NMDAR1 (GRIN1) (NM_007327) Human Untagged Clone

CAT#: SC115601

GRIN1 (untagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 1 (GRIN1), transcript variant NR1-3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIN1
• Synonyms: GluN1; MRD8; NDHMSD; NDHMSR; NMD-R1; NMDA1; NMDAR1; NR1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115601 sequence for NM_007327 edited (data generated by NextGen Sequencing)
  ATGAGCACCATGCGCCTGCTGACGCTCGCCCTGCTGTTCTCCTGCTCCGTCGCCCGTGCC
  GCGTGCGACCCCAAGATCGTCAACATTGGCGCGGTGCTGAGCACGCGGAAGCACGAGCAG
  ATGTTCCGCGAGGCCGTGAACCAGGCCAACAAGCGGCACGGCTCCTGGAAGATTCAGCTC
  AATGCCACCTCCGTCACGCACAAGCCCAACGCCATCCAGATGGCTCTGTCGGTGTGCGAG
  GACCTCATCTCCAGCCAGGTCTACGCCATCCTAGTTAGCCATCCACCTACCCCCAACGAC
  CACTTCACTCCCACCCCTGTCTCCTACACAGCCGGCTTCTACCGCATACCCGTGCTGGGG
  CTGACCACCCGCATGTCCATCTACTCGGACAAGAGCATCCACCTGAGCTTCCTGCGCACC
  GTGCCGCCCTACTCCCACCAGTCCAGCGTGTGGTTTGAGATGATGCGTGTCTACAGCTGG
  AACCACATCATCCTGCTGGTCAGCGACGACCACGAGGGCCGGGCGGCTCAGAAACGCCTG
  GAGACGCTGCTGGAGGAGCGTGAGTCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGG
  ACCAAGAACGTGACGGCCCTGCTGATGGAGGCGAAAGAGCTGGAGGCCCGGGTCATCATC
  CTTTCTGCCAGCGAGGACGATGCTGCCACTGTATACCGCGCAGCCGCGATGCTGAACATG
  ACGGGCTCCGGGTACGTGTGGCTGGTCGGCGAGCGCGAGATCTCGGGGAACGCCCTGCGC
  TACGCCCCAGACGGCATCCTCGGGCTGCAGCTCATCAACGGCAAGAACGAGTCGGCCCAC
  ATCAGCGACGCCGTGGGCGTGGTGGCCCAGGCCGTGCACGAGCTCCTCGAGAAGGAGAAC
  ATCACCGACCCGCCGCGGGGCTGCGTGGGCAACACCAACATCTGGAAGACCGNNNNNNNN
  NNNNAGAGAGTGCTGATGTCTTCCAAGTATGCGGATGGGGTGACTGGTCGCGTGGAGTTC
  AATGAGGATGGGGACCGGAAGTTCGCCAACTACAGCATCATGAACCTGCAGAACCGCAAG
  CTGGTGCAAGTGGGCATCTACAATGGCACCCACGTCATCCCTAATGACAGGAAGATCATC
  TGGCCAGGCGGAGAGACAGAGAAGCCTCGAGGGTACCAGATGTCCACCAGACTGAAGATT
  GTGACGATCCACCAGGAGCCCTTCGTGTACGTCAAGCCCACGCTGAGTGATGGGACATGC
  AAGGAGGAGTTCACAGTCAACGGCGACCCAGTCAAGAAGGTGATCTGCACCGGGCCCAAC
  GACACGTCGCCGGGCAGCCCCCGCCACACGGTGCCTCAGTGTTGCTACGGCTTTTGCATC
  GACCTGCTCATCAAGCTGGCACGGACCATGAACTTCACCTACGAGGTGCACCTGGTGGCA
  GATGGCAAGTTCGGCACACAGGAGCGGGTGAACAACAGCAACAAGAAGGAGTGGAATGGG
  ATGATGGGCGAGCTGCTCAGCGGGCAGGCAGACATGATCGTGGCGCCGCTAACCATAAAC
  AACGAGCGCGCGCAGTACATCGAGTTTTCCAAGCCCTTCAAGTACCAGGGCCTGACTATT
  CTGGTCAAGAAGGAGATTCCCCGGAGCACGCTGGACTCGTTCATGCAGCCGTTCCAGAGC
  ACACTGTGGCTGCTGGTGGGGCTGTCGGTGCACGTGGTGGCCGTGATGCTGTACCTGCTG
  GACCGCTTCAGCCCCTTCGGCCGGTTCAAGGTGAACAGCGAGGAGGAGGAGGAGGACGCA
  CTGACCCTGTCCTCGGCCATGTGGTTCTCCTGGGGCGTCCTGCTCAACTCCGGCATCGGG
  GAAGGCGCCCCCAGAAGCTTCTCAGCGCGCATCCTGGGCATGGTGTGGGCCGGCTTTGCC
  ATGATCATCGTGGCCTCCTACACCGCCAACCTGGCGGCCTTCCTGGTGCTGGACCGGCCG
  GAGGAGCGCATCACGGGCATCAACGACCCTCGGCTGAGGAACCCCTCGGACAAGTTTATC
  TACGCCACGGTGAAGCAGAGCTCCGTGGATATCTACTTCCGGCGCCAGGTGGAGCTGAGC
  ACCATGTACCGGCATATGGAGAAGCACAACTACGAGAGTGCGGCGGAGGCCATCCAGGCC
  GTGAGAGACAACAAGCTGCATGCCTTCATCTGGGACTCGGCGGTGCTGGAGTTCGAGGCC
  TCGCAGAAGTGCGACCTGGTGACGACTGGAGAGCTGTTTTTCCGCTCGGGCTTCGGCATA
  GGCATGCGCAAAGACAGCCCCTGGAAGCAGAACGTCTCCCTGTCCATCCTCAAGTCCCAC
  GAGAATGGCTTCATGGAAGACCTGGACAAGACGTGGGTTCGGTATCAGGAATGTGACTCG
  CGCAGCAACGCCCCTGCGACCCTTACTTTTGAGAACATGGCCGGGGTCTTCATGCTGGTA
  GCTGGGGGCATCGTGGCCGGGATCTTCCTGATTTTCATCGAGATTGCCTACAAGCGGCAC
  AAGGATGCTCGCCGGAAGCAGATGCAGCTGGCCTTTGCCGCCGTTAACGTGTGGCGGAAG
  AACCTGCAGGATAGAAAGAGTGGTAGAGCAGAGCCTGACCCTAAAAAGAAAGCCACATTT
  AGGGCTATCACCTCCACCCTGGCTTCCAGCTTCAAGAGGCGTAGGTCCTCCAAAGACACG
  AGCACCGGGGGTGGACGCGGCGCTTTGCAAAACCAAAAAGACACAGTGCTGCCGCGACGC
  GCTATTGAGAGGGAGGAGGGCCAGCTGCAGCTGTGTTCCCGTCATAGGGAGAGCTGA
  
  Clone variation with respect to NM_007327.3
  953 g=>n;954 g=>n;955 c=>n;956 c=>n;957 g=>n;958 c=>n;959 t=>n;960 c=>n;961 t=>n;962 t=>n;963 c=>n;964 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007327 unedited
  AAAAGGTACCGGCGCCCGTTCCCTTAAAGGGCGGTAGGCGTGCACGGTGGGAGGTCTATA
  TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG
  CCGCGAATTCGGCACGAGGACGGCCCGGAAGCCCCGCGGGGGATGCGCCGAGGGCCCCGC
  GTTCGCGCCGCGCAGAGCCAGGCCCGCGGCCTGATCCCATGAGCACCATGCGCCTGCTGA
  CGCTCGCCCTGCTGTTCTCCTGCTCCGTCGCCCGTGCCGCGTGCGACCCCAAGATCGTCA
  ACATTGGCGCGGTGCTGAGCACGCGGAAGCACGAGCAGATGTTCCGCGAGGCCGTGAACC
  AGGCCAACAAGCGGCACGGCTCCTGGAAGATTCAGCTCAATGCCACCTCCGTCACGCACA
  AGCCCAACGCCATCCAGATGGCTCTGTCGGTGTGCGAGGACCTCATCTCCAGCCAGGTCT
  ACGCCATCCTAGTTAGCCATCCACCTACCCCCAACGACCACTTCACTCCCACCCCTGTCT
  CCTACACAGCCGGCTTCTACCGCATACCCGTGCTGGGGCTGACCACCCGCATGTCCATCT
  ACTCGGACAAGAGCATCCACCTGAGCTTCCTGCGCACCGTGCCGCCCTACTCCCACCAGT
  CCAGCGTGTGGTTTGAGATGATGCGTGTCTACAGCTGGAACCACATCATCCTGCTGGTCA
  GCGACGACCACGAGGGCCGGGCGGCTCAGAAACGCCTGGAGACGCTGCTGGAGGAGCGTG
  AGTCCCAAGCAGAGAAAGTGCTGCAGTTTGACCCAGGGACCAAAACGTGNACGCCCTGCT
  GATGGAGCGAAAGAGCTGGNAGCCGGGTCATCATCCTTTCTGCCAGCGAGGCGATGCTGC
  CN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007327 unedited
  NCTACTATGTACCGTGGCCGCGCTCNAAGATCGGTTTTTTTTTTTTTTTTTTGCGTGACA
  TTCCTTTAGGCATCACCACTGCATACACCGGGAGGTGGGGGTTGGGCGGTGCGCGCCTGC
  ACCCTGGGGACGGAGGGGCAGAGCGCGGCTGCGCACGGGCCGCACGGGGAAGGCACGCGC
  TCGGGCCCTGGGAGTGCTGGTTCTGTACAAGGTGGCGGGCCGGTCACGCACCCGGATCGA
  GGGCGGGGGCGGGCGGGTGGCGCTCTCCCGTGCCCAAGGTGCAGGGCAGGGTTGAAGACA
  GTCCCGAGCCAGCGTCCGGGGACCCCCGGGGAGGCCCGTTCTGGGCTGCAGGCCGGACGA
  GGAGAGGAGGGGCTCAGCCCTCTCGAGTCTGGAGGATGCGGCCCGAGGCGGCGGGAGGCG
  CTGCCAGCTGGGAAGTCAGGGACGGCCGTGGGGGATGGCAGGGCCAGCCCCGGCGTGTGG
  GGTCAAGGGGCATACGGGGTGTGGGTGGGCGGTGCCGCAAGAAGAGGGGAGGGTGGAGCT
  CAAGCTGCAGAGGAGCGCATGCGCCCGATGAGGGGCGACCCAGCCAGCTGGTGCCATGGG
  CCCCCACTGTGGCTCATGCTGGTGCTGCGGGAATGATGGGGGCTGCCGTCTCTGCTTCCA
  GCCCCTTCGGCTGGGGCCAGTTAGCCCCCGCTCCCCACTGCTTATAGACCATAAGGCCCT
  GCCTCTGGCGGGAGCGCCCTGGGGCCTTGGCCCAGCCGACTCAACTTTGGCCCGTTCTTA
  ACCGTTTCGGGTCGGACCTTGGTTCCACAAGGGCTGCCCGCCCGGCATCGGGTGGGCCGG
  GCCGGCGGACGGGAGGCCCAGGTGGGCAGTCTTCTGGGGCGGTGGGGCTTCCCCAGGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007327
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007327.1, NP_015566.1
• RefSeq Size: 5137 bp
• RefSeq ORF: 2817 bp
• Locus ID: 2902
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Huntington's disease, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (GluN1-1a, also known as NR1-3) represents the longest transcript and encodes protein isoform GluN1-1a (also known as isoform NR1-3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.