ASPA (NM_000049) Human Untagged Clone

CAT#: SC114775

ASPA (untagged)-Human aspartoacylase (ASPA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASPA
• Synonyms: ACY2; ASP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114775 sequence for NM_000049 edited (data generated by NextGen Sequencing)
  ATGACTTCTTGTCACATTGCTGAAGAACATATACAAAAGGTTGCTATCTTTGGAGGAACC
  CATGGGAATGAGCTAACCGGAGTATTTCTGGTTAAGCATTGGCTAGAGAATGGCGCTGAG
  ATTCAGAGAACAGGGCTGGAGGTAAAACCATTTATTACTAACCCCAGAGCAGTGAAGAAG
  TGTACCAGATATATTGACTGTGACCTGAATCGCATTTTTGACCTTGAAAATCTTGGCAAA
  AAAATGTCAGAAGATTTGCCATATGAAGTGAGAAGGGCTCAAGAAATAAATCATTTATTT
  GGTCCAAAAGACAGTGAAGATTCCTATGACATTATTTTTGACCTTCACAACACCACCTCT
  AACATGGGGTGCACTCTTATTCTTGAGGATTCCAGGAATAACTTTTTAATTCAGATGTTT
  CATTACATTAAGACTTCTCTGGCTCCACTACCCTGCTACGTTTATCTGATTGAGCATCCT
  TCCCTCAAATATGCGACCACTCGTTCCATAGCCAAGTATCCTGTGGGTATAGAAGTTGGT
  CCTCAGCCTCAAGGGGTTCTGAGAGCTGATATCTTGGATCAAATGAGAAAAATGATTAAA
  CATGCTCTTGATTTTATACATCATTTCAATGAAGGAAAAGAATTTCCTCCCTGCGCCATT
  GAGGTCTATAAAATTATAGAGAAAGTTGATTACCCCCGGGATGAAAATGGAGAAATTGCT
  GCTATCATCCATCCTAATCTGCNGGATCAAGACTGGAAACCACTGCATCCTGGGGATCCC
  ATGTTTTTAACTCTTGATGGGAAGACGATCCCACTGGGCGGAGACTGTACCGTGTACCCC
  GTGTTTGTGAATGAGGCCGCATATTACGAAAAGAAAGAAGCTTTTGCAAAGACAACTAAA
  CTAACGCTCAATGCAAAAAGTATTCGCTGCTGTTTACATTAG
  
  Clone variation with respect to NM_000049.2
  743 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000049 unedited
  TGGATTTTGTAACACGATTTTCTATAGGGCGGCCGCGAATTCGGCACGAGGATGACTTCT
  TGTCACATTGCTNGAAGAACATATACAAAAGGTTGCTATCTTTGGAGGAACCCATGGGAA
  TGAGCTAACCGGAGTATTTCTGGTTAAGCATTGGCTAGAGAATGGCGCTGAGATTCAGAG
  AACAGGGCTGGAGGTAAAACCATTTATTACTATTTTTATNACAGCGAAGAAGTGTACCAG
  ATATATTGACTGTGACCTGAATCGCATTTTTGACCTTGAAAATCTTGGCAAAAAAATGTC
  AGAAGATTTGCCATATGAAGTGAGAAGGGCTCAAGAAATAAATCATTTATTTGGTCCAAA
  AGACAGTGAAGATTCCTATGACATTATTTTTGACCTTCACAACACCACCTCTAACATGGG
  GTGCACTCTTATTCTTGAGGATTCCAGGAATAACTTTTTAATTCAGATGTTTCATTACAT
  TAAGACTTCTCTGGCTCCACTACCCTGCTACGTTTATCTGATTGAGCATCCTTCCCTCAA
  ATATGCGACCACTCGTTCCATAGCCAAGCATCCTGTGGGCATAGAAGTTGGCCCTCACCC
  TCAAGGCGTTCTGAGAGCTGATATCTTGGATCACATGACAAAAAGATTAAACATGCCTTT
  GATTTTATACATTCTTTCAATGAAGGAAAAGAATTTCCTCCCTGCGCCATTGGAGTCCTA
  TAAAATTATAGAGAAAGGTTGATTACCCTCGGGATGAAAACTGTAGCAACTCGGTGCTTA
  TCATTCCCTCCTAATCTGCAGAGGCATAAGAACTGGAACCCACTGCATCCTGCGGATCCC
  ATGTCTTTAAACTTTGGATCGGAAAACGATTCCCCTGTGGCGGAGACTGTCCCGTGGCCC
  CCGGGCTTGTGACGGACGCCCGCTATTACTAAAGCAGGAATCTTCTTGCAAGCACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000049 unedited
  TACCGCGGGCCGCACTTCTAGAAAGTCGAAGTTTTTTTCTTTTTTTTTCTTTTCTTTTTC
  CACAGAATCACCATAGCAGATATAATAAATAAATCATAAAAAACCTTGAAATATTATCAA
  AGGGTGACACAGAGACAAGGAGTGAGCACATGCTGTTGGAAACATGGCACTGTTAAACTT
  TCTAAACACAGGGTTGCCAAAAACCTTCAATTTGGAAAAAAAGTACAGTATCCGTGAAGT
  GTATTAAGGCAAAGCACAATAAAACAAATAACTTTATATATATCTATTTCAATTTTATAT
  TCTGAATACAAAGGCTGTTTATTAAAAATGTTGAGCTACCCAAGTATCATGTATAATAAA
  CTATATAGAAATAGGAAACACTTCTTTGAATAAGCTACATACATAAAATATGATATCTTT
  AAAGATATATTAATTAATTTAAAAAATTTGCTTAAATGCCTACCGAATAAGGCACTGTGC
  TAGGAGCTATGTATGCAGTTGAATAAGGCACAACCCTACTCTTAAGGAGCTTACAGACTA
  GCAGAATTTGTAAGACACCGTGTAAGATGTAAGCTGGAAGTGATTTCTAATGTAAACAGC
  AGCGAATACTTTCTGCATTGAGCGTTAGTTTAGTTGCCTTTGCAAAAGCTTCTTTCTTTT
  CGTATATGCGGCCTCATTCACAAACACGGGTACACGGTACAGTCTCCGCCCAGGGGGATC
  GTCTTCCATCAAGAGTTAAAACATGGGATCCCCAGTATCAGTGGTTTCCAGTCTTGATCC
  TCTGAACATAAGATGGATGATAGCACCTTTTTTCCATCTCATTCCGGGGAAATCAACTTT
  CTCTTTAATTTATAAACCCCAATGCGCAAGGAGGAATTCTTTTCCTCCTGAAAGAAGTTA
  AAATCAGAACTGTTTATCATCTTCTCATTGACCCAAATTACCTTTAAAACCCTGAGGCGA
  GACAATTCTTTCCCAGAACTGGTTTGGAAAAAGTCCCTTTTGAGGAGGAGCCAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000049
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000049.2, NP_000040.1
• RefSeq Size: 1435 bp
• RefSeq ORF: 942 bp
• Locus ID: 443
• Cytogenetics: 17p13.2
• Domains: Aste_AspA
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Histidine metabolism
• Gene Summary: 'This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) and variant 2 encode the same protein.