PEAMT (PEMT) (NM_007169) Human Untagged Clone

CAT#: SC115659

PEMT (untagged)-Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEMT
• Synonyms: PEAMT; PEMPT; PEMT2; PLMT; PNMT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115659 sequence for NM_007169 edited (data generated by NextGen Sequencing)
  ATGACCCGGCTGCTGGGCTACGTGGACCCCCTGGATCCCAGCTTTGTGGCTGCCGTCATC
  ACCATCACCTTCAATCCGCTCTACTGGAATGTGGTTGCACGATGGGAACACAAGACCCGC
  AAGCTGAGCAGGGCCTTCGGATCCCCCTACCTGGCCTGCTACTCTCTAAGCGTCACCATC
  CTGCTCCTGAACTTCCTGCGCTCGCACTGCTTCACGCAGGCCATGCTGAGCCAGCCCAGG
  ATGGAGAGCCTGGACACCCCCGCGGCCTACAGCCTGGGCCTCGCGCTCCTGGGACTGGGC
  GTCGTGCTCGTGCTCTCCAGCTTCTTTGCACTGGGGTTCGCTGGAACTTTCCTAGGTGAT
  TACTTCGGGATCCTCAAGGAGGCGAGAGTGACCGTGTTCCCCTTCAACATCCTGGACAAC
  CCCATGTACTGGGGAAGCACAGCCAACTACCTGGGCTGGGCCATCATGCACGCCAGCCCC
  ACGGGCCTGCTCCTGACGGTGCTGGTGGCCCTCACCTACATAATGGCTCTCCTATACGAA
  GAGCCCTTCACCGCTGAGATCTACCGGCAGAAAGCCTCCGGGTCCCACAAGAGGAGCTGA
  
  Clone variation with respect to NM_007169.2
  523 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007169 unedited
  GAGGTGCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGGCACGAGGTGCAGC
  GGGGCCCGCGTGGTGCCTCNCTGAGCGGCCCCCGGATGAAGAGATCTGGGAACCCGGGAG
  CCGAGGTAACGAACAGCTCGGTGGCAGGGCCTGACTGCTGCGGAGGCCTCGGCAATATTG
  ATTTTAGACAGGCAGACTTCTGCGTTATGACCCGGCTGCTGGGCTACGTGGACCCCCTGG
  ATCCCAGCTTTGTGGCTGCCGTCATCACCATCACCTTCAATCCGCTCTACTGGAATGTGG
  TTGCACGATGGGAACACAAGACCCGCAAGCTGAGCAGGGCCTTCGGATCCCCCTACCTGG
  CCTGCTACTCTCTAAGCGTCACCATCCTGCTCCTGAACTTCCTGCGCTCGCACTGCTTCA
  CGCAGGCCATGCTGAGCCAGCCCAGGATGGAGAGCCTGGACACCCCCGCGGCCTACAGCC
  TGGGCCTCGCGCTCCTGGGACTGGGCGTCGTGCTCGTGCTCTCCAGCTTCTTTGCACTGG
  GGTTCGCTGGAACTTTCCTAGGTGATTACTTCGGGATCCTCAAGGAGGCGAGAGTGACCG
  TGTTCCCCTTCAACATCCTGGACAACCCCATGTACTGGGGAAGCACAGCCAACTACCTGG
  GCTGGGCCATCATGCACGCCAGCCCCACGGGCCTGCTCCTGACGGTGCTGGTGGCCCTCA
  CCTACATAATGGCTCTCCTATACGAAGAGCCCTTCACCGCTGAGATCTACCGGCAGAAAG
  CCTCCGGGTCCCACAAGAGGAGCTGATTGAGCTGCCACAGCTTTGCCTGAAGGCCTGGCC
  AGCCTCCTGGCCTGCCCCAGTGGCAAGCCCTGCGCAGGACGAGAAATGTTGCCTGCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007169 unedited
  AAAACTTTGGGTGGCCTTTTATGGTGACTGGGAATGTGTGGGTTGGAGCTCAAGGCCTAT
  GTCGGCACGTCCAGGTCCCCAAGCAGCAGGTTCCAAGGCACTGGGGCAGCCCACGCCGGG
  GGCGAGCCCTGAGCAGCAGGCACCATTCTCGCCCTGCGCAGGGCCTGCCACTTGGGGCAG
  GCCAGGAGGCTGGCCAGGCCTTCAGCAAAGCTGTTGCAGCTCAATCAGCTCCTCTTGTGG
  GACCCGGAGGCTTTCTGCCGGTAGATCTCAGCGGTGAAGGGCTCTTCGTATAGGAGAGCC
  ATTATGTAGGTGAGGGCCACCAGCACCGTCAGGAGCAGGCCCGTGGGGCTGGCGTGCATG
  ATGGCCCAGCCCAGGTAGTTGGCTGTGCTTCCCCAGTACATGGGGTTGTCCAGGATGTTG
  AAGGGGAACACGGTCACTCTCGCCTCCTTGAGGATCCCGAAGTAATCACCTAGGAAAGTT
  CCAGCGAACCCCAGTGCAAAGAAGCTGGAGAGCACGAGCACGACGCCCAGTCCCAGGAGC
  GCGAGGCCCAGGCTGTAGGCCGCGGGGGTGTCCAGGCTCTCCATCCTGNGCTGGCTCAGC
  ATGGCCTGCGTGAAGCAGTGCGAGCGCAGGAAGTTCAGGAGCAGGATGGTGACGCTTAGA
  GAGTAGCAGGCCAGGTAGGGGGATCCNGAAGCCCTGCTCAGCTTGCGGGTCTTGTNGTCC
  CATCGTGCAACCACATTCCAGTAAAGCGGATTGAAGGTGATGGTGATGACGGCAGCCACA
  AAGCTGGGATCCAGGGGGTCCACGTAGCCCAGCAGCCGGGTCATAACGCAGAAGTCTGCC
  TGTCTAAAATCAATATTGCCGAGGCCTNCGCAGCAGTCAGGCCCCTGCACCGAGCTGTNC
  GNTACCTCGGCTCCCAGGGTCCCAGATCTCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007169
• ORF Size: 600 bp
• Insert Size: 1000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_007169.2, NP_009100.2
• RefSeq Size: 1008
• RefSeq ORF: 600
• Locus ID: 10400
• Domains: PEMT
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes.