SEC31A (NM_014933) Human Untagged Clone

CAT#: SC114771

SEC31A (untagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEC31A
• Synonyms: ABP125; ABP130; HSPC275; HSPC334; SEC31L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114771 sequence for NM_014933 edited (data generated by NextGen Sequencing)
  
  
  ATGAAGTTAAAGGAAGTAGATCGTACAGCCATGCAGGCATGGAGCCCTGCCCAGAATCACCCCATTTACC
  TAGCAACAGGAACATCTGCTCAGCAATTGGATGCAACATTTAGTACGAATGCTTCCCTTGAGATATTTGA
  ATTAGACCTCTCTGATCCATCCTTGGATATGAAATCTTGTGCCACATTCTCCTCTTCTCACAGGTACCAC
  AAGTTGATTTGGGGGCCTTATAAAATGGATTCCAAAGGAGATGTCTCTGGAGTTCTGATTGCAGGTGGTG
  AAAATGGAAATATTATTCTCTATGATCCTTCTAAAATTATAGCTGGAGACAAGGAAGTTGTGATTGCCCA
  GAATGACAAGCATACTGGCCCAGTGAGAGCCTTGGATGTGAACATTTTCCAGACTAATCTGGTAGCTTCT
  GGTGCTAATGAATCTGAAATCTACATATGGGATCTAAATAATTTTGCAACCCCAATGACACCAGGAGCCA
  AAACACAGCCGCCAGAAGATATCAGCTGCATTGCATGGAACAGACAAGTTCAGCATATTTTAGCATCAGC
  CAGTCCCAGTGGCCGGGCCACTGTATGGGATCTTAGGAAAAATGAGCCAATCATCAAAGTCAGTGACCAT
  AGTAACAGAATGCATTGTTCTGGGTTGGCATGGCATCCTGATGTTGCTACTCAGATGGTCCTTGCCTCCG
  AGGATGACCGGTTACCAGTGATCCAGATGTGGGATCTTCGATTTGCTTCCTCTCCACTTCGTGTCCTGGA
  AAACCATGCCAGGGGGATTTTGGCAATTGCTTGGAGCATGGCAGATCCTGAATTGTTACTGAGCTGTGGA
  AAAGATGCTAAGATTCTCTGCTCCAATCCAAACACAGGAGAGGTGTTATATGAACTTCCCACCAACACAC
  AGTGGTGCTTCGATATTCAGTGGTGTCCCCGAAATCCTGCTGTCTTATCAGCTGCTTCGTTTGATGGGCG
  TATCAGTGTTTATTCTATCATGGGAGGAAGCACAGATGGTTTAAGACAGAAACAAGTTGACAAGCTTTCA
  TCATCTTTTGGGAATCTTGATCCCTTTGGCACAGGACAGCCCCTTCCTCCGTTACAAATTCCACAGCAGA
  CTGCTCAGCATAGTATAGTGCTGCCTCTGAAGAAGCCGCCCAAGTGGATTCGAAGGCCTGTTGGTGCTTC
  TTTTTCATTTGGAGGCAAACTGGTTACGTTTGAGAATGTCAGAATGCCTTCTCATCAGGGAGCTGAGCAG
  CAGCAGCAGCAGCACCATGTGTTCATTAGTCAGGTTGTAACAGAAAAGGAGTTCCTCAGCCGATCAGACC
  AACTTCAGCAGGCTGTGCAGTCACAAGGATTTATCAATTATTGCCAAAAAAAAATTGATGCTTCTCAGAC
  TGAATTTGAGAAAAATGTGTGGTCCTTTTTGAAGGTAAACTTTGAGGATGATTCTCGTGGAAAATACCTT
  GAACTTCTAGGATACAGAAAAGAAGATCTAGGAAAGAAGATTGCTTTGGCCTTGAACAAAGTGGATGGAG
  CCAATGTGGCTCTTAAAGACTCTGACCAAGTAGCACAGAGTGATGGGGAGGAGAGCCCTGCTGCTGAAGA
  GCAGCTCTTGGGAGAGCACATTAAAGAGGAAAAAGAAGAATCTGAATTTCTACCCTCATCTGGAGGAACA
  TTTAATATCTCTGTCAGTGGGGACATTGATGGTTTAATTACTCAGGCTTTGCTGACGGGCAATTTTGAGA
  GTGCTGTTGACCTTTGTTTACATGATAACCGCATGGCCGATGCCATTATATTGGCCATAGCAGGTGGACA
  AGAACTCTTGGCTCGAACCCAGAAAAAATACTTCGCAAAATCCCAAAGCAAAATTACCAGGCTCATCACT
  GCAGTGGTGATGAAGAACTGGAAAGAGATTGTTGAGTCTTGTGATCTTAAAAATTGGAGAGAGGCTTTAG
  CTGCAGTATTGACTTATGCAAAGCCGGATGAATTTTCAGCCCTTTGTGATCTTTTGGGAACCAGGCTTGA
  AAATGAAGGAGATAGCCTCCTGCAGACTCAAGCATGTCTCTGCTATATTTGTGCAGGGAATGTAGAGAAA
  TTAGTTGCATGTTGGACTAAAGCTCAAGATGGAAGCCACCCTTTGTCACTTCAGGATCTGATTGAGAAAG
  TTGTCATCCTGCGAAAAGCTGTGCAACTCACTCAAGCCATGGACACTAGTACTGTAGGAGTTCTCTTGGC
  TGCGAAGATGAGTCAGTATGCCAATTTGTTGGCAGCTCAGGGCAGTATTGCTGCAGCCTTGGCTTTTCTT
  CCTGACAACACCAACCAGCCAAATATCATGCAGCTTCGTGACAGACTTTGTAGAGCACAAGGAGAGCCTG
  TAGCAGGACATGAATCACCTAAAATTCCGTACGAGAAACAGCAGCTCCCCAAGGGCAGGCCTGGACCAGT
  TGCTGGCCACCACCAGATGCCAAGAGTTCAAACTCAACAATATTATCCCCATGGAGAAAATCCTCCACCT
  CCGGGTTTCATAATGCATGGAAATGTTAATCCAAATGCTGCTGGTCAGCTTCCCACATCTCCAGGTCATA
  TGCACACCCAGGTACCACCTTATCCACAGCCACAGCCTTATCAACCAGCCCAGCCGTATCCCTTCGGAAC
  AGGGGGGTCAGCAATGTATCGACCTCAGCAGCCTGTTGCTCCTCCTACTTCAAACGCTTACCCTAACACC
  CCTTACATATCTTCTGCTTCTTCCTATACTGGGCAGTCTCAGCTGTACGCAGCACAGCACCAGGCCTCTT
  CACCTACCTCCAGCCCTGCTACTTCTTTCCCTCCTCCCCCTTCCTCTGGAGCATCCTTCCAGCATGGCGG
  ACCAGGAGCTCCACCATCATCTTCAGCTTATGCACTGCCTCCTGGAACAACAGGTACACTGCCTGCTGCC
  AGTGAGCTGCCTGCGTCCCAAAGAACAGGTCCTCAGAATGGTTGGAATGACCCTCCAGCTTTGAACAGAG
  TACCCAAAAAGAAGAAGATGCCTGAAAACTTCATGCCTCCTGTTCCCATCACATCACCAATCATGAACCC
  GTTGGGTGACCCCCAGTCACAAATGCTGCAGCAACAGCCTTCAGCTCCAGTACCACTGTCAAGCCAGTCT
  TCATTCCCACAGCCACATCTTCCAGGTGGCCAGCCCTTCCATGGCGTACAGCAACCTCTTGGTCAAACAG
  GCATGCCACCATCTTTTTCAAAGCCCAATATTGAAGGTGCCCCAGGGGCTCCTATTGGAAATACCTTCCA
  GCATGTGCAGTCTTTGCCAACAAAAAAAATTACCAAGAAACCTATTCCAGATGAGCACCTCATTCTAAAG
  ACCACATTTGAGGATCTTATTCAGCGCTGCCTTTCTTCAGCAACAGACCCTCAAACCAAGAGGAAGCTAG
  ATGATGCCAGCAAACGTTTGGAGTTTCTGTATGATAAACTTAGGGAACAGACACTTTCACCAACAATCAC
  CAGTGGTTTACACAACATTGCAAGGAGCATTGAAACTCGAAACTACTCAGAAGGATTGACCATGCATACC
  CACATAGTTAGCACCAGCAACTTCAGTGAGACCTCTGCTTTCATGCCAGTTCTCAAAGTTGTTCTCACCC
  AGGCCAATAAGCTGGGTGTCTAA
  
  Clone variation with respect to NM_014933.3
  597:g=>a
• Restriction Sites: NotI-NotI
• ACCN: NM_014933
• ORF Size: 3663 bp
• Insert Size: 4200
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014933.2, NP_055748.2
• RefSeq Size: 4273
• RefSeq ORF: 3663
• Locus ID: 22872
• Domains: WD40
• Gene Summary: The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) uses an alternate splice site in the 5' UTR, resulting in lacking of 3 nts, compared to variant 5. It encodes the same protein as variant 5 (isoform 1).