Clathrin light chain (CLTA) (NM_007096) Human Untagged Clone

CAT#: SC115717

CLTA (untagged)-Human clathrin, light chain A (CLTA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLTA
• Synonyms: LCA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115717 sequence for NM_007096 edited (data generated by NextGen Sequencing)
  ATGGCTGAGCTGGATCCGTTCGGCGCCCCTGCCGGCGCCCCTGGCGGTCCCGCGCTGGGG
  AACGGAGTGGCCGGCGCCGGCGAAGAAGACCCGGCTGCGGCCTTCTTGGCGCAGCAAGAG
  AGCGAGATTGCGGGCATCGAGAACGACGAGGCCTTCGCCATCCTGGACGGCGGCGCCCCC
  GGGCCCCAGCCGCACGGCGAGCCGCCGGGGGGTCCGGATGCTGTTGATGGAGTAATGAAT
  GGTGAATACTACCAGGAAAGTAATGGTCCAACAGACAGTTATGCAGCTATTTCACAAGTG
  GATCGATTGCAGTCAGAGCCTGAAAGTATCCGTAAATGGAGAGAAGAACAAATGGAACGC
  TTGGAAGCCCTTGATGCCAATTCTCGGAAGCAAGAAGCAGAGTGGAAAGAAAAGGCAATA
  AAGGAGCTAGAAGAATGGTATGCAAGACAGGACGAGCAGCTACAGAAAACAAAAGCAAAC
  AACAGGGTGGCAGATGAAGCTTTCTACAAACAACCCTTCGCTGACGTGATTGGTTATGTC
  ACAAACATAAACCATCCTTGCTACAGCCTAGAACAGGCAGCAGAAGAAGCCTTTGTAAAT
  GACATTGACGAGTCGTCCCCAGGCACTGAGTGGGAACGGGTGGCCCGGCTGTGTGACTTT
  AACCCCAAGTCTAGCAAGCAGGCCAAAGATGTCTCCCGCATGCGCTCAGTCCTCATCTCC
  CTCAAGCAGGCCCCGCTGGTGCACTGA
  
  Clone variation with respect to NM_007096.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007096 unedited
  GGTGTTCGAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCCACAGC
  GGTGGCTGCCGGGCGTGGTGTCGGTGGGTCGGTTGGTTTTTGTCTCACCGTTGGTGTCCG
  TGCCGTTCAGTTGCCCGCCATGGCTGAGCTGGATCCGTTCGGCGCCCCTGCCGGCGCCCC
  TGGCGGTCCCGCGCTGGGGAACGGAGTGGCCGGCGCCGGCGAAGAAGACCCGGCTGCGGC
  CTTCTTGGCGCAGCAAGAGAGCGAGATTGCGGGCATCGAGAACGACGAGGCCTTCGCCAT
  CCTGGACGGCGGCGCCCCCGGGCCCCAGCCGCACGGCGAGCCGCCGGGGGGTCCGGATGC
  TGTTGATGGAGTAATGAATGGTGAATACTACCAGGAAAGTAATGGTCCAACAGACAGTTA
  TGCAGCTATTTCACAAGTGGATCGATTGCAGTCAGAGCCTGAAAGTATCCGTAAATGGAG
  AGAAGAACAAATGGAACGCTTGGAAGCCCTTGATGCCAATTCTCGGAAGCAAGAAGCAGA
  GTGGAAAGAAAAGGCAATAAAGGAGCTAGAAGAATGGTATGCAAGACAGGACGAGCAGCT
  ACAGAAAACAAAAGCAAACAACAGGGTGGCAGATGAAGCTTTCTACAAACAACCCTTCGC
  TGACGTGATTGGTTATGTCACAAACATAAACCATCCTTGCTACAGCCTAGAACAGGCAGC
  AGAAGAAGCCTTTGTAAATGACATTGACGAGTCGTCCCCAGGCACTGAGTGGGAACCGGG
  TGGCCCCGGCTGTGTGACTTTAACCCCAGTCTAGCCAGCAGGCCAAAGAAGTCTCCCGCA
  TGCGCTCAGTCCTCATCTCCTTCAGCAGCCCCCGCTGGTGCCCTGAAGACCACCCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007096 unedited
  TGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTACAGTTTGAAACAGAAATTTATTCTCAAAATAATGCACAAAAGCACAGGTTGA
  GGCTACTCTTGGGAAGCTTCCCTCCCCTTCCTTTTCCTCCTCTCCCTCCTTTTTGAATGC
  CAGGGAAAACACAGTTGAAGGAAGGAAACATGCAATCACAAACAATGAACAACTCTAAAG
  ACAAAAAGGTTTGGTCCAAAAGAACTCAACATAATTAATCCAATGACTGGGAAAAGCTTC
  ACTGAGTAGGATTAAAATATTGCAGATGTAGTGTTTCCACAGGGGGGCTCTTCAGTGCAC
  CAACGGGGCCTGCTTGAGGGAAATGAGGACTGAGCGCATGCGGGAAACATCTTTGGCCTG
  CTTGCTAAACTTGGGGTTAAAGTCACACAGCCGGGCCACCCGTTCCCACTCAGTGCCTGG
  GGACGACTCGTCAATGTCATTTACAAAGGCTTCTTCTGCTGCCTGTTCTAGGCTGTAGCA
  AGGATGGTTTATGTTTGTGACATAACCAATCACGTCAGCGAAGGGTTGTTTGTAAAAAGC
  TTCATCTGCCACCCTGTTGTTTGCTTTTGTTTTCTGTAGCTGCTCGTCCTGTCTTGCATA
  CCATTCTTCTAGCTCCTTTATTGCCTTTTCTTTCCACTCTGCTTCTTGCTTCCGAGAATT
  GGCATCAAGGGCTTCCAAGCGTTCCATTTGNTCTTCTCTCCATTTACGGATACTTTCAGG
  CTCTGACTGCAATCGATCCACTTGTGAAATAGCTGCATAACTGCCTGTTGGGACATTACT
  TTNCTGGNAGTATTCACCATTATTACTNCATNAAAGCATTCGGACCCCCCGNNNGNTCCC
  GTGCCGGTGGGGCCCGGGGCCGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007096
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007096.1, NP_009027.1
• RefSeq Size: 1105 bp
• RefSeq ORF: 747 bp
• Locus ID: 1211
• Cytogenetics: 9p13.3
• Domains: Clathrin_lg_ch
• Protein Pathways: Endocytosis, Huntington's disease, Lysosome
• Gene Summary: 'Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) represents the longest transcript variant and encodes the longest isoform (b).