FOXI1 (NM_012188) Human Untagged Clone

CAT#: SC115475

FOXI1 (untagged)-Human forkhead box I1 (FOXI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXI1
• Synonyms: FKH10; FKHL10; FREAC-6; FREAC6; HFH-3; HFH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115475 sequence for NM_012188 edited (data generated by NextGen Sequencing)
  ATGAGCTCCTTCGACCTGCCGGCGCCCTCCCCACCTCGCTGCAGCCCCCAGTTCCCCAGC
  ATCGGCCAGGAGCCCCCCGAGATGAACCTCTACTATGAGAACTTCTTCCACCCACAGGGC
  GTGCCCAGCCCTCAGCGGCCCTCCTTCGAGGGGGGCGGCGAGTATGGGGCCACCCCCAAC
  CCCTACCTCTGGTTCAACGGGCCCACCATGACCCCGCCACCCTACCTGCCCGGCCCCAAC
  GCCAGCCCCTTCCTGCCCCAGGCCTATGGAGTGCAGAGACCGCTGCTGCCCAGCGTGTCG
  GGGCTTGGGGGGAGCGACCTGGGCTGGCTGCCCATCCCCTCGCAGGAGGAGCTGATGAAG
  CTGGTGCGGCCACCCTATTCCTACTCGGCTCTCATCGCCATGGCCATCCACGGGGCACCC
  GACAAGCGCCTCACTCTCAGCCAGATCTACCAGTACGTGGCCGACAACTTCCCCTTCTAC
  AACAAGAGCAAGGCCGGCTGGCAGAACTCCATCCGCCACAACCTGTCGCTCAACGACTGC
  TTCAAGAAGGTGCCCCGCGACGAGGACGACCCGGGCAAAGGGAATTACTGGACCCTGGAC
  CCCAACTGTGAGAAAATGTTCGACAATGGAAATTTCCGCAGGAAAAGGAAGAGAAAATCA
  GATGTTTCCTCTAGCACAGCCTCCTTGGCCTTAGAGAAGACAGAGAGCAGTCTCCCGGTG
  GACAGCCCCAAGACCACGGAGCCTCAGGACATCTTGGATGGAGCCTCACCAGGGGGCACC
  ACCAGCTCCCCAGAGAAGCGGCCCTCCCCTCCCCCATCAGGCGCCCCTTGCCTTAACAGC
  TTCCTTTCCTCTATGACAGCCTATGTGAGCGGGGGGAGCCCCACGAGCCACCCCTTGGTC
  ACACCAGGACTGAGCCCTGAGCCCAGTGACAAGACGGGGCAGAACTCACTGACCTTCAAC
  TCCTTCTCCCCGCTCACCAACCTCAGCAACCACAGCGGTGGGGGTGACTGGGCGAACCCC
  ATGCCCACCAACATGCTCAGCTACGGAGGATCTGTGCTCAGCCAATTCAGCCCTCACTTC
  TACAACAGTGTCAACACCAGTGGTGTCCTCTACCCCAGGGAGGGCACCGAGGTCTAG
  
  Clone variation with respect to NM_012188.4
  279 g=>a;1044 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012188 unedited
  CACAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCCGGGGTGC
  AGGTGCCAGGCAGGTGGCTCCGGCCAGCCCAGCCCCAGCATGAGCTCCTTCGACCTGCCG
  GCGCCCTCCCCACCTCGCTGCAGCCCCCAGTTCCCCAGCATCGGCCAGGAGCCCCCCGAG
  ATGAACCTCTACTATGAGAACTTCTTCCACCCACAGGGCGTGCCCAGCCCTCAGCGGCCC
  TCCTTCGAGGGGGGCGGCGAGTATGGGGCCACCCCCAACCCCTACCTCTGGTTCAACGGG
  CCCACCATGACCCCGCCACCCTACCTGCCCGGCCCCAACGCCAGCCCCTTCCTGCCCCAG
  GCCTATGGAGTGCAGAGACCGCTGCTGCCCAGCGTGTCGGGGCTTGGGGGGAGCGACCTG
  GGCTGGCTGCCCATCCCCTCGCAGGAGGAGCTGATGAAGCTGGTGCGGCCACCCTATTCC
  TACTCGGCTCTCATCGCCATGGCCATCCACGGGGCACCCGACAAGCGCCTCACTCTCAGC
  CAGATCTACCAGTACGTGGCCGACAACTTCCCCTTCTACAACAAGAGCAAGGCCGGCTGG
  CAGAACTCCATCCGCCACAACCTGTCGCTCAACGACTGCTTCAAGAAGGTGCCCCGCGAC
  GAGGACGACCCGGGCAAAGGGAATTACTGGACCCTGGNACCCAACTGTGAGAACATGGGT
  CGACATGTGAATTTCCGCAGGAAAGGGAAGAGAAATCAGATGTTTCCTCTAGCACAGCCT
  TCTTGGCCTTAGAGAGACAGAGAGCAGTCTCCCGGTGGACAGGCCCNAGACCACGAAGCC
  TCCAGACATCTTGGATGGAGCCTCACCAGGGGGCACCANCAGGCTCCCAGAGAAGCCGGC
  CTCCCCTCCCCCATCAGGCGCCCCTTGGCCTAACAGACTTCCTTTCCTCTATGACAGGCT
  TGTNGAGCGGGGGAGGCCCACGGANCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012188 unedited
  AGNANGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGTTCTGTTCAGGAAACAGCT
  ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG
  GTTGTTTGTTTTTTAATTAAAAAGAAAATAAGAAAACAAACAACACTTCCCAGGTACTCA
  CAAAAACAAGGAGAAATCCCAAGAGATCACACAGAGACCCCCTGTGAGGGTGGGCCCAAG
  GCCCCTCCCTTGAGGCCATTGTTTTCTCAAACAAACATCCACCTCTCCTGGGCTAAAAAA
  AAAGGAAGCGCTTATCCAACCTTGTTTCTCTTGGAAGATTCAGCCATCTCCAGCTCACTG
  GTCCATGACATGCACACAGGAAACTGGGCTTTACAAGATTTTACAGATGGGCAGTGCTGG
  GGTGGGAGGATTTAGGGGTACACACCACTCCTCTCACTGATCCCTGGATCTGCTCAAGGT
  TTCTGGAGGGCTGGGGAGCAATGCTGTCTCTCCATGGGGATGCATCGCACATGTCAGATG
  CATTGTTCTGCAGGGCCACCAGGGCTGAGTTTCCTGCACTAAGGCCACTGGTGACTTCCT
  TCCTTGAGTGGTAGCAAAAGGAGTGTCTGCAGGTAAGTTGGGGCAAGCAGAATAAGGACT
  TGGACCAGGACAAGTTGTGCCTAGGCTGGCACTAAATGCTGGTGGACAGAAGGCAGTGGA
  AAGATTTCCCTATATTTGCCATAACAAGCTTTTCACACTGGACCTTCCTTGGCTACTCAA
  CCAATTCTCCATCCTGGCCTTCCATCCTCTGAGGATCACATAAACCCTGGATTGAACTCT
  TAACTCTGCCCTTTACTAGTTTAAATAACCCTGGAAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012188
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012188.3, NP_036320.2
• RefSeq Size: 2294 bp
• RefSeq ORF: 1137 bp
• Locus ID: 2299
• Cytogenetics: 5q35.1
• Domains: FH
• Protein Families: Transcription Factors
• Gene Summary: 'This gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene may play an important role in the development of the cochlea and vestibulum, as well as in embryogenesis. The encoded protein has been found to be required for the transcription of four subunits of a proton pump found in the inner ear, the kidney, and the epididymis. Mutations in this gene have been associated with deafness, autosomal recessive 4. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).