CCT3 (NM_005998) Human Untagged Clone

CAT#: SC116415

CCT3 (untagged)-Human chaperonin containing TCP1, subunit 3 (gamma) (CCT3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCT3
• Synonyms: CCT-gamma; CCTG; PIG48; TCP-1-gamma; TRIC5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116415 sequence for NM_005998 edited (data generated by NextGen Sequencing)
  ATGATGGGCCATCGTCCAGTGCTCGTGCTCAGCCAGAACACAAAGCGTGAATCCGGAAGA
  AAAGTTCAATCTGGAAACATCAATGCTGCCAAGACTATTGCAGATATCATCCGAACATGT
  TTGGGACCCAAGTCCATGATGAAGATGCTTTTGGACCCAATGGGAGGCATTGTGATGACC
  AATGATGGCAATGCCATTCTTCGAGAGATTCAAGTCCAGCATCCAGCGGCCAAGTCCATG
  ATCGAAATTAGCCGGACCCAGGATGAAGAGGTTGGAGATGGGACCACATCAGTAATTATT
  CTTGCAGGGGAAATGCTGTCTGTAGCTGAGCACTTCCTGGAGCAGCAGATGCACCCAACA
  GTGGTGATCAGTGCTTACCGCAAGGCATTGGATGATATGATCAGCACCCTAAAGAAAATA
  AGTATCCCAGTCGACATCAGTGACAGTGATATGATGCTGAACATCATCAACAGCTCTATT
  ACTACCAAAGCCATCAGTCGGTGGTCATCTTTGGCTTGCAACATTGCCCTGGATGCTGTC
  AAGATGGTACAGTTTGAGGAGAATGGTCGGAAAGAGATTGACATAAAAAAATATGCAAGA
  GTGGAAAAGATACCTGGAGGCATCATTGAAGACTCCTGTGTCTTGCGTGGAGTCATGATT
  AACAAGGATGTGACCCATCCACGTATGCGGCGCTATATCAAGAACCCTCGCATTGTGCTG
  CTGGATTCTTCTCTGGAATACAAGAAAGGAGAAAGCCAGACTGACATTGAGATTACACGA
  GAGGAGGACTTCACCCGAATTCTCCAGATGGAGGAAGAGTACATCCAGCAGCTCTGTGAG
  GACATTATCCAACTGAAGCCCGATGTGGTCATCACTGAAAAGGGCATCTCAGATTTAGCT
  CAGCACTACCTTATGCGGGCCAATATCACAGCCATCCGCAGAGTCCGGAAGACAGACAAT
  AATCGCATTGCTAGAGCCTGTGGGGCCCGGATAGTCAGCCGACCAGAGGAACTGAGAGAA
  GATGATGTTGGAACAGGAGCAGGCCTGTTGGAAATCAAGAAAATTGGAGATGAATACTTT
  ACTTTCATCACTGACTGCAAAGACCCCAAGGCCTGCACCATTCTCCTCCGGGGGGCTAGC
  AAAGAGATTCTCTCGGAAGTAGAACGCAACCTCCAGGATGCCATGCAAGTGTGTCGCAAT
  GTTCTCCTGGACCCTCAGCTGGTGCCAGGGGGTGGGGCCTCCGAGATGGCTGTGGCCCAT
  GCCTTGACAGAAAAATCCAAGGCCATGACTGGTGTGGAACAATGGCCATACAGGGCTGTT
  GCCCAGGCCCTAGAGGTCATTCCTCGTACCCTGATCCAGAACTGTGGGGCCAGCACCATC
  CGTCTACTTACCTCCCTTCGGGCCAAGCACACCCAGGAGAACTGTGAGACCTGGGGTGTA
  AATGGTGAGACGGGTACTTTGGTGGACATGAAGGAACTGGGCATATGGGAGCCATTGGCT
  GTGAAGCTGCAGACTTATAAGACAGCAGTGGAGACGGCAGTTCTGCTACTGCGAATTGAT
  GACATCGTTTCAGGCCACAAAAAGAAAGGCGATGACCAGAGCCGGCAAGGCGGGGCTCCT
  GATGCTGGCCAGGAGTGA
  
  Clone variation with respect to NM_005998.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005998 unedited
  CCCATTCAGATGTGTACACGTTCATATAGGCGGCCGCGCAATTCGCACGAGGGCTTCTGT
  CTGGTTCTCTCTCTCCAGAGGTTCTGCCGGTTCCCCCAGCTCTGGGTACCCGGCTCTGCA
  TCGCGTCGCCATGATGGGCCATCGTCCAGTGCTCGTGCTCAGCCAGAACACAAAGCGTGA
  ATCCGGTAAGAAAAGTTCAATCTGGTTAACTATCAATGCTGCCAAGACTATTGCAGATAT
  CATCCGAACATGTTTGGGACCCAAGTCCATGATGAAGATGCTTTTGGACCCAATGGGAGG
  CATTGTGATGACCAATGATGGCAATGCCATTCTTCGAGAGATTCAAGTCCAGCATCCAGC
  GGCCAAGTCCATGATCGAAATTAGCCGGACCCAGGATGAAGAGGTTGGAGATGGGACCAC
  ATCAGTAATTATTCTTGCAGGGGAAATGCTGTCTGTAGCTGAGCACTTCCTGGAGCAGCA
  GATGCACCCAACAGTGGTGATCAGTGCTTACCGCAAGGCATTGGATGATATGATCAGCAC
  CCTAAAGAAAATAAGTATCCCAGTCGACATCAGTGACAGTGATATGATGCTGAACATCAT
  CAACAGCTCTATTACTACCAAAGCCATCAGTCGGTGGTCATCTTTGGCTTGCAACATTGC
  CCTGGATGCTGTCAAGATGGTACAGTTTGAGGAGAATGGTCTGAAAGAGATTGACATAAA
  AAAATATGCTAGAGTGGAAAAGATACCTGGAGGCATCATTGAAGACTCCTGTGTCTTGCG
  TGGAGTCATGATTAACCAGGATGTGACCCATTCACGTATGCGGCGCTATATCACAGACCC
  TCGCATTGTGCTGCTGGAATCTTTTCTGGATACCAGACAGGAGCAGCCCCTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005998 unedited
  AACTTCCAGGGCCGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTT
  CAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCA
  AATGACTTAAATTTAATCATCGGAAGCAAACTAAATGGAAACCTTACAATAGAGAAGAAT
  TACATGTCAGTGTCTTTTGGAAAACTGAGCTGGGACAGAAAGGGACTGGGGGCTGCCCCC
  CAACCTGATCCCTTCTGAACAAAGACGTCCACAGTGTTCCTGGCACTCTGGCTCAGGAAA
  AGGGGAGACTCTGCTGGTTCTGTGCATTGAAGTAGCCTTGCCTAGCACTCACTCCTGGCC
  AGCATCAGGAGCCCCGCCTTGCCGGCTCTGGTCATCGCCTTTCTTTTTGTGGCCTGAAAC
  GATGTCATCAATTCGCAGTAGCAGAACTGCCGTCTCCACTGCTGTCTTATAAGTCTGCAG
  CTTCACAGCCAATGGCTCCCATATGCCCAGTTCCTTCATGTCCACCAAAGTACCCGTCTC
  ACCATTTACACCCCAGGTCTCACAGTTCTCCTGGGTGTGCTTGGCCCGAAGGGAGGTAAG
  TAAACGGATGGTGCTGGCCCCACAGTTCTGGATCAGGGTACGAGGAATGACCTCTAGGGC
  CTGGGCAACAGCCCTGTATGGCCATTGTTCCACACCAGTCATGGCCTTTGGATTTTTCTG
  GTCAGGCATGGGCCACAGCCATCTCGGGAGCCCCACCCCCTGGCACAGCTTGAGGGTCAG
  NAGACATTGCGACACCTTGCATGGCATCTGGAGGTTGCGTTCTATTCCGAGAGATCTCTT
  GCTACCCCCCAGAGAAATGGGGCAGCCCTTGGGTCTTGCATCAGGGAGAAAGAAAGTATC
  ANTCCAATTCTTGATTTCACAGCCTGCTCTGTTCAAAATTTTTTCTCAGTCCTTGGCGGT
  GGATATCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005998
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005998.3, NP_005989.3
• RefSeq Size: 2096 bp
• RefSeq ORF: 1638 bp
• Locus ID: 7203
• Cytogenetics: 1q22
• Domains: cpn60_TCP1
• Gene Summary: 'The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (a).