MMP11 (NM_005940) Human Untagged Clone

CAT#: SC116425

MMP11 (untagged)-Human matrix metallopeptidase 11 (stromelysin 3) (MMP11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP11
• Synonyms: SL-3; ST3; STMY3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116425 sequence for NM_005940 edited (data generated by NextGen Sequencing)
  ATGGCTCCGGCCGCCTGGCTCCGCAGCGCGGCCGCGCGCGCCCTCCTGCCCCCGATGCTG
  CTGCTGCTGCTCCAGCCGCCGCCGCTGCTGGCCCGGGCTCTGCCGCCGGACGTCCACCAC
  CTCCATGCCGAGAGGAGGGGGCCACAGCCCTGGCATGCAGCCCTGCCCAGTAGCCCGGCA
  CCTGCCCCTGCCACGCAGGAAGCCCCCCGGCCTGCCAGCAGCCTCAGGCCTCCCCGCTGT
  GGCGTGCCCGACCCATCTGATGGGCTGAGTGCCCGCAACCGACAGAAGAGGTTCGTGCTT
  TCTGGCGGGCGCTGGGAGAAGACGGACCTCACCTACAGGATCCTTCGGTTCCCATGGCAG
  TTGGTGCAGGAGCAGGTGCGGCAGACGATGGCAGAGGCCCTAAAGGTATGGAGCGATGTG
  ACGCCACTCACCTTTACTGAGGTGCACGAGGGCCGTGCTGACATCATGATCGACTTCGCC
  AGGTACTGGCATGGGGACGACCTGCCGTTTGATGGGCCTGGGGGCATCCTGGCCCATGCC
  TTCTTCCCCAAGACTCACCGAGAAGGGGATGTCCACTTCGACTATGATGAGACCTGGACT
  ATCGGGGATGACCAGGGCACAGACCTGCTGCAGGTGGCAGCCCATGAATTTGGCCACGTG
  CTGGGGCTGCAGCACACAACAGCAGCCAAGGCCCTGATGTCCGCCTTCTACACCTTTCGC
  TACCCACTGAGTCTCAGCCCAGATGACTGCAGGGGCGTTCAACACCTATATGGCCAGCCC
  TGGCCCACTGTCACCTCCAGGACCCCAGCCCTGGGCCCCCAGGCTGGGATAGACACCAAT
  GAGATTGCACCGCTGGAGCCAGACGCCCCGCCAGATGCCTGTGAGGCCTCCTTTGACGCG
  GTCTCCACCATCCGAGGCGAGCTCTTTTTCTTCAAAGCGGGCTTTGTGTGGCGCCTCCGT
  GGGGGCCAGCTGCAGCCCGGCTACCCAGCATTGGCCTCTCGCCACTGGCAGGGACTGCCC
  AGCCCTGTGGACGCTGCCTTCGAGGATGCCCAGGGCCACATTTGGTTCTTCCAAGGTGCT
  CAGTACTGGGTGTACGACGGTGAAAAGCCAGTCCTGGGCCCCGCACCCCTCACCGAGCTG
  GGCCTGGTGAGGTTCCCGGTCCATGCTGCCTTGGTCTGGGGTCCCGAGAAGAACAAGATC
  TACTTCTTCCGAGGCAGGGACTACTGGCGTTTCCACCCCAGCACCCGGCGTGTAGACAGT
  CCCGTGCCCCGCAGGGCCACTGACTGGAGAGGGGTGCCCTCTGAGATCGACGCTGCCTTC
  CAGGATGCTGATGGCTATGCCTACTTCCTGCGCGGCCGCCTCTACTGGAAGTTTGACCCT
  GTGAAGGTGAAGGCTCTGGAAGGCTTCCCCCGTCTCGTGGGTCCTGACTTCTTTGGCTGT
  GCCGAGCCTGCCAACACTTTCCTCTGA
  
  Clone variation with respect to NM_005940.3
  113 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005940 unedited
  AAACTGGGATTTGTAATACGACTTTATATAGGCGGCCGACGAATTCGCACGAGGCCGGGG
  CGGATGGCTCCGGCCGCCTGGCTCCGCAGCGCGGCCGCGCGCGCCCTCCTGCCCCCGATG
  CTGCTGCTGCTGCTCCAGCCGCCGCCGCTGCTGGCCCGGGCTCTGCCGCCGGACGTCCAC
  CACCTCCATGCCGAGAGGAGGGGGCCACAGCCCTGGCATGCAGCCCTGCCCAGTAGCCCG
  GCACCTGCCCCTGCCACGCAGGAAGCCCCCCGGCCTGCCAGCAGCCTCAGGCCTCCCCGC
  TGTGGCGTGCCCGACCCATCTGATGGGCTGAGTGCCCGCAACCGACAGAAGAGGTTCGTG
  CTTTCTGGCGGGCGCTGGGAGAAGACGGACCTCACCTACAGGATCCTTCGGTTCCCATGG
  CAGTTGGTGCAGGAGCAGGTGCGGCAGACGATGGCAGAGGCCCTAAAGGTATGGAGCGAT
  GTGACGCCACTCACCTTTACTGAGGTGCACGAGGGCCGTGCTGACATCATGATCGACTTC
  GCCAGGTACTGGCATGGGGACGACCTGCCGTTTGATGGGCCTGGGGGCATCCTGGCCCAT
  GCCTTCTTCCCCAAGACTCACCGAGAAGGGGATGTCCACTTCGACTATGATGAGACCTGG
  ACTATCGGGGATGACCAGGGCACAGACCTGCTGCAGGTGGCAGCCCATGAATTTGGCCAC
  GTGCTGGGGCTGCAGCACACAACAGCAGCCCAAGCCCTGATGTCCGCCTTCTACACCTTT
  CGCTACCCACTGAGTCTCAGCCCAGATGACTGCAGGGGCGTTCAACACCTATATGGCCAG
  CCCTGCCCACTGTCACCTCCAGACCCCAACCTTGGCCCCCAGCTGGGATAGACACCATGA
  GATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005940 unedited
  ACCGGGCCGCAATCTAGTATCGAGCNTTTTCTTTTTTTTTTTTTTTTTTTTAAAAAACAA
  CTGTGTTTAATGACAATCCTCAGTTTAAAAAAAAAAAAGAAGAAGAAGGTTTATACACAC
  TGTACACACATTTACAATGGCTTTGGAGGATAGCAGTGCTGCGAAAAGGGCTTCAGGAGG
  ATCCGGCCTGGGACAGGATTGAGGTATGTTGCAGCCTCCAGGGCCTGGGGTCTCCTGCAT
  GAAAAATACCCCTCCCCATTTGACTGTGAACTTTTTGGCCTGGATTCTGGAGAACAGATT
  TCCAGGATTGTCAGCCAGAAGGCAGACAGATGCAGGCACCTACCAAGACCTGACCTCAGG
  AAGTGGCCCTGCCCTACAGCCCAGTTGCTCATCCAGGGCTGAAGGCCATGGGGCCCCAGC
  ACCCTTGCTTCAGTGCCAGCCCCTGGAAGGAACCTCACAACAGGGATACAGCAAGGACAC
  TCCAGTTCCCCCAGTCCTGCCATGGTGCTACCCTGAGGGACAGGGATGGAGACAGGGCAG
  CCAGGTTTGCCAGGACCTGCATAGCGGGCCCAAGACTGCCCTTCCTCTTAAGTCATGCCA
  AAGCCTCCCTGCCCAGTCTGAGACAGTCGCTGGCAGGTGACCACGACCTGCGTGGCCCTT
  CCCGCAGTTGTCATGGTGTTTGTACCCCACCCCATCTCCCTGAGGAGACATGGGCTAAGT
  CCCATGCCTGGTGCCCACAGCCACCAAGATGGCCATGGGTCTTTACCCTGATATTCGAGG
  CCTGCAAGGGTCATCACCCCTGAGGGCTTCCAGCCCATGTCATAGAAAGTGTTGGCAGCT
  CCGCCATCCAATATTCAGGACCACGAAACGGGAAGCCTTCAAGCCTTCCTTCAAGGTCAA
  CTCTCATAAGCGGCCGCCAGGATTAGCTAACATCACTTCTGAAGGAGGGGATTCCAAGGA
  CCCTTTCATAATGCCTTGGGGCAGTATGCTACACCGGGCTGGTGGATCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005940
• Insert Size: 2160 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005940.3, NP_005931.2
• RefSeq Size: 2276 bp
• RefSeq ORF: 1467 bp
• Locus ID: 4320
• Cytogenetics: 22q11.23
• Domains: hemopexin, Peptidase_M10, ZnMc
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the protein.