BCAT1 (NM_005504) Human Untagged Clone
CAT#: SC116691
BCAT1 (untagged)-Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1
"NM_005504" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | BCAT1 |
Synonyms | BCATC; BCT1; ECA39; MECA39; PNAS121; PP18 |
Vector | pCMV6-XL6 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116691 sequence for NM_005504 edited (data generated by NextGen Sequencing)
ATGAAGGATTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAG GTGGTGGGGACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAA AAACCAGACCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTG GAGTGGTCCTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCA TTGCACCCTGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCA TTTCGAGGAGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATG TATCGCTCTGCTGTGAGGGCAACTCTGCCGGTATTTGACAAAGAAGAGCTCTTAGAGTGT ATTCAACAGCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTG TATATTCGTCCTACATTCATTGGAACTGAGCCTTCTCTTGGAGTCAAGAAGCCTACCAAA GCCCTGCTCTTTGTACTCTTGAGCCCAGTGGGACCTTATTTTTCAAGTGGAACCTTTAAT CCAGTGTCCCTGTGGGCCAATCCCAAGTATGTAAGAGCCTGGAAAGGTGGAACTGGGGAC TGCAAGATGGGAGGGAATTACGGCTCATCTCTTTTTGCCCAATGTGAAGCAGTAGATAAT GGGTGTCAGCAGGTCCTGTGGCTCTATGGAGAGGACCATCAGATCACTGAAGTGGGAACT ATGAATCTTTTTCTTTACTGGATAAATGAAGATGGAGAAGAAGAACTGGCAACTCCTCCA CTAGATGGCATCATTCTTCCAGGAGTGACAAGGCGGTGCATTCTGGACCTGGCACATCAG TGGGGTGAATTTAAGGTGTCAGAGAGATACCTCACCATGGATGACTTGACAACAGCCCTG GAGGGGAACAGAGTGAGAGAGATGTTTGGCTCTGGTACAGCCTGTGTTGTTTGCCCAGTT TCTGATATACTGTACAAAGGCGAGACAATACACATTCCAACTATGGAGAATGGTCCTAAG CTGGCAAGCCGCATCTTGAGCAAATTAACTGATATCCAGTATGGAAGAGAAGAGAGCGAC TGGACAATTGTGCTATCCTGA Clone variation with respect to NM_005504.6 >OriGene 5' read for NM_005504 unedited
CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT TATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCG GCACGAGGGGACCGCGCTCTGCAGCCGCAGACCCGGTCCACACGGCCAGGGGCTACGACC CTTGGGATCTGCCCTCCGCTCAGCTCGAGCTTCCCTCGTGGCCGACGGAACAATGAAGGA TTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAGGTGGTGGG GACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAAAAACCAGA CCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTGGAGTGGTC CTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCATTGCACCC TGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCATTTCGAGG AGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATGTATCGCTC TGCTGTGAGGGCAACTCTGCCGGTATTTGACANAGAAGAGCTCTTAGAGTGTATTCAACA GCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTGTATATTCG TCCTACATTCATTGGAACTGAGCCTTCTCTTGGNAGTCAGAGCCTACCCAGCCCTGCTCT TTGTACTCTTGAGCCCAGTGGACCTTATTTTTNCAGTGGACCTTNATNCAGTGTCCCTGT GGCCATCCNAGTATGTAGAGCCTGGAAAGGTGNACTGNNGACTGCAGATGGGNAGGNAAT ACCGCTCATCTCTTTTGCCCATGTGAGCAGTAATNAATGGTGGTCACCAGGTCCTGTGGC TTTATGGAGAGGACATCAGATCACTGAGTGGAACTA >OriGene 3' read for NM_005504 unedited
GTCTACTATGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTCTTAC ATTTTTTCTTACAACAATCTTTTGAGAAAAGAAAGAGAATGTTTACCAGGTAGAAAAAAC CTGATTCCTTAACAAGACTTCTCAGTCACTTGTTTCTCTTTCCCTTTCACTTTTCCTTTG TGCTTTTGAAAACAAAGGCTAAAGCAGGTGTTATAAATTTATTCATTCCTGTGCTAGAGA GCATGGAACACATCTCTGAAGTCAACTCTTCTTTTGGTGTATCACGAGTTGAATCCTTAA TAAATGACTGCCACAAAGAAAATTTCTAACAGCAAATCTTTTTAGGAACTGCTCAGGTTT TGCCTTCGTCTTGACACTTCATTTTAAAATCTTGGTTTCTACTGATGTGTTTTCAAAAAA GCTGACAAACTCGGTCTGAAATGTAGACACTGAAAAAAGTGAAAGGAAGAGAACATGCAA TATAAGTTAATCATTATCATTTTAAGTGCCACCAGTTCTGTTTATATTAATGCACTTGTG AATTGCTGAATCTCATTTGATCAACCCCATTAAACATTAAATAATTGCAGCCAATTATGC CAATATCAGCCACAATATTAATTTGTTGATCTTTTTGAGCAGTTAGCTTCACATGTTCCT AAAGCTAGACCATCTTATGCCTTTAAACGATCAATTAGTGTGACAATAACCTAATCCTTG AAGAATGGATAATTGCCTAGTTATAATGTTGGCACTCCTGCTGTATTAAACCACTGGCAT TAAACCAATAGTGCACACAGAAAAAAAAAGGGCCAAAGTTCCCTGTTAAATCTTTTTAAC AAGAAAACGTCCAGGGCAATTTCCGGGGCTATTACCTAAAGGACAAGGTGGGATTGGGGT CTCCTTTTAAGGAGAACCTCCCGTTTTAAAACACTCTTC |
Restriction Sites | NotI-NotI |
ACCN | NM_005504 |
Insert Size | 4700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005504.4, NP_005495.2 |
RefSeq Size | 8191 bp |
RefSeq ORF | 1161 bp |
Locus ID | 586 |
Cytogenetics | 12p12.1 |
Domains | aminotran_4 |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation |
Gene Summary | 'This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]' Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219229 | BCAT1 (Myc-DDK-tagged)-Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1 |
USD 420.00 |
|
RG219229 | BCAT1 (GFP-tagged) - Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1 |
USD 460.00 |
|
RC219229L1 | Lenti ORF clone of Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC219229L2 | Lenti ORF clone of Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC219229L3 | Lenti ORF clone of Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC219229L4 | Lenti ORF clone of Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1, mGFP tagged |
USD 620.00 |
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