TAF11 (NM_005643) Human Untagged Clone

CAT#: SC116607

TAF11 (untagged)-Human TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa (TAF11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF11
• Synonyms: MGC:15243; PRO2134; TAF2I; TAFII28
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005643, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGATGCCCACGAGTCGCCCTCCGACAAAGGTGGAGAGACAGGGGAGTCGGATGAGACGGCCGCTG
  TGCCCGGGGACCCGGGGGCTACCGACACCGATGGAATCCCAGAGGAAACTGACGGAGACGCAGATGTGGA
  CTTGAAAGAAGCTGCAGCGGAGGAAGGCGAGCTCGAGAGTCAGGATGTCTCAGATTTAACAACAGTTGAA
  AGGGAAGACTCATCATTACTTAATCCTGCAGCCAAAAAACTGAAAATAGATACCAAAGAAAAGAAAGAGA
  AAAAGCAGAAAGTAGATGAAGATGAGATTCAGAAGATGCAAATCCTGGTTTCTTCTTTTTCTGAGGAGCA
  GCTGAACCGTTATGAAATGTATCGCCGCTCAGCTTTCCCTAAGGCAGCCATCAAAAGGCTGATCCAGTCC
  ATCACTGGCACCTCTGTGTCTCAGAATGTTGTTATTGCTATGTCTGGTATTTCCAAGGTTTTCGTCGGGG
  AGGTGGTAGAAGAAGCACTGGATGTGTGTGAGAAGTGGGGAGAAATGCCACCACTACAACCCAAACATAT
  GAGGGAAGCCGTTAGAAGGTTAAAGTCAAAAGGACAGATCCCTAACTCGAAGCACAAAAAAATCATCTTC
  TTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005643 unedited
  TCAGCATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCCTCA
  GGCATCTCCGCGATCTCCTCTCCCCTCCAATCCTATCCGTGATGGACGATGCCCACGAGT
  CGCCCTCCGACAAAGGTGGAGAGACAGGGGAGTCGGATGAGACGGCCGCTGTGCCCGGGG
  ACCCGGGGGCTACCGACACCGATGGAATCCCAGAGGAAACTGACGGAGACGCAGATGTGG
  ACTTGAAAGAAGCTGCAGCGGAGGAAGGCGAGCTCGAGAGTCAGGATGTCTCAGATTTAA
  CAACAGTTGAAAGGGAAGACTCATCATTACTTAATCCTGCAGCCAAAAAACTGAAAATAG
  ATACCAAAGAAAAGAAAGAGAAAAAGCAGAAAGTAGATGAAGATGAGATTCAGAAGATGC
  AAATCCTGGTTTCTTCTTTTTCTGAGGAGCAGCTGAACCGTTATGAAATGTATCGCCGCT
  CAGCTTTCCCTAAGGCAGCCATCAAAAGGCTGATCCAGTCCATCACTGGCACCTCTGTGT
  CTCAGAATGTTGTTATTGCTATGTCTGGTATTTCCAAGGTTTTCGTCGGNGAGGTGGTAG
  AAGAAGCACTGGATGTGTGTGAGAAGTGGGGAGAAATGCCACCACTACAACCCCAAACAT
  ATGAGGGAAGCCGTTAGAAGGTTAAAGTCAAAAGGACAGATCCCTAACTCGAAGCACAAA
  AAAATCATCTTTCTTCTAGACCAAAGTCTAGAAAGGCCCTATGTACTGACGGAAGAAGTA
  TTGGTTCCAGACTTCCTATTAGACTGGCTGCATTGGTGCTTTAATATCTCCAGCCTCCCA
  GGATTCCATGATGATTNTAANGGCTTTCTCAAAACTCTGAATTTGTCCCACCTAAAAGGA
  TGGACCCTGATGAAACTGGCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005643 unedited
  GCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGTTGGAAAACAGTTTA
  ATGATCACTCACCAAAATCCACAGGAAGAATCTTAAATGTTTACAAGCACCAATTATTCT
  GCTATTCCTGCCATCACCGCATCCTTCATGGTAGAGTATCACAAGTAAAAGTTTCTGGTT
  GTTTCATCTACTTAAAACCAGATATAAGAAACAACCTAAGTCTTAGCAACTTCAGGCTTC
  AATGTGAAACCATTAAAGCCCTCAGCACTTTAGGAGGCTGAGGCAGGAGGACTGCTTGAA
  GCCAGGAGTTCACGACCAGCCTGGGCAACAAAGCAAGACCCCATCTCCATAAAAAATAAA
  AATAAGTTAGCTGGGCACAGTAGTGTGTGCCTGTAGTCCTAGCTACTCAGGAGACTGAGG
  TGGGAGGGTCACTTAAGCCCAGGAGTTCAAGGCTGCAGTCATGCCGCTGCACTCCAGCCT
  AGGTGATAGAGCAAGACCCTATCTCANACAAAACGAAACCAAAACAAAACAAAACTCTCT
  TGGAAAGGTTTTCTCTCTGAAAGCAGTTCCCCTGTCCAGAAGATGCTTATGGCCCACGTA
  TCTTCTTTCAACTGGTCAAGACAGTTAAATACTTCAAAATGCCCCCAAGAGTCCCAAAAT
  TTAGTCAAGGCAAGTATCAATCAGGCTACATACTTTCTAGGTGTGACAAATATCAGAGTT
  TTGAGAAAGACTTTAAAATCATCCTGGAATTCTTGGNAGCCTGAGATACTTAAGCCACCA
  TGCAGACAGTTTTATAAGAAGTTCTGAACCAATACTCTTCCGTCAGTAACATAGGCCTTC
  TAGACCTTGGTCTAAAGAAGAGATTTTTTTGGCCTCAGTTAGGGAACTGCCCTTTGACTT
  TAACCTCTAAACGGTCCTTAATAGTTGGGTTGGAAGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005643
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005643.2, NP_005634.1
• RefSeq Size: 1599 bp
• RefSeq ORF: 636 bp
• Locus ID: 6882
• Cytogenetics: 6p21.31
• Domains: TAFII28
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).