATATGTCTGGTAA
  TGCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGA
  CTTTCCAGCAGTCCCATATTCTGGATGGGATTNTAATGATGGTAAATGTAAAACTGGAAG
  TGGAGATATCGAGAACTACAATGATGCTACTCANGTCAGAGATTGTCGTCTGGTTGGTCT
  TCTTGATCTTGCACTGGAGAAAGATATGTGCCGTCCAGATTGCCGATATATGAATCATCT
  CATTGACATTGNTGTTGCAGGGNTCAGACTTGATGCTTCCAACACATGTGGCCTGNAGAC
  ACTAAGGCCATTTGGNACAAACTGCATAATCTAACAGTNACTGTNTCCNTGCCAGAAGTA
  ACCCTTCATTTACCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020978 unedited
  ATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTTTGAGGATA
  TGCATTTAATTTTAAATTTTATAATTTAGATTCAGCATGAATTGCAATAAATGGATCCTC
  AGCAGAGTTACTAATAGAAAAATGAGCTTTGCCATCGTCAGAAACGTAGATTTTAATGCC
  TGTGCAATTGCCATTAATTTTATCTCCAGAAATGACATCACAGTATGTGCCAGCAGGAAG
  ACCAGTTTGCAAAGTTAAAGAAAATGTCCAGTCATCATTGTTGAAAACAATGAATCCTCT
  GTTTCCTCTCCCAAAAGCCACTTGGTTGCTCCCATTATCATACCAGTTTGTAAAAGGCTG
  GCCATCCACTACATTGCGGAAATTAACCATGTTCCTTATTTGGCGCCATCGATGTTCACA
  GACCCAGTCATTGCCACAAGTAGTGTCTGGATTAATAGTAACTTCTTTAATTACTCCATT
  ATTATTTGGTGGCCCAACCCAATCATTAACATCGTTTCCATTTTGAAACTGTCTTGGCCA
  ACGGTAGCTTGACATTACTCGTGTAAAACCATAAGGATGAGCAAGCATAAATCCAACTGC
  CATTTTATACAGCCTAGCATCCCAGAAGGTAAGAATAGAGGCTCCTCCAGCCCCATGTCC
  TCGTTGATTGTCATGGTTATCCACAAAGACAAGTGCTCTGTCAGAAGGCATGAAACCCCA
  ACCTTCTCCCCAGTTCTTTAGGTAAGACATCTCTCTCCATTCCACTTGCGAATAACTGTG
  CCGAGTTNTGACCATACTTGAATCTGTCACCGGNCATTCCAAGTAGTACTGCTTTAATTG
  CTCACACCCAGATCATACCTCTGTAATGAAGGTTACTTCTGCAGGNACAGTCTGTTAGAT
  ATGCAGTGTCCAAATGCCTTTGTCTCAGCCCAGTGCTGGAGCTCAGCTGAACTGCACACA
  TGTATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020978
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020978.3, NP_066188.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 1536 bp
• Locus ID: 280
• Cytogenetics: 1p21.1
• Domains: alpha-amylase, Aamy_C, Aamy
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: 'Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jun 2013]'