PI 3 Kinase p85 beta (PIK3R2) (NM_005027) Human Untagged Clone

CAT#: SC116980

PIK3R2 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 2 (beta) (PIK3R2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIK3R2
• Synonyms: MPPH; MPPH1; p85; p85-BETA; P85B
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005027 edited
  ATGGCGGGCCCTGAGGGCTTCCAGTACCGCGCTCTGTACCCGTTCCGCCGGGAGCGGCCG
  GAGGACCTGGAGCTGCTGCCCGGCGACGTGCTGGTAGTGAGCCGGGCGGCCTTGCAGGCG
  CTGGGCGTGGCCGAGGGTGGCGAGCGCTGCCCACAGAGCGTGGGCTGGATGCCCGGCCTC
  AACGAGCGCACACGGCAGCGAGGTGACTTCCCTGGCACCTATGTGGAGTTCCTGGGGCCC
  GTGGCCCTGGCCCGGCCCGGCCCTCGCCCACGGGGCCCCCGCCCACTGCCCGCCAGGCCC
  CGTGATGGGGCCCCTGAGCCAGGCCTCACACTCCCCGACTTGCCCGAGCAGTTCTCCCCA
  CCTGATGTGGCTCCCCCTCTTCTGGTGAAGCTTGTGGAGGCCATTGAAAGGACAGGGCTG
  GACAGCGAATCTCACTACCGCCCGGAGCTGCCCGCACCGCGTACAGACTGGTCCCTGAGC
  GACGTGGATCAGTGGGACACGGCAGCCCTGGCTGACGGCATTAAGAGCTTCCTGCTGGCA
  CTGCCCGCGCCGCTCGTGACCCCCGAGGCCTCGGCCGAGGCGCGCCGGGCCCTGCGGGAG
  GCCGCGGGGCCCGTGGGGCCGGCGCTGGAGCCACCGACGCTGCCGCTGCACCGCGCGCTC
  ACGCTGCGCTTCCTGCTCCAGCACCTGGGCCGCGTGGCCCGCCGCGCCCCGGCCCTGGGT
  CCCGCGGTCCGGGCCCTGGGCGCCACCTTTGGGCCGCTGCTGCTGCGCGCGCCGCCGCCG
  CCGTCCTCGCCGCCGCCAGGGGGCGCTCCCGACGGGAGTGAGCCCAGCCCTGACTTCCCG
  GCGCTGCTGGTGGAGAAGCTGCTTCAGGAACACTTGGAAGAGCAGGAGGTTGCGCCCCCA
  GCGCTGCCGCCTAAACCCCCCAAGGCAAAGCCGGCCCCCACAGTCCTGGCCAATGGAGGG
  AGCCCACCCTCCCTGCAGGATGCTGAGTGGTACTGGGGGGACATTTCAAGGGAGGAGGTG
  AACGAGAAACTCCGGGACACTCCCGATGGCACCTTCCTAGTCCGAGATGCTTCTAGCAAG
  ATCCAGGGCGAGTACACGCTGACCCTCAGGAAAGGCGGGAACAATAAGCTGATCAAGGTC
  TTCCACCGAGATGGGCACTATGGCTTCTCAGAGCCACTCACCTTCTGCTCCGTTGTGGAC
  CTCATCAATCACTACCGCCACGAGTCTCTGGCCCAGTACAATGCCAAGCTGGACACACGG
  CTCCTCTACCCTGTGTCCAAATACCAGCAGGACCAGATTGTCAAGGAGGACAGCGTGGAG
  GCAGTGGGCGCCCAGCTTAAGGTCTATCACCAGCAGTACCAGGACAAGAGCCGCGAGTAT
  GACCAGCTTTATGAAGAGTACACACGGACCTCCCAGGAGCTGCAGATGAAGCGTACTGCA
  ATTGAGGCCTTCAATGAGACTATCAAGATCTTTGAAGAGCAGGGCCAGACTCAAGAGAAA
  TGCAGCAAGGAATACCTGGAGCGCTTCCGGCGTGAGGGCAACGAGAAAGAGATGCAAAGG
  ATCCTGCTGAACTCCGAGCGGCTCAAGTCCCGCATTGCCGAGATCCATGAGAGCCGCACG
  AAGCTGGAGCAGCAGCTGCGGGCCCAGGCCTCGGACAACAGAGAGATCGACAAGCGCATG
  AACAGCCTCAAGCCGGACCTCATGCAGCTGCGCAAGATCCGAGACCAGTACCTCGTGTGG
  CTCACCCAGAAAGGCGCCCGGCAGAAGAAAATCAACGAGTGGCTGGGGATTAAAAATGAG
  ACTGAGGACCAGTACGCACTCATGGAGGACGAGGACGATCTCCCGCACCACGAGGAACGC
  ACTTGGTACGTGGGCAAGATCAACCGCACGCAGGCAGAGGAGATGCTGAGTGGCAAGCGG
  GATGGCACCTTCCTCATCCGCGAGAGCAGCCAGCGGGGCTGCTACGCCTGCTCCGTGGTA
  GTGGACGGCGACACCAAGCACTGCGTCATCTACCGCACGGCCACCGGCTTCGGCTTCGCG
  GAGCCCTACAACCTGTACGGGTCGCTGAAGGAGCTGGTGCTGCACTACCAGCACGCCTCG
  CTGGTGCAGCACAACGACGCGCTCACCGTCACCCTGGCGCACCCAGTGCGCGCCCCGGGC
  CCCGGCCCGCCGCCTGCCGCCCGCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005027 unedited
  TAACCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGACGAGGCAATGGCCCAGTGACCTGACACCACACCACCAACTCCCTCCCACCA
  GCTGACGAATGGTGGACCCAGTGACGAGTGGCCCTTGTAAGGGTCATGGAATAATTTGAA
  GCGAGGCATGAGCGGCCCCTGTGGTCGCCTGTGACTGCTGGAGATAGAGGTCCCAGCACC
  CCAAGCCAACCCAGCGGACCCTCCCAGCCCTGCTTCAACCAATGGGGCCAGTGGGGCTCC
  AAGCAGCCACCTAACCATCCAGACCCCACCCCACTCACGCGGCCATGGCGGGCCCTGAGG
  GCTTCCAGTACCGCGCTCTGTACCCGTTCCGCCGGGAGCGGCCGGAGGACCTGGAGCTGC
  TGCCCGGCGACGTGCTGGTAGTGAGCCGGGCGGCCTTGCAGGCGCTGGGCGTGGCCGAGG
  GTGGCGAGCGCTGCCCACAGAGCGTGGGCTGGATGCCCGGCCTCAACGAGCGCACACGGC
  AGCGAGGTGACTTNCCTGGCACCTATGTGGAGTTNCTGGGGCCCGTGGCCCTGGCCCGGC
  CCGCCCTCGCCCACGGNNGCCCCGCCACTGCCCGCCAGGCCCCGTGATGGGGCCCCTGAG
  CCAGGCCTACACTTCCCGACTTGCCCGACAGTTCTCCCCACTGATGTGGCTCCCCTCTTC
  TGTGAAGCTGTTGAGGCCCATGAAGGACAGGGCTGGACAGCGAATCTCACTACGCCCGGA
  CTGCCGCACGCGTACGACTGGTCCTGACGAGTGGATCGTGGGACACGCAGCCTGCCTGAC
  GCATTAGAGCTTCTGCTGCACTGCCGCGCCGTCGGACCCCGAGCTCGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005027 unedited
  GAACTACTATGTACGTGGCCGCGTTCTATGATCGGTTTTTTTTTTTTTTTTTTGACGTTT
  GATGTTTTATATCATATCAATAACAACGTACCGTACAAAACCTGCCTCCCAGCTCGTGGG
  GCAGGCAGTGTGGTGGCCAAGGGCAGCGTATAAAACCAGGGCTCTTGTCCCAAAAGAGGA
  GAGGAAAGAGGGTTTTGCAGTCAAGGAAAAACAGAATCAAAACCCAACATGATCGAAGAG
  AAGCCCTGAATCTGCATCAGAGGGAGGGGCCGTGGGCAGTGTCGCAACTTGGCCACTGCG
  GCGGCACCAGTACGGGGAGCTGTGATTCCTAAGAGGGGTTGGGGCCAGCTCACAAGTTTA
  TTTATTTATTTTTTTGAGACGGAGGAGTCTCCCTCTGTTACCCAAGCTGGGAGTGCANTG
  GCACGATCTCTGCTCACTGCAACCTCCATCTCCTGGGTTCAAGAGATTCTCACGCCTCAG
  CCTCCCAAGTAGCTGGGATTACAGGCGGCCGCCACCACGCCCGGCTAATTTTTGTATTTT
  AGTAGATGCGGGGTTTTGCCATTTTGGCCAAATTGGCCTTCAACTCCCGACCTTCAAAGA
  TCCGCCCGTCTTGGCCTCCCCAAGTGCTGGGATTACAGGCATGAACCACCGTGCCTGGCC
  TGCGAGTTTAATTTTTTTTTCGGGGTGGGGTGAACTTTTCTGCACCGGCCCCTTTGGGGG
  GTAACCCGGTGGCGGGTTTTACCTTGGGGTTTGGAGGGGAATTTGGGTTCTGTGGGGCGG
  GTCCCCCCCGGCCCCCATGGTACCGGCCCGGGTTTTTTGGAACCACAAAATTGAATTTCC
  TTTCTGGCCCTGCCCCAGGTCTATGGGCTTAAAAATAAGGGGCCCCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005027
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005027.2, NP_005018.1
• RefSeq Size: 3953 bp
• RefSeq ORF: 2187 bp
• Locus ID: 5296
• Cytogenetics: 19p13.11
• Domains: RhoGAP, SH2, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: 'Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. [provided by RefSeq, Apr 2019]'
  Transcript Variant: This variant (1) represents the protein-coding variant.