TRMT12 (NM_017956) Human Untagged Clone

CAT#: SC113870

TRMT12 (untagged)-Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRMT12
• Synonyms: TRM12; TYW2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113870 sequence for NM_017956 edited (data generated by NextGen Sequencing)
  ATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAGAGAAAGTGGGAAGCCCGTGGCTGTT
  GTCGCAGTTGTGACTGAGCCTTGGTTTACCCAGCGATACAGAGAATATCTCCAGAGGCAG
  AAACTCTTTGATACACAGCACCGTGTGGAAAAGATGCCGGATGGCTCGGTGGCGCTACCG
  GTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCAGGAGCTGAGGAATCGTGTTGCCCCA
  GGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCCTGTTCCTTCGAAGAGGGCCCAGGGT
  TGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAGTCGCTGGGTGGAGGGTCGGGGAGTC
  AAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCGATCATGGCAACGGCATGGTAATCTC
  TTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCAGTGGAAAAATCTGGGACCGGAACTC
  TGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCGTTTGGCAAAACGAGGGCGGGTATCA
  CCGGATGGTACTCGAACTCCAGCAGTGACACTGCTGCTGGGTGACCATGGCTGGGTAGAG
  CATGTGGATAATGGTATCCGTTATAAGTTTGACGTGACCCAGTGTATGTTCTCCTTTGGA
  AACATCACTGAGAAGCTTCGAGTGGCATCGTTGTCCTGTGCTGGAGAAGTGCTGGTGGAT
  CTCTATGCAGGGATTGGTTATTTTACATTGCCTTTCCTAGTTCATGCTGGTGCTGCCTTC
  GTCCATGCTTGTGAGTGGAATCCCCATGCTGTAGTTGCTCTGAGAAATAACCTTGAGATC
  AATGGAGTAGCAGATCGGTGCCAAATACACTTTGGAGATAACAGAAAACTGAAGCTCTCA
  AATATTGCAGATAGGGTGATCCTGGGGCTGATTCCCAGCTCTGAAGAAGGCTGGCCCATT
  GCCTGCCAAGTGTTAAGGCAGGATGCTGGAGGCATTTTGCATATCCACCAAAATGTGGAA
  TCTTTCCCAGGGAAGAATCTTCAGGCTCTTGGAGTCAGCAAAGTAGAGAAAGAGCATTGG
  CTGTATCCTCAGCAAATTACCACCAACCAATGGAAAAATGGAGCTACCAGGGATTCTAGG
  GGAAAAATGCTGTCACCAGCCACCAAGCCAGAGTGGCAAAGGTGGGCAGAATCTGCAGAA
  ACTCGAATCGCCACTCTTCTTCAGCAGGTGCATGGGAAACCATGGAAGACACAAATTCTG
  CACATCCAACCAGTGAAATCCTATGCTCCCCATGTGGATCACATAGTCCTGGATCTGGAA
  TGCTGCCCCTGTCCTTCAGTTGGCTAG
  
  Clone variation with respect to NM_017956.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017956 unedited
  GGGGTCAGATTTGTATACGACTTCTATAGGCGGCCGCGNAATTCGCACGAGGGCCGGGTG
  AGCTGCAGGCTACCTTATTTAAGACCGGGAATTTAGTCAGCCTGGTGAGCCGACTCTGAG
  GAGATGGAGTATCGCTGAGGTGATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAGAGA
  AAGTGGGAAGCCCGTGGCTGTTGTCGCAGTTGTGACTGAGCCTTGGTTTACCCAGCGATA
  CAGAGAATATCTCCAGAGGCAGAAACTCTTTGATACACAGCACCGTGTGGAAAAGATGCC
  GGATGGCTCGGTGGCGCTACCGGTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCAGGA
  GCTGAGGAATCGTGTTGCCCCAGGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCCTGT
  TCCTTCGAAGAGGGCCCAGGGTTGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAGTCG
  CTGGGTGGAGGGTCGGGGAGTCAAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCGATC
  ATGGCAACGGCATGGTAATCTCTTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCAGTG
  GAAAAATCTGGGACCGGAACTCTGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCGTTT
  GGCAAAACGAGGGCGGGTATCACCGGATGGTACTCGAACTCCAGCAGTGACACTGCTGCT
  GGGTGACCATGGCTGGGTAGAGCATGTGGATAATGGTATCCGTTATAAGTTTGACGTGAC
  CCAGTGTATGTTCTCCTTTGGAAACATCACTGAGAAGCTTCGAGTGGCATCGTTGTCCTG
  TGCTNGGAGAGTGCTGGGTGGATCTCTATGCAGGNATTGGTTATTTTACATTGCCTTTNC
  TAGNTCATGCTGTGCTGNCTTCGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017956
• ORF Size: 1347 bp
• Insert Size: 2200
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_017956.1, NP_060426.1
• RefSeq Size: 2061
• RefSeq ORF: 1347
• Locus ID: 55039
• Domains: Met_10
• Gene Summary: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006). [supplied by OMIM, Mar 2008]