PIGH (NM_004569) Human Untagged Clone

CAT#: SC117284

PIGH (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGH
• Synonyms: GPI-H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117284 sequence for NM_004569 edited (data generated by NextGen Sequencing)
  ATGGAGGATGAGCGGAGCTTTTCGGATATCTGCGGCGGCCGCCTGGCGCTGCAGCGCCGC
  TACTACTCCCCGTCCTGCCGGGAATTCTGCCTCAGCTGCCCTCGGCTCTCGCTGCGTTCG
  CTCACCGCTGTCACCTGCACGGTGTGGCTGGCGGCCTACGGACTCTTCACCCTCTGCGAG
  AACAGCATGATCCTCTCTGCTGCCATCTTCATCACCCTCTTAGGTCTGCTTGGTTATCTC
  CATTTTGTGAAGATTGATCAGGAGACTCTGTTAATCATTGATTCCCTTGGCATTCAGATG
  ACTTCATCTTATGCTTCAGGCAAAGAAAGCACTACCTTCATAGAAATGGGCAAGGTCAAG
  GATATTGTCATCAATGAGGCCATTTACATGCAGAAGGTGATTTACTACCTCTGCATCTTA
  TTGAAAGATCCAGTGGAACCACATGGGATATCCCAAGTAGTACCCGTCTTCCAGAGTGCC
  AAGCCCCGGCTGGACTGCTTGATTGAAGTATACAGGAGCTGCCAGGAGATCCTGGCACAC
  CAGAAAGCCACATCAACAAGCCCATGA
  
  Clone variation with respect to NM_004569.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004569 unedited
  TTTTGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGGCGAGCGCA
  GTGCAGCGCCGCGCGGTGCGGGCGGCCGAGTGGGGGCGTCATGGAGGATGAGCGGAGCTT
  TTCGGATATCTGCGGCGGCCGCCTGGCGCTGCAGCGCCGCTACTACTCCCCGTCCTGCCG
  GGAATTCTGCCTCAGCTGCCCTCGGCTCTCGCTGCGTTCGCTCACCGCTGTCACCTGCAC
  GGTGTGGCTGGCGGCCTACGGACTCTTCACCCTCTGCGAGAACAGCATGATCCTCTCTGC
  TGCCATCTTCATCACCCTCTTAGGTCTGCTTGGTTATCTCCATTTTGTGAAGATTGATCA
  GGAGACTCTGTTAATCATTGATTCCCTTGGCATTCAGATGACTTCATCTTATGCTTCAGG
  CAAAGAAAGCACTACCTTCATAGAAATGGGCAAGGTCAAGGATATTGTCATCAATGAGGC
  CATTTACATGCAGAAGGTGATTTACTACCTCTGCATCTTATTGAAAGATCCAGTGGAACC
  ACATGGGATATCCCAAGTAGTACCCGTCTTCCAGAGTGCCAAGCCCCGGCTGGACTGCTT
  GATTGAAGTATACAGGAGCTGCCAGGAGATCCTGGCACACCAGAAAGCCACATCAACAAG
  CCCATGAGCCCCAGCGTTCAGAAGGCCAGCATTGTCTTCCATGGGAGATGACTCTTAAGC
  CATAGGGGCTGGTTTTCCGTACTCCCAACATCANGTGGACACAGTCCTAGGAACCATATG
  GATGTAGTGCATCTTTAGACCATAGAGCANGTGACTGGNAATCTAACTCAGTATATTTTC
  GTGTATTATAGTGGTTTCCTTGGAGGNNTTTGCCTACTTTACCCAAGGNAGGGGAGACCT
  NNAGAATTTNGACACAGTATGTCAAAAGTAATGTCAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004569 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATATTTGCTTATTTATTCTT
  TGTTAACATGAAAGTCCCATGTCTGAAAACCAAAGTCCAATTTCTGTCTGGCCTTTTGTC
  TCATCCTTCTTGGCAAAAGTAGCTTTTGAACTGATATAAAAAAAAATGCTGAGTAACAGA
  AAAGTATTAATGTGCTTGACACCATGACTGAAATACTATGATCTTGTTTGTCAATAAAAA
  GCAGCTATCTGTGAACCAGGTAACTGTGTGTTTTGGAAGATCTGTTTATTAACAGTAAAT
  AAATAAGCCCTGTACAGAACACAGGCACTAGGTTGACAGACTCGTCTTTTGTAGGACATT
  TCTTCCTGCTAACCAGTACTGAAGGGCTTGTTTTTTTCGTAACTTTACACAAGGGGTACT
  ATTGGAAAATATAGCTTTCTCAAGACATCTGATGTCAGTTTCCCATTGTTTTGCTTCACA
  AACATCAACAAAGTAAACCTGAACATGCTTAGCAATTTCCGAAAGTGTTCTTTGCTGACA
  TTACTTTTGACATACTGTGTCAAAATTCTTAAGGTCTCCCCTCCTTTGGTAAAGTAGGCA
  AAACCTTACAAGGAAAACACTATAATACACGGAAATATACTGAGTTAGATTTCCAGTCAC
  CTGCTCTATGGCTCTAAGATGCACTACATCCATAATGGTTCCTAGGACTGTGTCCACCTG
  ATGGTTTGGAGTCGGGAAAACCAGCCCCTATGGCTTAAGATCATCTCCCATGGAAGACAT
  GCTGGCCTTCTGACGCTGGGGCTCATGGCTTGTTGATGTGCCTTCTGGGTGCCAGGATCT
  CCTGACACTCCTGTTACCTCATCAACCATCACCCGGGGCTTGCCCTTTGGAAAGGGTCTA
  CCTGGATACCCTGGGGTCCCTGATCTTCATAAAGCAAGGGGAAACCCTTTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004569
• Insert Size: 1310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004569.2, NP_004560.1
• RefSeq Size: 1393 bp
• RefSeq ORF: 567 bp
• Locus ID: 5283
• Cytogenetics: 14q24.1
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008]'