TRAM1 (NM_014294) Human Untagged Clone

CAT#: SC115096

TRAM1 (untagged)-Human translocation associated membrane protein 1 (TRAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAM1
• Synonyms: PNAS8; TRAM; TRAMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115096 sequence for NM_014294 edited (data generated by NextGen Sequencing)
  ATGGCGATTCGCAAGAAAAGCACCAAGAGCCCCCCAGTGCTGAGCCACGAATTCGTCCTG
  CAGAATCACGCGGACATCGTCTCCTGTGTGGCGATGGTCTTCCTGCTGGGGCTCATGTTT
  GAGATAACGGCAAAAGCTTCTATCATTTTTGTTACTCTTCAGTACAATGTCACCCTCCCA
  GCAACAGAAGAACAAGCTACTGAATCAGTGTCCCTTTATTACTATGGCATCAAAGATTTG
  GCTACTGTTTTCTTCTACATGCTAGTGGCGATAATTATTCATGCCGTAATTCAAGAGTAT
  ATGTTGGATAAAATTAACAGGCGAATGCACTTCTCCAAAACAAAACACAGCAAGTTTAAT
  GAATCTGGTCAGCTTAGTGCGTTCTACCTTTTTGCCTGTGTTTGGGGCACATTCATTCTC
  ATCTCTGAAAACTACATCTCAGACCCAACTATCTTATGGAGGGCTTATCCCCATAACCTG
  ATGACATTTCAAATGAAGTTTTTCTACATATCACAGCTGGCTTACTGGCTTCATGCTTTT
  CCTGAACTCTACTTCCAGAAAACCAAAAAAGAAGATATTCCTCGTCAGCTTGTCTACATT
  GGTCTTTACCTCTTCCACATTGCTGGAGCTTACCTTTTGAACTTGAATCATCTAGGACTT
  GTTCTTCTGGTGCTACATTATTTTGTTGAATTTCTTTTCCACATTTCCCGCCTGTTTTAT
  TTTAGCAATGAAAAGTATCAGAAAGGATTTTCTCTGTGGGCAGTTCTTTTTGTTTTGGGA
  AGACTTCTGACTTTAATTCTTTCAGTACTGACTGTTGGTTTTGGCCTTGCAAGAGCAGAA
  AATCAGAAGCTGGATTTCAGTACTGGAAACTTCAATGTGTTAGCTGTTAGAATCGCTGTT
  CTGGCATCCATTTGCGTTACTCAGGCATTTATGATGTGGAAGTTCATTAATTTTCAGCTT
  CGAAGGTGGAGGGAACATTCTGCTTTTCAGGCACCAGCTGTGAAGAAGAAACCAACAGTA
  ACTAAAGGCAGATCTTCTAAAAAAGGAACAGAAAATGGTGTGAATGGAACATTAACTTCA
  AATGTAGCAGACTCTCCCCGGAATAAAAAAGAGAAATCTTCATAA
  
  Clone variation with respect to NM_014294.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014294 unedited
  GTGCGCATTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGTTTTTTTTCC
  CCCGGGCGGCCCGGCGGCTGCGTACTGGCTGTGGGATGGGAAGTGAAGCCCCAGCGAGCG
  GCTGCAGCGGGGCCGTGAGGAGCAGCCAGCGGGAGGCGGCGGCGAGTCGGTGAGCAGCTG
  GGAAGAGCAGAACCGGGGCGGAGCACCTGCAGGCGCGGGCGGCGGCCCCACCATGGCGAT
  TCGCAAGAAAAGCACCAAGAGCCCCCCAGTGCTGAGCCACGAATTCGTCCTGCAGAATCA
  CGCGGACATCGTCTCCTGTGTGGCGATGGTCTTCCTGCTGGGGCTCATGTTTGAGATAAC
  GGCAAAAGCTTCTATCATTTTTGTTACTCTTCAGTACAATGTCACCCTCCCAGCAACAGA
  AGAACAAGCTACTGAATCAGTGTCCCTTTATTACTATGGCATCAAAGATTTGGCTACTGT
  TTTCTTCTACATGCTAGTGGCGATAATTATTCATGCCGTAATTCAAGAGTATATGTTGGA
  TAAAATTAACAGGCGAATGCACTTCTCCAAAACAAAACACAGCAAGTTTAATGAATCTGG
  TCAGCTTAGTGCGTTCTACCTTTTTGCCTGTGTTTGGGGCACATTCATTCTCATCTCTNG
  AAACTACATCTCAGACCCAACTATCTTATGAAGGGCTTATCCCCATAACCTGATGACATT
  TAAATGAAGTTTTTCTACATATCACAGCTGGGTTACTGGCTTCATGCTTTTCCTGAACTC
  TACTTNCAAAAAACCAAAAAAGAAGATATTCCTCGTCAGCTTGGCTACATTGGTCTTTAC
  CTCTTNCCCATTGTTGGGCCTACCTTTTTGACTTGAATCATTTAGGACTTGGTTCTTTGG
  GGCTCCATTATTTTGTTGAATTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014294 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTAAAAATTTTAT
  TTTGATTGATTTCTCAATGTATAGTTCAGTATAATGCCAGTTTTTAATGGCAAAAATTTG
  GTTCCACTGAAACTCCATAATGCTACAGAGAGCTACTACTTTTTCCAGGAAGTAGGTTAA
  CAGCTAGAAAGAAAAAGGACAATTTCCTAGCAGCATGGCAACTTAAACTGCAGATCTAAT
  AGGTCTGCAACTTTTACACTAAAAATGGCACAAACAGCTGGTGACACAAGTGAGAAATGG
  GGAACAAGATGTGAACACTGAAAAGAACAATATATATACTGTAAATATGATGAATAAACC
  AAATGTAGCTATAAGAATCTTAAAGGATGATTATAGAAAAGGGAACCAAATATTTTCCTC
  AAATGCTTTTTAGAGCCTTTCTGTAGCGATACAAATATATATATATATATTTATCCAAAA
  ATATGTTTTATACAGATAAATGTTTCTCAATTAAAGATGGGACCAAAGCTATAGGTATAA
  CATAAAGCACATTGTCTTTTGTAAGACTATTTATCCACCTGAATTTTCAATTTCTTTAAA
  GTTAGTGCATTAAATGTTTTCCAAATATAATTACCTGTCAGTCTTTNTATAGATATAAAT
  CAAGTAGGCATTATGTTTTAAAAGTGTTTGCAGGTTAAACTTTTCTAGATGCTGTGCATA
  TTAAACTTATTATGAGCCCCATTNAGAATCATTATTAATAAACATATCAAAAAGGGCTAT
  ATGTCAGATTTTCCTGTTCATAGTAAATATTTTCTGATTTCCAGTTTACAAGTATTGAAA
  ATGCACAAGAAAGAATTTACTTCACAAGTACTTNTAAGAATATACTTTGATTAATATGTA
  TGGTAGNAAAACTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014294
• ORF Size: 1125 bp
• Insert Size: 2740
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_014294.3, NP_055109.1
• RefSeq Size: 2808
• RefSeq ORF: 1125
• Locus ID: 23471
• Domains: LAG1, TLC
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.